厌氧培养

D毒素基因方法与厌氧培养法的一致性为100%。

The consistency between the method of PCR and anaerobic culture was 100 % .

采用厌氧培养技术，从厌氧活性污泥中分离得到一株产氢发酵细菌。

Anaerobic culture technique was adopted in this study to isolate a hydrogen producing bacteria in anaerobic activated sludge .

取盲肠内粪便作厌氧培养及细菌种群DNA指纹图谱分析。

The feces in cecum were cultured for anaerobic bacterial growth and analysed by bacterial group DNA fingerprint spectrum .

方法在厌氧培养条件下培养各株变形链球菌，用OD值测量法计算粘附量。

Methods The adherence amount was measured by OD reading after anaerobic incubation .

将液体培养物稀释到同一梯度，定量接种于固体培养基上（绵羊血琼脂平板和特殊培养基），分别进行需氧和厌氧培养24~48h。

Things of the above cultured , after diluted to the same gradient and inoculated on solid culture mediums quantitatively , were cultured for 24 ~ 48 h under the condition with oxygen and without oxygen .

高温高压灭菌后根管内接种粪肠球菌Enterococcusfaecalis（ATCC29212）悬浊液，厌氧培养21天，得到感染根管的模型。

After preparation and sterilization . the specimens were inoculated with Enterococcus faecalis ( ATCC 29212 ) and incubated for 21 days .

反硝化还原酶活性和合成过程受O2的抑制，厌氧培养时，需要一定时间消耗系统中残余的O2来解除这种抑制作用。

The activities and synthesis of reduction enzymes are inhibited by oxygen . It takes some time to consume the O 2 left in an anaerobic incubation to release this inhibition .

加入水稻土中的含铬（VI）矿渣能够在厌氧培养条件下迅速发生还原转化，并随着体系中易还原氧化铁数量的增加，其还原速率加快。

Adding the chrome slag can be reduced rapidly in paddy soil with anaerobic incubation and the rate of Cr ( VI ) reduction promote with adding Fe ( III ) in the system .

方法对77例肝移植和1例未行肝移植的肝硬化患者进行术中采样，取外周血、门脉血及肠系膜淋巴结（MLN），分别进行需氧及厌氧培养，了解BT的发生率。

Methods Mesenteric lymph nodes ( MLN ), portal vein blood , and peripheral blood were collected during the liver transplantation for microbiological culture from 78 patients with cirrhosis .

实验结果表明，费氏丙酸杆菌菌株HZ-P-35在改良MRS培养基中厌氧培养时生长较为缓慢，96h左右对数生长期结束；

The following are the results : Strain HZ-P-35 has a slow growth in a modified MRS medium with a 96h time course of logarithmic growth phase .

方法：体外厌氧培养变形链球菌，用不同浓度抗龋IgY和IgY牙膏处理菌细胞，并对培养出的菌细胞做透射电镜和扫描电镜观察。

METHODS : Cultured Streptococcus mutans treated with different concentrations of anticarious IgY and IgY toothpaste was observed by transmission electron microscope ( TEM ) and scaning electron microscope ( SEM ) .

结果表明，最佳培养条件为25℃恒温厌氧培养24h且培养基初始pH值为5.5；通过单因素确定最佳碳源、氮源、刺激因子后采用三元二次回归正交旋转设计进行优化。

And the optimum incubation time was 24h , the optimum temperature was 25 ° C , the optimum broth initial pH was 5.5 . The optimum carbon source , nitrogen source and irritant factor were defined by single element , then optimized by CCRD .

以酵母自溶物为主要选择分离培养基，利用厌氧培养分离法从自然发酵的酸菜汁中分离出发酵酵母自溶物产乳酸能力强，并且产酸迅速的菌株A4；

With yeast extract as a elective medium , using anaerobic cultivation , a strain , A4 was separated from juice of nature sour pickle which could produce lactic acid more and quickly and was identified as Lactobacillus Plantarum .

方法：将发酵乳杆菌加入含不同浓度CPP-ACP的蔗糖培养基中，厌氧培养48h，用精密pH计检测培养基上清液的pH值，计算pH的变化值ΔpH（初始pH值-终末pH值）。

METHODS : Lactobacillus fermentum was inoculated in medium containing 1 % sucrose and different concentrations of CPP-ACP and grew in anaerobic condition at 37 ℃ for 48 hours . The primary pH values and final pH values of the supernate were measured , then the △ pH was compared .

结果：口腔细菌在牛心脑浸液（BHI）培养液中经48h厌氧培养，上清液在pH17的酸度下用乙醚提取可得到理想的色谱分析结果。

Results : Oral bacteria cultured in brain heart infusion broth ( BHI ); their supernatant was adjusted to pH 1 7 , and then extracted by ether . With the above conditions , an optimal result can be obtained by analyzing using HPLC .

通过多次培养试验，研制成功了新的厌氧培养基。

The new anaerobic medium was developed successfully through cultivation trials .

单纯化脓性胆囊炎手术标本厌氧培养结果32例分析

Results of 32 Operative Specimens Cultured Anaerobically from Simple Pyogenic Cholecystitis

利用这些厌氧培养技术，已成功分离培养了550余厌氧菌株系。

550 bacterium strains were isolated by the anaerobic operation .

培养区：主要有一般培养箱、恒温恒湿培养箱、厌氧培养箱等。

Inoculation area : the main equipment here is bio safety cabinet .

厌氧培养皿及厌氧培养管的临床应用&对传统的厌氧菌分离培养及鉴定方法的简化

Chinical Application of the Anaerobic Culture plates and Tubes

也可以通过厌氧培养箱来控制神经元培养环境中的氧浓度来模拟氧剥夺。

Anaerobic incubator was used to control the oxygen concentration to mimic oxygen deprivation .

几种厌氧培养基效能研究

Research on the efficacy of some anaerobic media

结果表明，厌氧培养条件下不同土壤中有效磷的释放不同。

The results showed that the release of available phosphate was different in different slurries .

用厌氧培养的方法从酸泡菜中分离出一菌株。

With the method of anti oxygen , a strain was isolated from pickled vegetable .

厌氧培养的水稻土中磷酸铁及磷的化学行为

Chemical Action of Ferric Phosphate and Phosphorus in Flooded Paddy Soil under Anaerobic Incubation Condition

在体外厌氧培养条件下，多山梨醇酯80明显促进棉花秸秆在混合绵羊瘤胃液中的降解。

In present research , polysorbate 80 could greatly stimulate the degradation of cotton-straw in anaerobic fermentation in vitro .

方法：对牙髓腔、牙周袋内的细菌进行需氧，厌氧培养，并用自动微生物鉴定系统对其进行分类鉴定。

Methods : The bacteria was cultured aerobically and anaerobically and identified by auto-microbial identification system ( AME ) .

方法在化脓性胆囊炎患者术中，用注射器于患处部无菌抽取标本，而后做需氧和厌氧培养。

Methods Specimens were withdrawed aseptically with syringe from affected parts of pyogenic cholecystitis then were cultured aerobically and anaerobically .

方法：采用透射电镜、厌氧培养等方法查找并确定致病菌，进行组织病理学和实验室检查及死后尸体解剖。

Methods : The anaerobic culture was performed for the tissue and the isolated bacteria were identified by electronic microscopy .

总结三年半来对住院患儿多种临床感染标本的厌氧培养，提高病原菌的检出率。

The pathogens of anaerobic culture of different kinds of clinical samples were detected from hospital children for three and a half years .