发卡结构

对所鉴定的227种miRNA进行基因组定位，共预测到214个基因组序列，能够形成稳定的miRNA前体二级发卡结构。

The 214 genomic sequences , encompassing the 227 miRNAs identified above , were predicted to be capable of forming stable hairpin structures characteristic of miRNA precursors . 3 .

问题的关键是设计出合适的发卡结构，其遗传序列必须与其替代的DNA序列一致，才能迅速准确地结合其编码的RNA成为双链RNA分子。

It is just a question of making a hairpin with an appropriate genetic sequence - one that is the same as the missing strand of the original DNA - so that the hairpin will combine eagerly with the messenger to form a double-stranded molecule .

为了产生免标记的荧光信号，该设计将能够形成G-四分体结构的DNA序列的一部分隐藏在DNA发卡结构中。

In order to acquire label-free fluorescence signal , we hide partial sequence of the G-quadruplex into one of the hairpins .

目标DNA能够触发发卡结构级联自组装，使荧光基团和淬灭剂的距离增加，荧光淬灭效率降低，荧光增强。

The target DNA can trigger the cascaded self-assembly of hairpins , which makes the distance between the fluorophore and quencher increase . The fluorescence quenching efficiency decreases , and the fluorescence intensity enhances .

该方法首先根据目标DNA的长度和序列设计两个DNA发卡结构，并在其中一个发卡结构的茎部末端对称的位置上标记上荧光基团和淬灭剂。

First , according to the length and sequence of the target DNA , we design two DNA hairpins , and label fluorophore and quencher in the symmetrical location of the stem end of one hairpin .

当发卡结构被催化自组装后，G-四分体序列才能完全暴露，形成的G-四分体结构能结合荧光小分子，发出强烈的荧光。

When the hairpins are catalyzed to self-assembly , the whole sequence of the G-quadruplex is exposed , and the formed G-quadruplex can bind small fluorescent molecule , producing strong fluorescence .

特殊的发卡结构使分子信标具有很强的特异性识别靶标序列的能力，目前已成为分子生物学和生物技术中一种强有力的研究工具。

Specific molecular beacon hairpin structure has highly specific recognition ability to the target sequence . And it has become a powerful research tools in the filed of molecular biology and biotechnology .

发卡探针构象的变化导致发卡结构打开与引物杂交。

The conformational change in the hairpin-probe causes the opening of a hairpin structure and the hybridization to primer .