荧光标记

AFLP荧光标记和银染技术的比较分析

Comparison between fluorescence labeling and silver-staining techniques of AFLP

该方法不需要荧光标记，且金纳米粒子修饰简单，建立了一种简便快速且价格低廉的DNA突变的检测方法。

So we can detect single-base DNA mutation . This method does not need fluorescence labeling , and Au nanoparticles modification is simple . So we established one kind rapid , convenient and low-cost method for detection of DNA mutation .

而且快速煮沸法提取的DNA完全可以用于毛细管电泳荧光标记检测。

The extraction DNA is suitable for capillary electrophoresis with fluorescence detection system .

3种基于PCR的荧光标记方法的比较研究

Three fluorescent labeling methods based on PCR : a comparative evaluation

应用荧光标记多重PCR对原发性胃癌进行微卫星不稳定性检测

Detection of Microsatellite Instability in Primary Gastric Cancer Using Fluorescence-Labelled Primers Multiplex PCR

应用多重PCR进行微卫星荧光标记-半自动基因组扫描

Fluorescence based semi automated gene scan with microsatellite markers by multiplex PCR techniques

一种新的基因芯片检测荧光标记技术：通用引物U2联合标记法

A new fluorescent labeling technique in microarray studies : universal primer U_2 labeling

荧光标记mRNA差异显示技术

Fluorescent mRNA differential display technique

免疫磁性细胞系统分离筛选和荧光标记骨髓干细胞亚群Thy-1~（low）Lin-Sca-1~+的研究

The study of isolation and label of Thy-1 ~ ( low ) Lin ~ - Sca-1 ~ + bone marrow stem cell subset by MACS and CFDA SE

荧光标记mRNA差异显示技术在分离食管癌相关基因片段中的应用

Application of fluorescence label mRNA differential display technique to isolation of human esophageal cancer associated gene cDNA fragments

使用的实验方法为荧光标记后激光共聚焦成像、流式细胞仪检测和westernblot。

Western Blot , flow cytometry and fluorescently confocal imaging after fluorescent labeling were used in experiment . 5 .

目的：荧光标记物是目前杂交型基因芯片所检测的主要目标底物，本实验比较了3种基于PCR的荧光标记方法在基因芯片检测中的应用效果。

Objective : To compare the applied efficacy of three fluorescent labeling methods based on PCR in genechip detection .

间接免疫荧光标记流式细胞术检测移植瘤血管内皮生长因子（VEGF）荧光峰值的表达情况；

The expression of VEGF in xenograft tumor cells was analyzed by indirect immunofluorescence flow cytometry .

利用SSR荧光标记分析90个糯玉米地方品种的遗传多样性

Analysis of Genetic Diversity Among 90 Waxy Corn Landraces Using Fluorescent SSR Markers

在用测序仪对DNA测序前要对其进行复制和荧光标记。

For DNA , copies of the DNA to be sequenced are made and labeled with fluorescent markers before they are identified using a sequencing machine .

以NS组为对照并以GFP为荧光标记物，用流式细胞仪（flowCytometry，FCM）分析各实验组肝细胞的转染率。

TE of rat hepatocytes were analyzed by flow cytometry ( FCM ) using GFP as fluorescent marker .

间接免疫荧光标记病毒即刻早期抗原（IE）；

CMV immediately early antigen ( CMV IE ) in HF cells was analyzed by indirect immunofluorescence assay .

利用间接免疫荧光标记和Western印迹方法分析蛋白激酶C活性对锚蛋白及CD44的亚细胞分布及蛋白质表达的影响。

Effects of PKC activity on subcellular distribution and protein expression of ankyrin and CD44 was analyzed respectively with indirect immunofluorescence and Western blot .

方法分A、B、C3段扩增TEM基因，并对A、B、C3段扩增产物进行四色荧光标记全自动测序。

Methods Three fractions including A , B and C amplified from TEM gene were automatically sequenced with four-color fluorescence labelling .

多协议标签交换在光网络中的演进与应用利用荧光标记SSR技术鉴定花生F1代杂交种

Evolvement and Development of MPLS in Optical Network Identification of Peanut Hybrids Using SSR Marker with Fluorescence Labeled M13-tailed Primer

采用荧光标记半定量逆转录聚合酶链反应（RT-PCR）方法检测了凋亡相关基因sFas、Fas和Bcl-2的表达。

MRNA expressions of sFas 、 Fas and Bcl-2 genes were then measured with fluorescence-labeled semi-quantitative RT-PCR .

方法采用不对称PCR方法，扩增HLAA基因的第2,3外显子，荧光标记扩增产物，作为杂交模板。

Methed Unsymmetrical PCR was used to amplify HLA A gene exon 2,3 . The PCR products were used as templates for hybridization .

利用SSR荧光标记定位萍乡显性核不育水稻育性恢复基因

Mapping the Genes Involved in Fertility of Pingxiang Dominant Genic Male Sterile Rice by Using Fluorescent-labeled SSR Makers

为了评价免疫分型在急性白血病（AL）分型中的科学意义，应用形态学分型、直接免疫荧光标记流式细胞仪检测免疫分型对68例AL进行了分析。

To evaluate the significance of immunologic classification for typing of acute leukemia ( AL ) . 68 cases of AL were classified by morphologic and immunologic typings .

术后各时间点B、C组心脏、肺、肝、脾、肾组织均未见DiI荧光标记细胞。

Cells marked by DiI were not been founded in the tissue of the heart , lung , liver , spleen and kidney at each time point .

采用不对称PCR和间接荧光标记技术进行单链DNA扩增和荧光标记，标记样品与寡核苷酸芯片杂交后，进行芯片清洗、扫描及结果分析。

By means of asymmetric PCR and indirect fluorescent-labeling and technology to amplify and label the samples , they were hybridized with the oligonucleotide microarrays , followed by washing , scanning and analysis .

样品DNA通过PCR扩增、体外转录等技术掺入荧光标记分子，与微阵列杂交后，通过荧光扫描仪扫描及计算机分析即可获得样品中大量基因序列及表达信息。

After hybridization of the DNA chip with the labeled sample targets , high-throughput gene sequence and expression information can be retrieved by scanning of the DNA microarray and the analysis of computer .

量子点可作为荧光标记修饰在蛋白质、DNA、RNA等生物大分子上，从而实现分子的诊断、检测等分析具有重要的意义。

Quantum dots can be used as fluorescent modification in protein , DNA , RNA and other biological macromolecules , thus to achieve molecular diagnostic testing , analysis is of great significance .

二次谐波（SecondHarmonicGeneration，SHG）成像技术由于具有空间高分辨率、无需荧光标记，对生物样品的光损伤小，在介质中的穿透深度大等优点，在生物医学领域具有广阔的应用前景。

Second harmonic generation ( SHG ) imaging needs no fluorescent labels and brings no photobleaching or toxicity . It 's a promising tool for high-resolution , three-dimensional studies in biomedicine fields .

westernblot法、流式细胞仪间接免疫荧光标记法测定Aβ对BV-2细胞iNOS蛋白表达的影响，并应用免疫细胞化学方法对iNOS蛋白的表达情况进行观察。

INOS mRNA expression was detected by RT-PCR , iNOS protein expression was examined by Western Blot , flow cytometry and immunocytochemistry .