荧光偏振
- 网络polarization;Fluorescence Polarization;fpia
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结果表明,磁化水对荧光偏振度(P)及膜的微粘度均有显著的影响。
The results indicate that the magnetized water has significant influence upon the fluorescence polarization and membrane microviscosity .
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荧光偏振检测血清中HBV多聚酶基因突变
Detection of mutations of HBV polymerase gene in serum by fluorescence polarization
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测定心肌细胞线粒体内膜的荧光偏振度及血清丙二醛(MDA)含量。
The fluorescence polarization of myocardial mitochondrial membrane and the content of serum MDA were detected .
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基于荧光偏振的HPV病毒基因检测系统研究
Research of HPV gene detection system based on fluorescence polarization
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方法:用特异性荧光偏振免疫法测定CsA全血浓度,对406例患者监测2013例次。
METHOD : A total 2013 samples from 406 renal transplant recipients were measured by specific fluorescence polarization immunoassay .
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氟中毒导致淋巴细胞膜胆固醇含量增加,膜荧光偏振度P值和微粘滞度η值升高,表明氟能降低淋巴细胞膜的流动性。
Cholesterol content of lymphocytes membrane was increased , P value of membrane fluorescence polarization degree and η value of viscosity increased , the results showed that lymphocytes membrane fluidity could be decreased by fluorine . 7 .
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发现心肌和骨骼肌线粒体内膜荧光偏振值和微粘度均较安静时增高,示内膜流动性显著降低(均P<0.01);
The results showed that the fluorescence polarization and microviscosity values of IMM in rat heart and skeletal muscle were higher than the resting values ( p < 0.01 ), which meant the IMM fluidity decreased obviously .
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方法:以荧光偏振免疫法(FPIA)对194例癫痫患儿进行血药浓度监测。
Method : The serum concentrations of194 children with epilepsy was monitored by fluorescence polarization immunoassay ( FPIA ) .
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采用ATR-FTIR、荧光偏振和SEM对量子点的毒性机制进行了探讨。
The mechanism of cytotoxicity of QDs was also studied through ATR-FTIR spectra , FL polarization , and SEM .
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方法以荧光偏振免疫法(FPIA)检测一定时间下的血清和脑脊液中MTX的浓度。
METHODS The concentrations of MTX in serum and CSF were determined by FPIA .
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用荧光偏振、圆二色谱(CD)和准弹性光散射等实验技术研究了中药黄芪对人红细胞膜的作用。
Effect of Astragalus on human erythrocyte membranes ( HEM ) was studied by fluorescence polarization , circular dichroism ( CD ) and quasi-elastic light scattering ( QELS ) techniques .
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红细胞膜流动性测定采用荧光偏振法,应用四甲基偶氮唑盐(MTT)法培养大鼠外周血淋巴细胞并测定淋巴细胞转化率,观察大剂量维生素E对免疫细胞增殖活性的影响。
The method of fluorescence polarization was used to measure membrane fluidity and MTT was used to detected the lymphocyte transformation rate which reflected proliferation activity of immune cell .
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方法:利用荧光偏振免疫分析法(FPIA)对135例癫痫病人进行PB血药浓度监测,并对测定结果进行分析。
Methods : 135 epilepsy patients were monitored the PB blood concentrations by FPIA method and the result were analyzed .
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结果:HAPC患者红细胞膜荧光偏振度与对照组比较增加(P<0.01)。红细胞膜过氧化脂质与对照组比较增高(P<0.01)。
Results : The membrane fluorescence polarization degree of erythrocyte was significantly increased and the membrane fluidity of erythrocyte remarkably decreased ( P < 0.01 , P < 0.01 ) .
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本文采用DPH荧光偏振技术研究了五氧化二钒(V2O5)对大鼠肾皮质线粒体和微粒体膜脂流动性的影响。
The study has been done on effects of V_2O_5 to membrane fluidity of the renal cortical mitochondria and microsome of rat by using DPH fluorescence polarization technique .
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方法采用荧光偏振技术对36例急性脑梗死患者和24例正常对照组外周血PMN膜脂流动性进行了测定。
Mothod Fluorescence polarization technique was used to measure the degree of membrane fluidity of PMN in 36 patients with acute cerebral infarction .
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针对HPV病毒的检测难题,设计一种将荧光偏振技术用于病原微生物检测和基因分析的荧光偏振自动检测系统。
Considering the difficulties of HPV detection , an automatic detection system is designed with fluorescence polarization technology , which is used in pathogenic germs detections and gene analyses .
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DPH标记细胞膜的动力学与膜脂流动性的荧光偏振校正测量
Dynamics of labelling cell membrane with DPH and the corrected determination of membrane fluidity by fluorescence polarization
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目的对荧光偏振免疫法(FPIA)测定血浆同型半胱氨酸(HCY)的方法学进行初步评价。
Objective To evaluate the detection of plasma homocysteine ( HCY ) with fluorescence polarization immunoassay ( FPIA ) technology .
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方法用荧光偏振免疫法检测了新乡市100名正常人及50例冠心病患者HCY水平。
[ Methods ] The HCY level of 100 healthy people and 50 patients with coronary heart disease was tested with fluorescent polarization immunoassay .
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目的:建立快速测定茶碱血清浓度的高效液相色谱(HPLC)法,研究应用此法和常规的荧光偏振免疫分析(FPIA)法测定结果的相关性。
Aim : To establish a rapid HPLC method determining serum theophylline concentration and research the relativity of results determined by HPLC and by routine FPIA .
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采用荧光偏振免疫法(FPIA)测定了单用氨茶碱和氨茶碱与替硝唑合用时兔体内血清茶碱浓度,观察了替硝唑对茶碱药物动力学的影响。
Aminophylline in the serum was determined by FPIA having taken aminophylline and aminophylline combined with tinidazole .
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方法:选择同种异体犬肺移植术后存活的3个病例,用单克隆抗体-荧光偏振免疫法(MA-FPIA)测定其不同时期的CsA全血谷浓度。
Methods : We used MA FPIA to monitor the blood concentration of CsA for the 3 survival dogs with lung allograft transplantation .
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用荧光偏振法测定了36例急性脑梗塞患者红细胞膜微粘度,同时对血浆MDA、SOD含量进行了测定,并与24例正常人进行比较。
Fluorescence polarization method was used to measure the degree of erythrocytes membrane microviscosity in 36 patients with acute cerebral infarction , the plasma MDA and SOD were also measured , which compared with healthy persons .
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本文采用美国雅培公司产免疫荧光偏振分析仪,在4例成人癫痫中较详细地观察了PHT与VAL单用和合用,在不同时间中血清及唾液药物浓度。
Using TDX-fluorescence polarized analyser , Concentration of phenytoin valproate both in blood serum and in saliva were determined singly and in combination .
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MEGX浓度采用TDx分析仪测定,方法为荧光偏振免疫测定。
MEGX levels were measured with TDx analyzer .
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应用荧光偏振免疫法(FPIA)对274例肾移植术后患者行2312人次全血环孢素A(CsA)浓度测定。
Whole blood concentration of CsA was assayed 2312 times in 274 renal transplant recipients by the fluorescence polarization immunoassay ( FPIA , abbott TDx ) .
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红细胞膜流动性和红细胞变形性:红细胞膜的荧光偏振度(ρ)和微粘度(η),在饲喂洋葱后第1天就显著升高(p0.01);
The fluidity of erythrocyte membrane and the erythrocyte deformability : The fluorescence polarization parameter ( p ) and the microviscosity (η) of the erythrocyte membrane increased dramatically on day 1 post-feed onion ( p0.01 ) .
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方法选择136例急性脑梗死患者和70例对照,采用荧光偏振免疫分析法测定其空腹血浆Hcy浓度。
Methods Plasma homocysteine was determined by fluorescence polarization immunoassay in 136 patients with acute cerebral infarction and 70 controls . 15 patients with hyperhomocysteinemia received remedy of vitamin supplement .
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方法对126例癫痫患儿使用丙戊酸钠达稳态血药浓度后,采用荧光偏振免疫法(FPIA)测定其血药浓度,并分析结果。
Methods The drug serum concentration in126 epileptic cases was determined fluorescence polarization immunoassay ( FPIA ) . The results had been analyzed .