锤头状核酶

抗HIF-1αmRNA锤头状核酶设计及其基因克隆

Design of a hammerhead ribozyme targeting HIF-1 α mRNA and cloning of its gene

锤头状核酶降低小鼠胰岛细胞经CD95途径的凋亡

Hammerhead ribozyme reducing the apoptosis of mouse islet cells via CD95 pathway

锤头状核酶对短片段雄激素受体mRNA的体外切割作用

Cleavage of short fragment of androgen receptor mRNA by hammerhead ribozyme in vitro

锤头状核酶RCP对HBV的P基因体外转录物的作用

Effect of hammerhead ribozyme ( RCP ) on HBV P gene in vitro

丁型肝炎病毒基因组RNA包埋锤头状核酶的活性研究

Investigation on activities of hammerhead ribozyme embedded in genomic RNA of hepatitis delta virus

作用于HBV（adr亚型）RNA的tRNA-包埋锤头状核酶的研究

TRNA embedded Hammerhead Ribozymes Mediate Destruction of HBV ( Subtype adr ) in vitro

特异性切割c-myc基因mRNA锤头状核酶抑制血管平滑肌细胞增殖的研究

Inhibition of Vascular Smooth Muscle Cell Proliferation by Hammerhead Ribozyme that Specifically Cleaves C-myc mRNA

锤头状核酶对不同长度MDRlmRNA的体外切割

Hammerhead ribozyme-mediated cleavage of mdrl mRNA with different length

结论特异性切割AR的锤头状核酶在体外对底物RNA有良好的切割活性，为应用核酶阻断雄激素受体基因表达奠定了基础。

Conclusion The specific anti-AR hammerhead ribozyme could cleave the substrate efficiently in vitro , and its activity against AR would be studied further in cell culture .

肝内胆管癌hTR反义RNA及锤头状核酶基因真核表达载体的构建

Building antisense RNA gene and hammerhead ribozyme gene sequence in the liver inside bile duct cancer cell into the real expression vector

锤头状核酶调控CD95介导小鼠皮肤成纤维细胞凋亡

Modulation of CD95-mediated apoptosis of mouse skin fibroblast cells by hammerhead ribozyme

目的设计一个抗缺氧诱导因子1α（HIF-1α）mRNA的锤头状核酶，并构建它的基因表达载体，以便用于肿瘤的基因治疗。

Objective To design a hammerhead ribozyme targeting HIF-1 α mRNA and clone its gene into an expression vector for future use in the study of tumor gene therapy .

锤头状核酶抑制Raji细胞表面主要组织相容性复合物Ⅱ类抗原的表达

Inhibitive effect of hammerhead ribozyme against C ⅱ TA on the expression of MHC ⅱ on Raji cells

针对HBV基因组设计了三种锤头状核酶&RS3、RC2和RC1。

Three hammerhead ribozymes ( RS3 , RC2 and RC1 ) targeting to the HBV genome have been designed .

抗Caspase-7锤头状核酶在原代肝细胞内的切割活性研究

Research of cleavage activity of hammerhead ribozyme targeting caspase-7 in primary cultured liver cells

目的设计并合成抗小鼠半胱氨酸蛋白酶-7（caspase-7）的锤头状核酶，并对它们的体外表达与体外切割活性进行研究。

Objective To design hammerhead ribozymes against mouse caspase-7 and to study their expression and cleavage activity in vitro .

目的：研究用丁型肝炎病毒（HDV）作为载体携带乙型肝炎病毒（HBV）特异性的锤头状核酶所构建的重组体，在细胞体系及转染动物模型中对HBV基因表达和复制的影响。

Objective : To study the effects of hammerhead ribozymes delivered by hepatitis delta virus ( HDV ) targeted on hepatitis B virus ( HBV ), in cell culture and an animal model , on the HBV gene expression and replication .

在已发现的核酶中，锤头状核酶最据代表性，因为它结构简单、分子小、易于设计、合成及转移，并且对底物RNA序列的要求不像发卡型核酶那样严格。

In all discovered ribozymes , the hammerhead ribozyme is the best representative , because it has many excellences including simple structure , small molecule , being apt to design , synthesize and transfer , further more it doesn ` t like hairpin ribozyme that has specifical to its substrate .

根据锤头状核酶的作用模式，设计、合成并克隆了特异性切割马铃薯卷叶病毒（PotatoLeafrollVirus，PLRV）中国分离株（PLRV-Ch）复制酶基因负链RNA的二价核酶序列。

Based on the functional model of the hammerhead RNAs , a divalent ribozyme was designed for cleavage of Potato leafroll virus ( PLRV ) RNA , targeting at two sites on the negative strand of PLRV replicase gene .

目的检测犬贾第虫病毒介导的锤头状核酶对犬贾第虫滋养体核仁功能性蛋白KRR1基因体外转录体切割效率。

Objective To detect the cleavage activity of Giardia canis virus ( GCV ) transfer vector-mediated hammerhead ribozyme for KRR1 in vitro transcript .

锤头状核酶阻断雄激素受体基因表达的研究

The hammerhead ribozyme-induced blocking effects on gene expression of androgen receptor

针对bcr/abl的锤头状核酶的骨髓净化作用

The Effect of Anti-bcr-abl Hammerhead Ribozyme on Bone Marrow Purging

自我剪切锤头状核酶体外切割HPV11/E2的研究

Studies on Cleavage of HPV11 / E2 Gene by Self - cleaved Hammerhead Ribozyme

人端粒酶催化亚单位锤头状核酶诱导肝癌细胞凋亡的作用

Hammerhead ribozyme against human telomerase catalytic subunit ( hTERT ) induced apoptosis of liver cancer cells

结论所设计的锤头状核酶可对NHE1mRNA进行特异性切割，减少其表达，使其活性下降，从而诱导细胞酸化。

Conclusion Hammerhead ribozyme can cleave the target NHE 1 mRNA specifically , reduce the expression of NHE 1 mRNA and induce intracellular acidosis .

目的设计针对小鼠内质网应激凋亡途径中Caspase-12mRNA的锤头状核酶（Rz138、Rz218），通过靶基因及核酶的体外转录、切割，进行核酶活性鉴定，评估其应用前景。

Objective To design and synthesize ribozymes targeting 138 and 218 sites of the mRNA nucleotide of mouse caspase-12 , a key intermedium of ER stress mediated apoptosis , and to identify their activities through in vitro transcription and cleavage .