非同位素标记

非同位素标记脆弱类杆菌DNA探针的研究

A study of non radioactive DNA probe for detection of Bacteroides fragilis

非同位素标记DNA探针初步研究

The application of nonisotopic DNA probe in forensic science

目的建立用非同位素标记探针杂交测定端粒DNA长度的方法，并探讨其法医学的应用价值。

Objective To measure telomere length by hybridization with non - radio - isotopic - labeled probes and study its application in forensic medicine .

非同位素标记探针杂交分析白细胞端粒DNA长度

Telomere length measurement of human leukocyte in peripheral blood by non-radio - isotopic hybridization ; Study on the Aggregation of Lable-free Nucleic Acid Probe Induced by DNA

在基因文库中任意选择53个克隆，利用专一的PCR进行DNA扩增以及dig非同位素标记，用于和草菇基因组DNA杂交。

Fifty three random clones from the genomic library were non - radioactivity digoxigenin - labeled by specific PCR and characterized by Southern hybridization .

应用聚合酶链式反应体外DNA放大和同位素或非同位素标记的探针在我国南方进行了β地中海贫血的产前诊断。

A prenatal diagnosis of β thalassemia was carried out in South China using a in vitro DNA amplification by a polymerase chain reaction and isotope-labeled or nonradioactive probes .

对采用外源基因原核显微注射法生产的基因转移山羊，用一种新的DNA非同位素标记法即地高辛配基标记DNA探针作外源基因整合检测。

Gene transfer goats produced by the method of microinjection foreign gene into pronuclear were made integrated detection with a novel nonradioactive DNA labeling method , namely DNA probe labeling with Digoxigenin .

采用非同位素标记原位杂交的方法，经图文分析系统的扫描结果显示：初生时，卵巢内ESRmRNA几乎不表达，15、30、45日龄阳性率上升，60日龄稍有下降；

Results of image manipulation analysis system indicated that ESR mRNA almost are not detected at birth in the ovary ; ESR mRNA expression increased from PNDs 15 to 45 , and declined slightly on PNDs 60 ;

现在，很多研究工作集中于非同位素标记研究。

Recently , nonisotopic detection systems have been the center of the study work .

用凝集素和非同位素标记底物测定核心α－1，6－岩藻糖转移酶及其应用

Determination of Core α - 1,6-Fucosyltransferase Using Lectin and Non-isotope Substrate and Its Application

采用非同位素地高辛标记cRNA探针对人睾丸、附睾和输精管组织冰冻切片进行原位杂交。

Methods : In situ hybridization of human testis , epididymis and vas deferens tissues was performed with digoxin labeled 5 α reductase type 2 cRNA probe .

2针对放射性同位素检测法的缺点，文章提出并比较了几种常用的非放射性同位素标记方法。

Second , aim at flaws of method radioactive marker , same methods of non - radioactive marker were raised and compared .