反相色谱
- 网络Reversed Phase Chromatography;reverse-phase chromatography;rplc;RPC
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可行性验证方法是通过装填反相色谱填料包括C18填料和苯基填料,并对一些中性化合物进行分离。
The feasibility was verified by reversed phase chromatography including C18 and phenyl column , which were used to separate some neutral compounds .
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液相二极管检测器联用(HPLC-DAD)建立控制pH的反相色谱法、双离子对色谱法及离子交换色谱法的色谱条件。
The analytical condition of reversed phase chromatography , binary ion pair chromatography and ion - exchange chromatography were built by the combination of HPLC with Photodiode Array Detector ( HPLC-DAD ) technology .
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方法应用正相和反相色谱包括半制备HPLC方法分离和纯化化合物;
Methods Column chromatography in association with semi-preparative HPLC were used for the isolation of compounds .
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PAD在反相色谱法测定聚合物有关热力学性质的实验数据处理中的应用
The Application of PAD in Processing Data of IGC Experiment to Determine Some Thermodynamic Properties of Polymers
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实验以反相色谱柱为分离柱,采用紫外检测方法,考察了检测波长、离子液体烷基链长度、离子液体溶液的浓度以及pH值等对分离和测定的影响。
The effects of the detection wavelength , pH value , length of alkyl chain on different ionic liquids , and the concentrations on the separation and determination of these analytes were performed .
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A30菌株培养液经硫酸铵沉淀、FPLC疏水色谱、HPLC反相色谱后,纯化得到一种拮抗活性物质,命名为P1。
An antagonistic substance produced by A30 was purified by ammonium sulphate precipitation , FPLC and HPLC and named P1 .
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以常压层析系统纯化的碱性脂肪酶为出发蛋白,采用高效反相色谱对其进一步分离纯化,达到N末端氨基酸测序及肽谱分析所需的纯度。
In order to satisfy the needs of purity of the N terminal amino acid sequence determination and peptide analysis , an alkaline lipase purified with low pressure chromatography system was further purified by high pressure reversed phase chromatography .
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方法:HPLC法;采用C18V反相色谱柱,二元梯度洗脱,二极管阵列检测器,以芍药苷为内标作为相对标准。
Methods : The HPLC method was used , chromatography conditions were C 18V column , binary solvent-delivery gradient elution , diode-array detector , internal standard ( paeoniflorin ) were taken as the criteria for quality control .
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采用C18反相色谱柱,利用在流动相中加入手性选择剂的方法实现酮康唑对映体的拆分。
A simple and effective method for the separation of ketoconazole enantiomers was developed using the chiral mobile phase additive on a C18 reversed-phase column .
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用反相色谱法、电子天平法和ESCA对氧化前后炭纤维的表面性能作了研究。
The surface properties of carbon fibres , which were oxidized or not , were studied by Inverse Gas Chromatography , electrobalance , and ESCA .
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反相色谱中LgI作为一个新的药物疏水性表征参数
The Parameter LgI in Reversed Phase Liquid Chromatography as a New Characterization of Drug Hydrophobicity
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该苦味部分经LunaC18反相色谱进一步纯化并经感官鉴评,获得三个苦感最强的苦味肽(p2,p4,p8);
Subsequently , the bitter fractions recognized were separated by reversed phase HPLC on LUNA C18 column and confirmed by sensory testing , three peptides with the most bitter flavor ( p2 , p4 , p8 ) were obtained .
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结果表明,这种C18-MPS-VTMS硅胶杂化整体柱可运用于反相色谱模式以及离子交换模式。
The resulting C18-MPS-VTMS silica hybrid monolithic column can be used in different separation modes , including reversed phase mode and ion exchange mode . 2 .
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方法采用高效液相色谱-紫外检测法(HPLC-UV),以卡马西平为内标,菟丝子灌胃给药后,大鼠含药血清样品经液-液萃取后在C18反相色谱柱上进行分离测定。
Methods The quick and sensitive HPLC-UV method was established with carbamazepine acting as internal standard . After the rats were douched with dodder seed , drug-containing serum samples of rats were separated and measured on C18 reverse-phase chromatography column after being liquid to liquid extracted .
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反相色谱法在聚合物研究中的应用
The application of inverse gas chromatography in the study of polymer
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PICO-TAG反相色谱法测定鸡蛋中氨基酸含量
Reversed-Phase High Performance Liquid Chromatographic PICO-TAG Analysis of Amino Acids in Eggs
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反相色谱法研究聚醚的溶解度参数
Study on solubility parameters of polyether by inverse gas chromatography
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正相色谱是吸附色谱,而反相色谱主要是分离色谱。
TLC is adsorption chromatography , and RP-TLC is mainly separation chromatography .
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羟基酸的离子相互作用间接光度反相色谱研究
Studies On Ion-Interaction Indirect Photometric Reversed-Phase Chromatography of Hydroxy Acids
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反相色谱在重组多肽精细纯化中的应用
Application of reverse phase chromatography in recombinant polypeptide polishing purification
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局部麻醉药的反相色谱安培法检测
Liquid Chromatography of Local Anaesthetics by Amperometric Detection
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反相色谱在高聚物测试中的应用
Application of Anti-phase Chromatography in Detection of Polymers
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TBP-(X-5)反相色谱分离m-NPF分光光度法测定铀
M-npf-spectrophotometric determination of uranium after separation by tbp - ( x-5 ) reversed phase chromatography
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本地芦笋中芦丁的提取及其反相色谱鉴定
Determination and extraction of rutin from asparagus
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用于碱性物质分离的酰胺型反相色谱键合相的制备及评价
Preparation and Evaluation of Amide-Octyl-Bonded Phase for the Separation of Basic Substances in Reversed-Phase Liquid Chromatography
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结果表明该固定相具有反相色谱性能,并具有特殊的分离选择性。
The result shows that the stationary phase has reversed-phase chromatographic performance and especial separation selectivity .
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聚二乙烯基苯微球制备及其在小分子和多肽反相色谱分离中应用
Preparation of Uniform Polydivinylbenzene Microspheres and Their Application in Reversed-phase Chromatographic Separation of Small Molecular Compounds and Peptides
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牛脾提取物相对分子质量分布及反相色谱图的研究与比较
Study and Comparison on the Molecular Distribution and Reversed Phase Chromatogram of Bovine Spleen Extract with HPLC Method
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反相色谱法研究人粒细胞集落刺激因子的离子交换色谱复性和稀释复性
Study on Dilution and Ion Exchange Chromatographic Refolding of Recombinant Human Granulocyte Colony-stimulating Factor by Reversed Phase Chromatography
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固体表面自由能及其分量的计算方法(Ⅱ)&反相色谱法和薄层毛细法
Calculation method of the free energy and its component on solid surface (ⅱ)── back gas chromatography and layer chromatography