非同位素标记
- non-isotope labeling
![非同位素标记](../cache/1188213.jpg)
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非同位素标记脆弱类杆菌DNA探针的研究
A study of non radioactive DNA probe for detection of Bacteroides fragilis
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非同位素标记DNA探针初步研究
The application of nonisotopic DNA probe in forensic science
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目的建立用非同位素标记探针杂交测定端粒DNA长度的方法,并探讨其法医学的应用价值。
Objective To measure telomere length by hybridization with non - radio - isotopic - labeled probes and study its application in forensic medicine .
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非同位素标记探针杂交分析白细胞端粒DNA长度
Telomere length measurement of human leukocyte in peripheral blood by non-radio - isotopic hybridization ; Study on the Aggregation of Lable-free Nucleic Acid Probe Induced by DNA
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在基因文库中任意选择53个克隆,利用专一的PCR进行DNA扩增以及dig非同位素标记,用于和草菇基因组DNA杂交。
Fifty three random clones from the genomic library were non - radioactivity digoxigenin - labeled by specific PCR and characterized by Southern hybridization .
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应用聚合酶链式反应体外DNA放大和同位素或非同位素标记的探针在我国南方进行了β地中海贫血的产前诊断。
A prenatal diagnosis of β thalassemia was carried out in South China using a in vitro DNA amplification by a polymerase chain reaction and isotope-labeled or nonradioactive probes .
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对采用外源基因原核显微注射法生产的基因转移山羊,用一种新的DNA非同位素标记法即地高辛配基标记DNA探针作外源基因整合检测。
Gene transfer goats produced by the method of microinjection foreign gene into pronuclear were made integrated detection with a novel nonradioactive DNA labeling method , namely DNA probe labeling with Digoxigenin .
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采用非同位素标记原位杂交的方法,经图文分析系统的扫描结果显示:初生时,卵巢内ESRmRNA几乎不表达,15、30、45日龄阳性率上升,60日龄稍有下降;
Results of image manipulation analysis system indicated that ESR mRNA almost are not detected at birth in the ovary ; ESR mRNA expression increased from PNDs 15 to 45 , and declined slightly on PNDs 60 ;
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现在,很多研究工作集中于非同位素标记研究。
Recently , nonisotopic detection systems have been the center of the study work .
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用凝集素和非同位素标记底物测定核心α-1,6-岩藻糖转移酶及其应用
Determination of Core α - 1,6-Fucosyltransferase Using Lectin and Non-isotope Substrate and Its Application
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采用非同位素地高辛标记cRNA探针对人睾丸、附睾和输精管组织冰冻切片进行原位杂交。
Methods : In situ hybridization of human testis , epididymis and vas deferens tissues was performed with digoxin labeled 5 α reductase type 2 cRNA probe .
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2针对放射性同位素检测法的缺点,文章提出并比较了几种常用的非放射性同位素标记方法。
Second , aim at flaws of method radioactive marker , same methods of non - radioactive marker were raised and compared .