斑点杂交

通过反向Northern斑点杂交法进一步筛选消减文库，对阳性克隆进行序列测定和同源性分析；

Reverse Northern dot blot was carried out to further screen the subtracted cDNA library .

PCR结合反向斑点杂交法检测石蜡包埋组织中的曲霉感染

Detection and identification of Aspergillus species from formalin-fixed , paraffin-embedded human tissues by PCR and dot blot hybridization

应用斑点杂交法测定血清中乙型肝炎病毒的DNA

Detection of HBV DNA in Serum with Spot Hybridization

不同浓度消毒剂与病毒液作用不同时间后提取DNA作病毒核酸斑点杂交检测。

DNA was extracted after different time of treatment by disinfectants of different concentration .

斑点杂交法和Southern杂交法检测敏感性相同，为100fg；

Dot hybridization has the same sensitivity as Southern hybridization .

重组DNA技术斑点杂交法检测生物检材的性别

The biological material sexing by dot hybridization

应用PCR和斑点杂交产前诊断α&地中海贫血

Prenatal Diagnosis of α - Thalassemia by PCR and Southern Blot

斑点杂交试验直接检测血清HBVdna的临床意义

Clinical significance of direct spot hybridization in serum HBV DNA

检测烟草中烟草花叶病毒的RNA斑点杂交法

RNA dot hybridization for detecting tobacco mosaic virus in tobacco leave

RNA斑点杂交检测核酶基因在卵巢癌细胞中的表达；

Ribozyme expression was confirmed by RNA dot blot ;

采用RNA斑点杂交和RT-PCR技术检测OPNmRNA的表达。

OPN mRNA expression was detected with RNA dot blot and RT-PCR .

所用的验证方法是杂交，包括斑点杂交和NorthernBlot。

The method for experimental validation is hybridization , which comprises dot blot and northern blot .

用DNA斑点杂交法检测对虾及其饵料和环境生物携带白斑综合症病毒状况的调查

Investigation and study on carrying status of White Spot Syndrome Virus ( WSSV ) in shrimps and other aquatic animals by dot-blot hybridization

分别用病毒双链探针或单链负义探针，与吸附在纤维膜上的组织DNA进行斑点杂交。

TTV was detected by PCR , and dot hybridization was done with virus double DNA strand probe or single antisense strand probe .

利用斑点杂交法和RT-PCR技术检测甘蔗花叶病毒

Detection of - Sugarcane mosaic virus - by dot hybridization and RT PCR technique

与定性PCR及分子斑点杂交检测比较，定量PCR的灵敏度更高。

Its sensitivity of this method was higher than that of the qualitative PCR and dot - hybridization analysis .

斑点杂交生物素法检测流行性出血热病毒RNA

Detection of Epidemic Hemorrhagic Fever Virus ( Hantanvirus ) RNA Genome with Biotin-Labeled Probe by Dot Hybridization

应用RT-PCR、斑点杂交法和SDS-PAGE检测水稻黑条矮缩病毒

Detection of rice black-streaked dwarf Fijivirus by RT-PCR , dot-blot hybridization and SDS-PAGE

目的建立PCR结合生物素标记的寡核苷酸探针斑点杂交技术，鉴定临床常见的真菌。

Objective To establish a method for detecting and identifying clinically important fungi by PCR combined with oligonucleotide probe hybridization .

用原位杂交和RNA斑点杂交法检测人原发性肝癌层粘蛋白mRNA

Detection of Laminin mRNA in Human Primary Liver Cancer Tissue by in situ Hybridization and RNA Dot Blot Hybridization

结果表明，长片段靶序列反向斑点杂交可用于HBV耐药基因多位点变异检测。

So long-segment DNA reverse dot hybridization could be used in detected multi-mutations in lamivudine-resistant HBV . 2 .

用长臂光敏生物素标记的cDNA探针核酸斑点杂交检测马铃薯纺锤块茎类病毒

Detection of Potato Spindle Tuber Viroid by Space-arm Photobiotin Labelled cDNA Probe and Nucleic Acid Spot Hybridization

HPV核酸探针斑点杂交技术在诊断尖锐湿疣中的应用

Dot hybridization technique in diagnosis of condyloma acuminata

总RNA斑点杂交显示转染细胞中有稳定的tk基因转录产物。

Transcription-al product of the tk gene was also detected by RNA dot blot hybridization .

夹心ELISA和斑点杂交试验检测鸭血清中鸭乙型肝炎病毒的比较

Comparison and Analysis of Detection for Duck Hepatitis B Virus in Duck Sera by Sandwich ELISA and Dot blot Hybridization

结果表明两个亚型的RT-PCR斑点杂交方法比RT-PCR灵敏度高出100到1000倍。

The results showed its sensitivity was 100 to 1000 times as much as that of RT-PCR method .

方法应用PCR方法检测145株临床分离肠球菌中esp的携带率，并用斑点杂交确证。

Methods : PCR and dot blot were used to detect the incidence of esp in 145 clinical isolates .

结果DNA斑点杂交、RNA斑点杂交、原位杂交和免疫组织化学染色均显示转染组有阳性信号；

Results the positive signals were observed in transfected keratinocytes by DNA dot blot , RNA dot blot , hybridization in situ and immunohistochemistry .

13例患者鼻咽分泌物中的RSV基因得到扩增，并经Southern印迹与斑点杂交证实。

RSV genes in nasal aspirates from 13 patients were amplified and identified by Southern Blot Hybridization .

另8例正常者作为对照组。用斑点杂交法测定风心病患者及正常左室心肌组织的Ⅰ、Ⅲ型胶原mRNA的含量。

Type ⅰ and ⅲ collagen mRNA levels in the myocardium from the patients and 8 controls were determined by dot blot hybridization .