双向凝胶电泳

收集细胞蛋白并进行SDS-PAGE及双向凝胶电泳。

After lysis , with these hepatocytes protein proceed SDS-PAGE and two-dimensional gel electrophoresis .

对不同发育时期的水稻胚蛋白质，进行了单向SDS聚丙烯酰胺凝胶电泳和双向凝胶电泳分析。

Rice embryo proteins at different developmental stages were analysed by one and two-dimensional gel electrophoresis .

双向凝胶电泳分析三尖杉酯碱诱导K562细胞凋亡

Two-dimensional Polyacrylamide Gel Electrophoresis Analysis of Apoptosis in K562 Cells Induced by Harringtonine

方法采用固相pH梯度双向凝胶电泳分离UVA辐射前后人皮肤成纤维细胞的总蛋白质，凝胶经银染显色后，用ImagingMaster2D图像分析软件进行比较分析，识别差异表达的蛋白质。

After silver staining , the differential expression proteins were analyzed by using Imaging Master 2D analysis ware .

双向凝胶电泳（two-dimensionalelectrophoresis，2-DE）是基于质谱测定的蛋白质组学的标准方法。

Two dimensional gel electrophoresis ( 2-DE ) is the standard of Mass spectrometry-based proteomics .

收获肝星状细胞，提取蛋白质，用固相pH梯度双向凝胶电泳方法进行蛋白质点的分离，获得分辨率和重复性均较好的双向电泳银染图谱。

Two dimensional polyacrylamide gel electrophoresis is used to separate protein spots , and the good 2-DE pattern including high resolution and reproducibility was obtained .

用双向凝胶电泳分析低浓度烷化剂N-甲基-N′-硝基-N-亚硝胍引起的人羊膜FL细胞蛋白质的表达差异

Differential protein expression induced by low-concentration N-methyl-N ′ - nitro-N-nitrosoguanidine in FL cells using two-dimensional gel electrophoresis

方法：用双向凝胶电泳分离成年雄性ICR小鼠睾丸总蛋白；

Methods : Protein from adult ICR mouse testes was extracted by two dimensional electrophoresis .

双向凝胶电泳图谱的建立为进一步进行DRG细胞质膜蛋白质组学的研究提供了重要的基础。

The present experimental results provided important basis for further researches of cell membrane proteomics of rat 's DRG .

目的：建立血清蛋白质组双向凝胶电泳（2-DE）技术。

Objective : To establish a two-dimensional polyacrylamide gel electrophoresis ( 2-DE ) technique for serum proteomics .

【结果】1.优化获得了重复性和分辨率较好的正常健康受试者及脓毒症患者血小板蛋白质组双向凝胶电泳（2-DE）图谱。

Platelet 2-DE profiles of normal healthy volunteers and septic patients with high resolution and reproducibility were obtained . 2 .

利用固相pH梯度双向凝胶电泳（2D-PAGE）分别分离妊娠第3天（3d）、第5天（5d）、第7天（7d）小鼠子宫内膜的总蛋白质；

Total proteins of endometrium from mice pregnant for 3,5 and 7 days were analyzed by IPG 2D-PAGE ( two-dimensional gel electrophoresis ) and imaging analysis .

组织（或细胞）的蛋白质提取效率直接影响蛋白质双向凝胶电泳（2-DE）的分辨率。

Protein extraction from tissue or cells is a key step to achieve high-resolution protein separation in two dimensional electrophoresis ( 2-DE ) .

目的：（1）建立和优化玻璃体（vitreoushumor，VH）双向凝胶电泳-质谱技术。

Objective ( 1 ) To establish and optimize the two-dimensional electrophoresis / Mass Spectrometry ( 2-DE / MS ) technique for human vitreous humor ( VH ) proteomics .

方法应用MicroBio-SpinColumn过滤纯化血清蛋白，去除白蛋白和IgG，利用双向凝胶电泳（2-DE）分析评价血清蛋白过滤效果。

Methods Elimination of serum albumin and IgG was achieved by filtering with Micro Bio-Spin column and the efficiency of purification evaluated by two-dimensional polyacrylamide gel electrophoresis ( 2-DE ) .

婴幼儿二尖瓣关闭不全的成形术方法：应用双向凝胶电泳（2DE）技术对6例DA和6例NA中膜蛋白质组进行分离和比较，寻找两组标本的差异表达蛋白。

Methods : Two-dimensional electrophoresis ( 2DE ) was used to separate the proteomes of DA from six TAD patients undergoing repair surgery and six control NA from six organ donators without aortic diseases .

2DEMS途径的自动化，多维色谱整合串联质谱的使用，弥补了一些用双向凝胶电泳分离蛋白质的技术缺陷；

The automation of 2DE MS approach and the use of multidimensional liquid chromatography ( LC ) and tandem mass spectrometry ( MS / MS ) have overcome many disadvantages of two dimensional gel electrophoresis .

结果建立了稳定的血清白蛋白和IgG去除方法和双向凝胶电泳技术，且质谱证实2-DE图谱中低丰度蛋白质分辨率增加，减少了高丰度蛋白对其的干扰。

Results The method of removing serum albumin and IgG and 2-DE was established stably , increasing sharpness of separation of low-abundance proteins and decreasing the disturbing of high abundance proteins in 2-DE which were confirmed by MS.

目的：建立人口腔扁平苔藓（OLP）及正常黏膜组织的双向凝胶电泳图谱，分析其差异表达的蛋白质，筛选分子标记物。

Objective : To establish the two-dimensional Gel electrophoresis profiles with high resolution and reproducibility from human oral lichen planus and paired normal oral mucosa tissues , and identify the differential expression proteins .

结果表明，在3种常用的蛋白质提取方法中，硫脲裂解液对细胞蛋白质的溶解性最佳，相对更适合于提取MCF-7细胞的蛋白质，并与双向凝胶电泳条件更兼容。

Based on above results , we concluded that the improved lysis buffer has the best protein solubilization ability , which renders it much more suitable for cellular protein extraction from MCF-7 , and is more compatible with the conditions of 2-DE.

方法：采用双向凝胶电泳（2-DE）对敏感和耐药患者的外周血淋巴细胞进行蛋白质组差异分析。

Methods : High resolution two-dimensional polyacrylamide gel electrophoresis ( 2-DE ) was used to analyse lymphocytes in the peripheral blood of the patients with APL sensitive to ATRA and the patients with APL resistant to ATRA .

目的评价两种样品制备方法对血清蛋白质双向凝胶电泳（2-DE）分离效果的影响，建立高分辨率、高重复性的血清2-DE图谱，为鉴定疾病相关血清蛋白质奠定基础。

Objective To evaluate the effects of two sample preparation methods on two-dimensional polyacrylamide gel electrophoresis ( 2-DE ) - based serum protein separation , and produce high-resolution and reproducible 2-DE images for identifying disease-related serum protein .

方法采用双向凝胶电泳（2-DE）技术和计算机辅助的图像分析方法，对食管鳞癌转移淋巴结与正常淋巴结组织蛋白质进行分离和比较分析。

Methods Two dimensional polyacrylamide gel electrophoresis ( 2-DE ) technique and computer-assisted image analysis were used to separate the protein spots and analyze the differential expression of proteome in the metastatic lymph nodes and normal lymph nodes .

结果应用DIGE进行分离后，成功获得了蛋白质组分辨率高、重复性好的双向凝胶电泳图谱；

Results Two dimensional gel electrophoresis patterns of plasma proteins with high-resolution and reproducibility were successfully obtained using DIGE .

为探索建立适用于人乳腺癌细胞株MCF-7蛋白质提取的最佳条件，比较目前在双向凝胶电泳中常用的3种蛋白质提取方法对MCF-7细胞总蛋白的提取效率。

Three routine cellular total protein extraction methods were compared in order to determine an optimal one for human breast cancer cell line MCF-7 . The cultured MCF-7 cells were lysed by M-PER kit , standard lysis buffer or improved lysis buffer , respectively .

用淋巴细胞分离液获取两组单个核细胞标本并裂解获得其内表达的蛋白质，进行双向凝胶电泳（2-DE），保证每例样本有3次独立重复试验。

Obtain and splitting individual nucleus cells by the lymphocyte separation fluid and cell cracking liquid and acquire all of the protein in cells . Separate these proteins by the method of 2-DE. Guarantee 3 independent repeated experiments for every case . 3 .

目的：建立人鼻咽癌低分化细胞系CNE2双向凝胶电泳图谱，初步分析其蛋白质表达情况。

Objective To establish a two dimensional polyacrylamide gel electrophoresis ( 2 DE ) map of human low differentiation nasopharyngeal carcinoma ( NPC ) cell line CNE 2 proteome .

双向凝胶电泳（2-DE）具有高分辨率、高通量等特点，已被广泛地用于蛋白质组的分离.但是它在分离疏水性蛋白质和碱性蛋白质时却遇到了极大的挑战。

Two dimensional gel electrophoresis ( 2 DE ) has the property of high resolution , high throughput , so it has been used for the isolation of proteins widely , but it has continued to be a great challenge to separate hydrophobic proteins and alkaline proteins .

结论双向凝胶电泳技术和MALDI-TOF-MS是进行脂肪组织蛋白质组研究的有效手段，分析表达差异的蛋白质可以进一步明确肥胖症、2型糖尿病病因，为药物治疗提供新的靶点。

Conclusions : Two-dimensional electrophoresis and MALDI-TOF-MS are effective means in the protein profiling analysis of white adipose tissue , the differentially expressed proteins may play an important role during the development of obesity and type 2 diabetes and serve as a new target of diabetes therapy .

奶牛血浆蛋白质组学研究中双向凝胶电泳条件的优化

Optimization on Two-dimensional Electrophoresis for Plasma Proteomic Study in Dairy Cow