酶活性测定

比较了这两种试验的结果。使用荧光筛选试验和酶活性测定研究1159例新生儿脐血标本，分别有26例（2.2%）及24例（2.1%）PK活性低于正常水平。

The results of these two tests were compared , of 1 159 infant cord blood samples studied , 26 and 24 samples ( of 2.2 and 2.1 % ) had abnormally low levels of pk activity , using the screening test and enzyme assay , respectively .

为了建立体外筛选RmlC酶抑制剂的分子模型，我们需要获得高纯度的RmlC酶蛋白，并为建立快速、精确的酶活性测定方法和研究酶促反应动力学特性提供物质保障。

In order to establish a molecular model based on RmlC enzyme assay to screen inhibitors of RmlC , a large amount of RmlC protein must be acquired .

基于分子信标的尿嘧啶DNA糖苷酶活性测定

Assaying Uracil-DNA Glycosylase Using Molecular Beacon

W/O型微乳液中超氧化物阴离子的产生&微环境中SOD酶活性测定方法研究

Generation of superoxide anion in reverse microemulsion ─ methodical study on measurement of SOD activity

吖啶酯作为一种高效的化学发光试剂，在临床免疫分析，DNA分析，生物酶活性测定等方面有着广泛的应用。

As a kind of highly efficient chemiluminescence reagent , Acridine esters are commonly used in clinical immunoassays and biological activity determination of enzymes .

猪囊尾蚴囊液抗原制备及ELISA检测条件的优化猪带绦虫囊尾蚴囊液谷胱甘肽S转换酶活性测定

Prep of Cysticercus cellulosae Cyst fluid antigen and optimization of ELISA Detection of glutathione S-transferase in Cysticercus cellulosae

细胞保护酶活性测定表明，SOD和POD酶活性随盐浓度的升高而升高；

The measurement on cell pro-tective enzymes showed that SOD and POD activities increased with the increase of salt concentra-tions ;

酶活性测定表明，除了果肉外的其它器官中都有芥子酶活性，这与RT-PCR的结果是一致的。

Myrosinase activity was detected in all the tested organs except for pulp , which is consistent with the result of RT-PCR .

本文对49例进行性肌营养不良症（PMD）中的DMD、LG、FSH三型进行了血清CPK、LDH、GOT及GPT四种酶活性测定与分析。

The activity of serum CPK , LDH , GOT and GPT was determined in 49 case of progressive muscular dystrophy ( PMD ) .

通过酶活性测定和紫外-可见光谱研究了胱氨酸对烟草多酚氧化酶（简称PPO）活性的影响。

The effect of cystine on the activity of polyphenol oxidase from Nicotina Tobacco has been studied by using enzymatic activity assay and UV-VIS spectroscopy .

β-半乳糖苷酶活性测定结果表明：nirS的表达受氧的抑制，在厌氧条件下的表达活性明显高于好氧条件。

The result showed that the expression of nirS was repressed by oxygen , and denitrification activity under anaerobic conditions was higher .

光照反应时间对SOD酶活性测定的影响较大，确定为25min较为适宜。

Illumination reaction time has significant effect on assay of SOD activity , 25 min is relatively suitable time required .

本文通过酶活性测定，荧光光谱和紫外光谱研究了外加Cu2+与烟草多酚氧化酶（简称PPO）的相互作用。

The interaction between Cu2 + and PPO ( polyphenol oxidase ) has been studied by employing enzymatic activity assay , fluorescence spectroscopy and UV / Visible spectroscopy .

[方法]大鼠染毒12周，分别在第3、6、9和12周处死动物，DNA损伤采用单细胞凝胶电泳（彗星试验）法，代谢酶活性测定采用分光光度法。

VC was given to rats by in injection 3 times a week for 12 weeks , and rats were killed at the 3rd , 6th , 9th and 12th week . DNA damage was detected by single cell gel electrophoresis ( comet assay ) .

利用病毒诱导的基因沉默技术在抗病材料上创造了目标基因的沉默，定量PCR和酶活性测定均证明目标基因表达量都有不同程度的明显下降。

The method of virus-induced gene silencing ( VIGS ) was used to silence target genes in the resistant line . Quantitative PCR and enzyme activity assay demonstrated that the expression of target genes decreased to different degrees in gene-silenced plants .

结论1.建立了以底物荧光法为主的外周血白细胞六种常见MPS酶活性测定方法，具有良好的稳定性、特异性和敏感性，操作相对简单、快速，适合临床推广应用。

Conclusions : 1 . We established the fluorescence substrate assays for measuring the six common MPS enzymatic activity in leukocytes , with good stability , specificity and suitable for clinical application . 2 .

通过免疫印迹，酶活性测定等方法在脑片水平研究维生素E（VE）对花萼海绵诱癌素（CA）诱导的骨架蛋白tau过度磷酸化的保护作用及机制。

To study the protective effect of vitamin E ( VE ) on hyperphosphorylation of cytoskeleton tau in neurons induced by calyculin A ( CA ) and the mechanism , VE or VE plus CA was administered to the cultured slices .

方法：以人白血病细胞株HL-60为研究对象，采用形态学观察，流式细胞仪、Caspase-3酶活性测定、免疫组化等方法。

Methods : HL 60 cell lines were used to explore the effects of pangolin extracts on the changes of the by observing the morphology followed by the flow cytometry , caspase 3 measurements and immunohistochemistry .

Arthrobacterglobiformis酯酶基因的克隆、表达和酶活性测定

Cloning and Expression of the Esterase Gene of Arthrobacter globiformis and the Activity Assay of the Esterase

在干旱胁迫处理条件下，在转基因植株的叶片和根部都发现GUS基因的表达，从GUS酶活性测定结果表明启动子都具有较强的表达活性，在干旱胁迫诱导后表达活性得到提高。

Under drought stress , gene expressions of GUS were found in leaves and roots of transgenic plants , GUS activity showed that the two promoters had a stronger expression of activity after drought stress-induced .

对象与方法先证者，女，新生儿期起病，9个月时经外周血白细胞β-半乳糖苷酶活性测定确诊为GM1神经节苷脂沉积病。

Methods The proband was a girl with psychomotor disorder from neonatal period . β - galactosidase activity of peripheral blood leukocytes were measured when she was 9 months old .

酶活性测定实验表明在蚯蚓CaMBPs中有Ca2＋－ATPase活性，但无NAD激酶活性。

The assay of enzyme activity indicates that there was CaM dependent Ca 2 + ATPase activity , but no NAD kinase one .

经固氮酶活性测定，PA19、PA2和PK1菌株具有较强的固氮酶活性。

The results showed that PA19 , PA2 and PK1 strains had high nitrogenase activities .

其中以蛋白质为底物时的酶活性测定方法方便快捷，但是当酵母蛋白酶A酶活性较低时，产物采用紫外吸收法以及Lowry法的检测灵敏度都比较低。

Three detecting methods in yeast proteinase A ( PrA ) assay including UV absorbance , Lowry method and modified Bradford method were compared when using casein or hemoglobin as substrates .

用组织印迹法、Northern分子杂交、DEAE-纤维素柱层析技术、酶活性测定以及抑制剂等研究了小麦幼苗各器官谷氨酰胺合成酶（GS）活性的变化和光对GS活性及GS-mRNA的影响。

The activityes of GS from the different organs of wheat seedlings and influence of light on GS and GS-mRNA have been studied by the employment of TPH , Northern blot , DEAE-celluose columns chromatography , enzyme activity assay and inhibitor .

采用BIOLOG碳素利用法、磷脂脂肪酸（PLFA）法和土壤酶活性测定等方法比较了三种水分条件（淹育、淹育晾干、非淹育）对水稻土微生物群落多样性及活性的影响。

Influence of soil moisture regime ( non-flooding , flooding-drying , and flooding ) on microbial community diversity and activity was investigated by determining Biolog sole carbon source utilization pattern , phospholipid fatty acid ( PLFA ) profiles and enzyme activity indices .

酶活性测定时，样品用双蒸水溶解，吸取样品溶液，与TMB和H2O2混和后于700nm处测定反应反应进程曲线，以含有等量新配制HRP的反应体系为对照，计算酶的相对活性。

For enzymatic assay , samples were dissolved in distilled water and aliquots of solution were mixed with TMB and H2O2 . Kinetics of enzymatic reaction was recorded at 700 nm and HRP activity was calculated in respect to the kinetic curve using fresh-prepared HRP as a control . 3 .

根据糖脎的物质吸收图谱、线性关系、最小检出限等方面都证明糖脎硫酸显色法是一种比较理想的DAHPS的酶活性测定方法，并确定此法为测定酶活性的第三种方法。

It was proved that the osazone sulfuric acid development method ( OSADM ) is a quite ideal DAHPS activity assay method , according to the osazone 's absorption map , the linear relations , detecting limit .

肝病患者的血清过氧化氢酶活性测定初探

Determination of the Serum Catalase Activity in Patients with Liver Diseases

发色多肽用于前激肽释放酶活性测定的研究

Research on Chromogenic Peptide in Determination of Prekallikrein / kallikrein Activity