功能基因

目的通过建立华支睾吸虫成虫cDNA文库，筛选其功能基因，以研制华支睾吸虫的药物靶标。

Objective To screen functional gene through construction of the cDNA library of adult Clonorchis sinensis .

DNA损伤修复与基因组稳定性密切相关，因此我们的工作，为研究肿瘤癌变机制与开发具有临床应用价值的功能基因提供了重要的实验依据。

DNA damage leads to the genome instability . So our work will help to elucidate the mechanisms in carcinogenesis and to develop clinical-based functional gene therapy .

DNA插入突变是标记、分离植物病原真菌功能基因的有效途径之一。

DNA insertional mutagenesis is one of the most effective ways to identify pathogenicity genes of phytopathogenic fungi .

野家杂交猪F1代群体8个繁殖功能基因的多态性分析

Research on the Polymorphism of Eight Reproduction Genes in F_1 Generation of Hybrid Pig

对5个未知功能基因进行RT-PCR和实时荧光PCR方法验证，结果与基因芯片结果相一致。

The genes of unknown function were verified by RT-PCR results and RT-qPCR results .

利用水稻功能基因SSR标记鉴定水稻种质资源

Evaluation of Rice Germplasm Using SSR Markers of Functional Gene in Rice

破色期番茄果实均一化cDNA沉默文库的构建和功能基因筛选模型的初步建立

Construction of a Normalized cDNA Silencing Library of Tomato Fruit and Model Establishment of Screening Specific Functions of Genes

在众多丝状真菌的研究工作中，功能基因的研究是一个重要的方面，功能基因的研究方法有很多种，比如基因敲除技术，转座子标签法，RNA干扰等。

There are many methods to study functional genes , such as gene knockout , transposon tagging and RNA interference , et al .

这一结果为今后研究L1降解精喹禾灵的机理、功能基因等奠定了基础。

This result provided a basis for future research on degradation mechanism and functional genes .

结论SSH技术是筛选新的功能基因的有效方法；

Conclusion Suppression subtractive hybridization ( SSH ) is an effective method for screening new function genes .

抗人膀胱癌/抗VEGF双功能基因抗体对血管内皮细胞增殖的影响

Effect of bispecific antibody ( Bs Ab , anti-human bladder cancer / anti-VEGF ) on the proliferation of vascular endothelial cells in vitro

研究结果为今后利用鱼类基因组数据库和生物信息学快速鉴定新的鱼类功能基因打下了方法学基础，也为进一步开展鱼类IL-2基因功能研究提供了科学依据。

This report provided a quick method to identify new functional genes of fish through utilizing fish genomic databases and bioinformatics .

BLAST注释结果表明，具有已知或推测功能基因270个，未知功能基因258个，未比对上的基因182个（可能是新基因）。

The results of BLAST annotation showed : 270 known or putative functional gene , 258 unknown gene , 182 no hits ( maybe new genes ) .

结论：人工Mu转座技术是研究芽孢杆菌功能基因的快速有效的好方法。

Conclusion : Artificial Mu transposition complexes technology was a rapid and efficient tool for studying functional genes in Bacillus .

通过对24个样品的基因组DNA提取和功能基因芯片技术分析后，发现氮肥的施用对土壤中微生物群落结构和植物生长产生了显著的影响。

Soil microbial genomic DNA in 24 samples were extracted and analyzed with FGAs . The results showed that adding nitrogenous fertilizer would influence the structures of microbial community and plants in the samples .

由于这样的分子标记实际上就是功能基因本身局部片断的扩增结果，所以能非常可靠地直接鉴别该功能基因，F2群体中分子标记的分离比例与2对基因的独立分配比例完全一致。

In F-2 population the segregation of molecular markers were in consistent with expected ratio of two independent genes .

目的探讨抗人膀胱癌/抗CD3双功能基因抗体对LAK细胞增殖和增强细胞毒的作用。

Objective To study effect of BsAb ( anti-bladder cancer / anti-CD3 ) on proliferation and cytotoxicity of LAK cells .

对GUS表达进行了仔细研究，发现了许多特殊表达模式，为功能基因研究提供了重要线索。

The patterns of GUS expression were further studied . The results obtained were important clues for functional genes research .

同微阵列等分子生物技术一起，同步PCR技术将会在功能基因解析和病害分子诊断等方面发挥重要作用。

Together with molecular bio_techniques like microarray , real_time PCR will play a very important role in many aspects of molecular life science such as functional gene analysis and disease molecular diagnostics .

植物花器官的发育是由器官特异性基因决定的，这些基因包括ABC模型的A、B、C功能基因，同时还有决定胚珠发育的D功能基因和E功能基因。

Floral organ development is controlled by organ identity genes , including A - , B - , C-function genes of ABC model and SEP genes . Moreover ovules development is determined by D genes .

另一Vκ和VH具备鼠抗体可变区特征，为抗体功能基因。

The other VH and V κ are functional genes , for they are highly homologous to the variable region genes of murine immunoglobulin .

本研究的目的在于通过构建中华卵索线虫cDNA文库来保存该物种基因资源，便于相关功能基因的筛选和研究。

The purpose of this study is to save the species ' genetic resources to facilitate the screening and studying of related genes by building the O. sinensis cDNA library .

将上述定位的鸡的20个功能基因的BAC克隆分别与利用胚胎成纤维细胞制备的鸭和鸵鸟的中期染色体杂交。

The above BAC clones of20 chicken genes were hybridized by FISH with duck and ostrich metaphase chromosomes prepared from embryo fibroblasts .

这些生长相关性状的QTL及功能基因的定位为我们从事分子标记辅助育种和功能基因的图位克隆提供了参考区间。

The location of functional genes and QTLs associated with the growth would be very useful for molecular marker-assisted selection and map-based cloning of functional genes .

对虾血细胞T7噬菌体展示文库构建及功能基因淘选

Construction of T_7 Phage Display Library from Chinese Shrimp 's hemocytes and Panning of Functional Genes from the Library

主要研究结果如下：1.本研究从实验室已鉴定的348个EST筛选了与马铃薯晚疫病水平抗性相关的未知功能基因的EST35个。

The main results are as follows : 1 . There were 35 function-unknown genes selected from previously identified 384 ESTs associated with potato horizontal resistance to P. infestans . 2 .

用20个微卫星标记和5个功能基因对整个文库进行PCR筛选，所有这些标记和基因都得到了阳性克隆，克隆数从3到19不等，得到的平均阳性克隆数为11.3。

The library was screened by using 20 microsatellite markers and 5 known functional genes with PCR . Positive BACs for all these markers and genes were identified successfully and the positive number ranges from 3 to 19 with average of 11.3 .

膜蛋白基因Itm2c：神经元分化相关基因Mib等，以及大量未知功能基因和未测序克隆。

Membrane protein gene Itm2c ; neuron differentiation gene Mib and lots of function unknown genes and un-sequenced clones .

双功能基因APE1敲低后人体骨肉瘤细胞基因表达谱变化的研究

The altered gene profile of human osteosarcoma cell after knock-down of double functional gene apurinic / apyrimidinic endonuclease

本实验建立的鸭β-actin基因实时荧光定量PCR法扩增效率高、线性范围广、检测周期短，为β-actin基因作为内参基因进行鸭功能基因与病原基因表达的定量分析奠定了基础。

The real-time PCR assay developed in this study can detect β - actin in broad range with high efficiency and short time . The assay provide the basis for β - actin gene of duck as a reference gene in quantitative analysis of duck mRNA expression .