功能克隆

基因狩猎：功能克隆、定位克隆和表型克隆

Gene Hunting : Functional Cloning , Positional Cloning and Phenotype Cloning

目的：研制抗人喉癌/抗血管内皮因子（VEGF）双功能克隆抗体，用于喉癌抗血管生成治疗。

Objective : To study preparation of an anti-human laryngeal carcinoma and anti-VEGF bifunctional clonal antibody , applied to cure antiangiogenesis of laryngeal carcinoma .

除了我们描述的使用IBM云功能克隆持久性存储单元的方法之外，再无其他途径可以访问未附加的持久性存储单元。

There is no means to access an unattached persistent storage unit other than the method we described , that of cloning a persistent storage unit using the IBM Cloud capability .

目的为了研究人黑素瘤MIA/CD-RAP基因的生物学功能，克隆MIA/CD-RAP的cDNA，构建表达载体后，对其进行酶切和DNA测序鉴定。

Objective To study the functional role of MIA / CD-RAP in melanoma , MIA / CD-RAP cDNA was cloned and authenticated .

目的：为探讨研究未知基因CGI-100的功能，克隆人CGI-100基因并构建其真核表达载体。

Objective : To clone human CGI-100 gene and reconstruct its eukaryotic expressive vector for further investigation .

结论：成功构建了血管基膜衍生多功能肽克隆和原核表达载体，并对其进行了空间结构分析。

Conclusion : The cloning and prokaryotic expression vector was successfully constructed .

茶树重要功能基因克隆研究进展

Progress in Functional Gene Cloning of Camellia sinensis

新疆特殊生物功能基因克隆及应用研究

Studies on Molecular Cloning of Functional Genes from Special Organisms in Xinjiang and Their Application

创制突变体是作物功能基因克隆和品种选育的重要途径。

Mutants play an important role in cloning of functional genes in crops and crop breeding .

球孢白僵菌抗逆相关功能基因克隆与基因工程菌株构建研究

Studies on Cloning of Stress-tolerance Relative Genes from Beauveria Bassiana and Construction of Genetic Engineering Strains

目的为实现人μ型阿片受体在哺乳动物细胞中的表达以及研究其生物学特性、下游基因表达及功能研究，克隆了人全长μ型受体cDNA。

Objective : To study the biological activities , expression and function of downstream related genes in mammary culture cells , human μ opioid receptor cDNA is cloned .

hTRT催化功能区基因克隆及其在肿瘤中的表达

Molecular cloning of hTRT catalytic domain and its expression in tumors

基因陷阱是功能性基因克隆的一种有效方法，经典的基因陷阱以geo作为报告基因，对所克隆的基因类型没有选择性。

Gene trap is a powerful tool for functional gene identification and characterization . Classical gene trap employs geo as the reporter , and gives no discrimination on the types of trapped genes .

弓形虫SAG1基因功能片段的克隆、表达与抗原性分析

Cloning , expression and antigenicity analysis of gene encoding the major surface antigen 1 function of Toxoplasma gondii

拟南芥中三个未知功能基因的克隆和分析

Cloning and Analysis of Three Function Unknown Genes in Arabidopsis Thaliana

亚洲璃眼蜱唾液腺一新功能基因的克隆与测序

Cloning and sequencing of a tick salivary function gene in Hyalomma asiaticum

大豆启动功能片段的克隆及在转化甘草中启动β-葡糖苷酸酶基因的表达

Cloning of Soybean promoter Fragments and Expression in Transformed Glycyrrhiza uralensis Fisch

黑胸大蠊浓核病毒磷脂酶A2功能区的克隆及其表达

Cloning and Expression of Phospholipase A_2 Domain of Periplaneta fuliginosa Densovirus in E.coli

血管基膜衍生多功能肽基因克隆、表达及空间构象分析

Cloning , Expression and Space Conformation Analysis of Vascular Basement Membrane-Derived Multifunctional Peptide

目的从海洋生物双齿围沙蚕消化道上皮细胞中获得编码丝氨酸内肽酶某一功能基因的克隆。

Objective To obtain sequence encoding serine protease from clamworm digestive tract epithelial cells .

一个水稻功能基因的克隆和功能分析

Cloning and function analysis of a rice gene

人类未知功能蛋白质的克隆、表达、纯化和结晶

Parallel Cloning , Expression , Purification , Crystallization of Human Proteins for Structural Genomics

P-选择素凝集素-EGF功能域的克隆、表达及其单克隆抗体制备

Cloning , Expression of the Lectin-EGF Domain of P-Selectin , and Preparation of Its Monoclonal Antibody

转座子标签法可以用于突变体库的构建及功能基因的克隆，是功能基因组学研究的重要手段。

Transposon tagging is a powerful molecular bio-technique for the construction of mutant pool and the study of gene function .

小麦基因组庞大，重复序列多，重要功能基因的克隆存在很大困难。

Because of the huge genome and high ratio of repeat sequences in hexaploid wheat ( Triticum aestivum ), identifying and cloning of genes with important functions from wheat meet many difficulties .

经分析了解到某些酶和蛋白质与咖啡碱、氨基酸等多种有效成分的代谢途径相关，这为今后茶树功能基因分离克隆和功能基因组学研究奠定了基础。

Through analyzing , partial proteins were involved in the metabolic pathways of caffeine , amino acid and some other effective components . This study laid a foundation for gene cloning and functional genomic research of tea plant .

本文综述了近年来香蕉基因工程研究的最新进展，包括香蕉遗传转化受体系统的建立，香蕉作物中一些有益功能基因的克隆以及香蕉遗传转化的最新科研成果。

The paper summarizes the latest research advances in genetic engineering of banana having appearing in recent years , which include the establishment of the recipient systems of banana genetic transformation and the latest research achievements in the cloning and transformation of some advantageous functional genes in banana .

乙酰辅酶A羧化酶CT功能域基因的克隆与表达

Clone and Expression of Acetyl CoA Carboxylase CT Domain

将上述定位的鸡的20个功能基因的BAC克隆分别与利用胚胎成纤维细胞制备的鸭和鸵鸟的中期染色体杂交。

The above BAC clones of20 chicken genes were hybridized by FISH with duck and ostrich metaphase chromosomes prepared from embryo fibroblasts .

IBD泛指一系列的肠功能失调，如克隆病及溃疡性结肠炎。

Inflammatory bowel disease ( IBD ) is an umbrella term that includes a variety of intestinal disorders , such as Crohn 's disease and ulcerative colitis .