咽拭子标本

对28份咽拭子标本直接进行DNA提取和PCR扩增，有17份标本PCR扩增L3部分基因呈阳性，经序列测定和分析，也为腺病毒3型。

DNA extraction and PCR were carried out directly from 28 throat swab specimens , and 17 positives were also identified as adenovirus type 3 by sequencing analysis .

用Hep-2细胞从患儿20份咽拭子标本中分离到12株病毒，经PCR扩增腺病毒L3部分基因及其PCR产物的序列测定和分析，证实这12株病毒为腺病毒3型；

12 viral isolates from 20 throat swab specimens using Hep-2 cells were identified as adenovirus type 3 by L3 gene PCR and sequencing analysis .

从10份咽拭子标本中分离出5株麻疹病毒，经核酸序列分析鉴定，均为H1基因型。

Wild-type measles viruses were isolated from 10 throat swabs , the sequence analysis indicated that all of the 5 isolates belong to H1 genotype .

结果35份患者的咽拭子标本中用PCR扩增同时检测到了12份腺病毒（阳性率为34.3%）和15份B型流感病毒（阳性率为42.9%），呼吸道合胞病毒核酸检测阴性。

Results Adenovirus nucleic acid in 12 swabs ( 34.3 % ) and influenza B virus nucleic acid in 15 swabs ( 42.9 % ) were detected from total 35 patient nasopharyngeal swab samples whereas no RSV nucleic acid was found .

麻疹病人咽拭子标本的麻疹病毒分离结果

Results of measles virus isolation from throat swab samples from patients with measles

方法采用苛养菌及链球菌鉴定的常规方法对呼吸道感染患儿的咽拭子标本进行分离、培养与鉴定。

Methods routine methods were used to isolate and identify fastidous bacteria and Streptococcus from samples of children 's throat swabs .

35份咽拭子标本分离出15株病毒，分离阳性率42.9%。分离病毒经鉴定为腺病毒3型。

15 strains of human adenovirus type 3 were isolated from 35 throat swab samples and the isolation rate of the virus was 42.9 % ( 15 / 35 ) .

采用流感病毒、麻疹病毒、腮腺炎病毒和风疹病毒作为报告病毒，并对80例上呼吸道感染患者的咽拭子标本进行验证测试。

Using influenza virus , mumps virus , measles virus and rubella virus to test the respiratory virus detection DNA microarray , and 80 throat swab specimens as a testing samples were also detected .

方法采用常规方法进行302份咽拭子标本培养和菌种鉴定，选择15种抗菌药物，用纸片扩散法对所分离的菌种进行药敏试验。

METHODS Totally 302 pharyngeal samples were cultured and isolated , and the tests of antibiotic susceptibility were ( performed ) for isolated strains to 15 kinds of antibiotics by using of the paper diffusion method .

结果克雷伯菌属的检出以咽拭子和痰标本为主；

RESULTS Klebsiella were isolated from pharynx and sputum .

咽拭子及痰液标本肺炎支原体培养对支原体肺炎诊断的影响

Comparison of mycoplasma culture for sputum and throat swab for diagnosis of mycoplasmal pneumonia in children