荧光检测

基于CCD器件的农药荧光检测系统的研究

Study on fluorescence detection system for pesticides based on charge-coupled devices

基因芯片CCD荧光检测及图像处理

The Gene - chip Fluorescence Detection by CCD and Image Processing

多重PCR和五色荧光检测在一起碎尸案中的应用

The application of mutiplex amplification and five fluorescent technique in a corpse-chopping case

建立了豫医无毛小鼠无毛基因的原位PCR荧光检测方法，并尝试用原位PCR的方法对其无毛基因进行了遗传定位的前期工作。

The method of PCR in situ for detection of hr gene is founded .

DNA芯片的CCD荧光检测

DNA chip fluorescence detection by CCD

建立了丹酚酸B的高效液相色谱-荧光检测含量测定方法。

A new method for the determination of Salvianolic acid B by high performance liquid chromatography with fluorescence detection has been established .

Ba离子储存信号的射频和荧光检测

RF and Fluorescence Detection of the Trapped Ions Ba

采用HPLC荧光检测法测定血清样品中的左羟丙哌嗪浓度。

A method of HPLC with fluorescence detection was established to determine the concentration of levodropropizine in serum .

人血液淋巴细胞膜LDL受体荧光检测的研究

A Fluorescene Detection of the Lymphocyte Membrane LDL-Receptor in Human Blood

采用液相色谱分离（HPLC）荧光检测快速测定儿童血浆中的16种氨基酸。

Amino acids in plasma of children were determined by high performance liquid chromatography ( HPLC ) and fluorescence detector .

集成光纤的PDMS夹心式微流控芯片及便携式LED诱导荧光检测系统

PDMS Sandwich Microfluidic Chip Integrated with Optical Fiber and Its Portable Analyzer with LED Induced Fluorescence Detection

HPLC荧光检测法测定SO2吸入后小鼠脑、心和肺亚硫酸盐水平

The measuration of sulfite levels in brains , hearts and lungs of the mice after sulfur dioxide inhalation by HPLC with fluorescence detection

猪乙脑病毒IgG抗体间接免疫荧光检测方法的建立与初步应用

Establishment of the indirect immunofluorescence assay for detecting the IgG antibody of epidemic encephalitis virus in serum of pigs and its preliminary use

目的使用液体培养基，建立一种分枝杆菌（Mycobacterium）菌种初步鉴定的非放射快速荧光检测法。

Objective To set up a method of Rapid Fluorimetry for identification of group of Mycobacterium by using the liquid culture medium .

对DNA序列分析方法，包括：毛细管电泳、阵列毛细管电泳、芯片毛细管电泳、超薄层毛细管电泳、质谱法、原子探针法、杂交法、流动式单分子荧光检测法，作了详细评述。

The analytical methods such as capillary electrophoresis , multiplexed array capillaries electrophoresis , integrated capillary electrophoresis chip , mass spectrometry , atom force microscopy , sequencing by hybridization and single molecular detection were commented .

Ⅱ型胶原免疫荧光检测表达阳性，RT-PCR检测Aggrecan、Ⅱ型胶原mRNA表达阳性。

Type ⅱ collagen expression was detected by immunofluorescence , mRNA expression of Aggrecan and collagen type ⅱ were detected by RT-PCR .

芯片毛细管电泳荧光检测PC12细胞中神经递质

Determination of Neurotransmitters in PC 12 Cells by Microchip Electrophoresis with Fluorescence Detection

目的建立了测定大鼠血浆中格帕杀星浓度的RPHPLC荧光检测法和研究格帕杀星在大鼠体内的药物力学。

Objective A sensitive , specific and rapid RP HPLC with fluorescence detection was developed and validated to determine grepafloxacin in rat plasma in order to study its pharmacokinetics in rats .

方法：采集东北地区100名受试者24h食物样品，采用HPLC可编程序荧光检测法，对14种PAHs进行定量分析。

Methods : The 24 h food samples were collected from 100 subjects in Northeast China and detected with HPLC . The quantitative analysis to 14 kinds of PAHs was performed .

免疫组织化学检测肾脏胶原Ⅳ（ColⅣ）和可溶性tTG表达。间接免疫荧光检测不溶性tTG分布。

Collagen ⅳ( Col ⅳ) and soluble tTG protein in kidney were examined by immunohistochemistry , insoluble tTG by immunofluorescence .

分别于术后1周、2周、1个月和2个月时取脑、冰冻切片，进行Nissl染色和BrdU免疫荧光检测。

After grafting for 1 week , 2 weeks , 1 month and 2 months , BrdU-labeled cells were detected by Nissl staining and BrdU immunofluorescence .

建立了毛细管电泳分离激光诱导荧光检测（CELIFD）分析分枝杆菌脱氧核糖核酸（DNA）限制性内切酶谱的新方法。

A new method for rapidly detecting restriction enzyme pattern of mycobacterium deoxyribonucleic acid ( DNA ) by capillary electrophoresis with laser induced fluorescence detection ( CE-LIFD ) was developed .

首先，我们利用荧光检测技术、分子生物学、细胞生物学技术建立了基于细胞功能的高通量筛选方法，筛选并鉴定了淫羊藿Epimediumbrevicornum中的类激素作用活性成分。

First , we developed cell-based sensitive fluorescent assay for high throughput screening and identified an active component isolated from Epimedium brevicornum .

结果：免疫荧光检测VEGF阳性的成肌细胞占85%～90%，ELISA法检测培养液中VEGF浓度平均为214ng/（106cell·24h）。

Results : Immunofluorescence detection showed that 85 % - 90 % myoblasts were VEGF-positive ; ELISA showed that the mean VEGF level was 214 ng / ( 10 ~ 6 cell · 24 h ) in the culture medium .

建立了测定格列喹酮血浆浓度的HPLC－荧光检测法，并对10名健康受试者随机交叉口服60mg格列喹酮胶囊和格列喹酮片后的药代动力学性质及其生物等效性进行了研究。

A HPLC method with fluorescence detection was developed and validated to determine gliquidone in human plasma , and the pharmacokinetics of two gliquidone formulations ( capsule and tablet ) were studied in ten healthy Chinese volunteers .

液相色谱-荧光检测法（LC-FLD）测定贝类样品中石房蛤毒素（STX）和decarbamoylsaxitoxin（dcSTX）。

Saxitoxin ( STX ) and decarbamoylsaxitoxin ( dcSTX ) were determined by liquid chromatography with fluorescence detection .

比较了三种基于SSR分子标记的指纹图谱构建方法，即琼脂糖凝胶电泳检测、TP-M13-SSR毛细管电泳荧光检测和SSR高分辨率熔解曲线分析检测的优劣，提出高效农作物品种鉴定和指纹图谱构建的策略。

The advantages and disadvantages of agarose gel electrophoresis , TP-M13-SSR capillary fluorescence detection and SSR high resolution melting curve analysis were compared . A suitable strategy for crop cultivar identification and fingerprinting was proposed .

hRI基因能够转染到脐血CD34+细胞上，转染效率达35%，westernblot和免疫荧光检测转染后CD34+细胞hRI基因有阳性表达。

HRI can be transfected on umbilical blood CD34 + cells , and the transfection efficiency was 35 % . The positive expression of hRI gene on transfected CD34 + cells is identified by Western blot and immune fluorescence assay .

HL一60与HBMEC作用24h后，经免疫荧光检测发现20一1由正常时连续的变为不连续的。提示HL一60细胞通过打开紧密连接进而穿过血脑屏障；

After the interaction of HL - 60 and HBMEC for 24h , ZO - 1 continuous expression was changed to be uncontinuous by fluorescence detecting , which suggested that HL - 60 cells crossed BBB by opening TJ .

第1、2块分别于第7d和14d时行MAP2免疫荧光检测；第3块于10d时行AChE组织化学染色。

The neurons differentiated from NSCs in first and second plate was detected by MAP-2 immunofluorescence respectively at 7,14 day and the neurons in third plate were detected by AChE histochemistry at 10 day of culture .