酶切

通过酶切、Southern杂交鉴定重组质粒。

The positive clone was identified by digestion and Southern analysis .

酶切、PCR和DNA测序鉴定。

Digestion , PCR and DNA sequencing .

结果：pcDNA3.1-IL-10酶切鉴定的电泳结果显示，pcDNA3.1-IL-10质粒有一个560bp左右的插入片断，大小和IL-10cDNA大致符合

IL-10-cDNA fragment was obtained through cleaving pUC-T-IL-10cDNA by reconstriction enzymes .

鲇鱼线粒体DNA的酶切图谱

Studies on restriction enzyme map of mitochondrial DNA from Silurus asotus

用Red介导的同源重组结合酶切改构质粒DNA

Reconstruction of plasmid DNA with Red-mediated homologous recombination combined with restriction digestion

通过质粒双酶切和DNA测序证实该重组质粒构建正确。

This was confirmed by cleavage of restriction endonuclease and DNA sequencing .

丰鲤及其双亲线粒体DNA限制性酶切图谱的研究

Study on Mitochondrial DNA of Feng Carp and Its Parents

质粒DNA提取与酶切方法的比较研究

Methods Research of Distilling and Enzyme - cut Plasmid DNA

犬Ⅰ型腺病毒DNA的酶切分析及分子克隆

Restriction Enzyme Analysis and Molecular Cloning of CAV-1 DNA

骨肉瘤基因组DNA的提取及部分酶切分析

Genomic DNA Extraction from osteosarcoma and partial digestion analysis

其中两个克隆并用限制酶切分析及DNA序列测定做进一步鉴定。

Two of them were further characterized by restriction analysis and DNA sequencing .

紫菜基因组DNA提取及酶切

Extraction and restriction digestion of genomic DNA from Porphyra

鹅肝脏线粒体DNA的限制性内切酶酶切图谱

Restriction Endonuclease Cleavage Map of Goose Liver Mitochondrial DNA

青鱼线粒体DNA限制性酶切图谱的研究

Study on Restriction Map of mtDNA from Mylopharyngodon piceus

正常和细胞质雄性不育水稻线粒体DNA限制性内切酶的酶切图谱分析

Restriction endonuclease analysis of mitochondrial DNA from normal and the cytoplasmic male-sterile rice

犬Ⅱ型腺病毒DNA的快速提取及限制性酶切分析

Rapid Preparation and Restriction Enzyme Analysis of the DNA of Canine Adenovirus Type 2

鲫鱼肌细胞线粒体DNA经上述两种限制性内切酶酶切后，皆被切成2个片段。

Auratus mtDNA was cleaved into 2 fragments .

大麦DNA单限制性酶切选择性扩增多态性技术及其优化

Establishing and Optimizing Selective Amplified DNA Fragments System Using Single Restricted Enzyme in Barley

对重组子测序结果表明，实现了DNA小片段和酶切位点的定向引入。

The results showed that the sites and low molecular weight DNA were successfully introduced .

幽门、空肠弯曲菌长期保存前后染色体DNA酶切图谱的分析

Restriction Endonuclease DNA Analysis of Campylobacter pylori and jejuni Strains before and after Long-Term Storage

PCR、双酶切鉴定以筛选PET15b-HSP65、PET15b-HSP70阳性重组表达质粒。

The recombinant plasmids are identified by PCR and double digestion .

用酶切和PCR的方法对重组质粒进行鉴定。

Used restrict enzyme and PCR to verify the reconstructed plasmid .

经菌落PCR和质粒酶切鉴定后，将目的基因进行序列测定。

Recombinants were confirmed by colony PCR and restriction enzyme digestion .

鲤鱼、鲫鱼肌细胞线粒体DNA的限制性内切酶酶切图谱比较

Restriction Endonuclease Cleavage Maps of Mitochondrial DNAs from the Muscles of Cyprinus carpio and Carassius auratus

我国4个旋毛虫分离株DNA限制性酶切片断长度差异的比较研究

Restriction Fragment Length Differences ( RFLDs ) Analysis of DNA Isolates of Trichinella spiralis from China

广西、海南和云南三地微小按蚊DNA的限制酶切片段长度多态性分析

Restriction fragment length polymorphism studies on the Anopheles minimus DNA from guangxi , hainan and Yunnan

赫坎按蚊种团内各亲缘种间基因组重复DNA的限制酶切长度差异

Restriction fragment length differences of genomic repetitive DNA from five sibling species of Anopheles HYRCANUS group

间日疟原虫DNA重组克隆的酶切鉴定和DNA转印杂交分析

Identification and analysis on Plasmodium vivax DNA fragments cloned by restriction endonuclease digestion and Southern blot hybridization

扩增产物经纯化后直接克隆到PMD18-T载体系统中，经PCR及酶切法鉴定，所得的重组质粒中含有495bp的片段。

After purification , the DNA fragment was cloned into PMD-18T vector directly .

挑取克隆，扩尹增，抽提质粒并经PCR、酶切及测序鉴定。

The clones were identified by PCR , restriction digestion and sequence analysis .