拷贝数

增生性瘢痕与瘢痕疙瘩DNA拷贝数变化的差异分析

Differences of DNA copy number changes between hyperplastic scar and keloid

喉癌线粒体DNA拷贝数的临床意义

Clinical significance of increase in mitochondrial DNA copy number in laryngeal squamous cell carcinoma

应用引物延伸预扩增提高微量DNA拷贝数

To increase copy of small DNA sample by primer-extension preamplification

乳腺癌线粒体DNA拷贝数改变的研究及意义

Study on copy numbers alteration of mitochondrial DNA in breast cancer tissue

肺鳞癌转移相关基因组DNA拷贝数改变的研究

A Study on DNA Copy Number Alterations Related to Metastasis in Lung Squamous Cell Carcinomas

最后对本研究中用于进行病毒绝对拷贝数定量的实时荧光定量PCR的技术原理和应用前景进行了简要介绍。

The principle of mechanism of Real-Time Quantitative PCR is introduced .

方法用PCR定量法检测血清中HBVdna拷贝数，以及与转氨酶的相关性。

Methods HBV-DNA copies in patients were detected by PCR quantitative method .

病例3和病例5其基因组DNA分别合并有重复和缺失的复杂DNA拷贝数变异。

Case 3 and case 5 : There are the cases of complex genomic duplications and deletions .

起始拷贝数越多，Ct值越小。

The more number the template onset copy has , and less the Ct value will be .

用实时定量PCR检测标本线粒体DNA的拷贝数。

Moreover , To detect the mitochondria DNA copy numbers of sample with real-time PCR .

利用实时荧光定量PCR方法分析转基因水稻外源基因拷贝数

Estimating copy number of transgenes in transformed rice by real time quantitative PCR

结论：线粒体DNA拷贝数改变与乳腺癌发生有关。

Conclusion : It is relative that copy numbers alteration of mitochondria DNA with generation of breast cancer .

从病毒悬液中提取DNA，采用实时荧光定量PCR检测病毒基因拷贝数。

Extracted DNA from virion suspension and quantified viral genomes copies by fluorescence quantitated real-time PCR ;

转基因马铃薯植株中外源基因PCR扩增和拷贝数测定

PCR Amplification and Copy Number Analysis of Foreign Gene in Transgenic Potato Plants Hasi Agula

一种能精确检测人间期细胞核中21号染色体拷贝数的DNA探针

A DNA Probe Suitable for the Detection of Chromosome 21 Copy Number in Human Interphase Nuclei by Fluorescence in Situ Hybridization

实时荧光定量PCR检测毕赤酵母基因组中外源基因拷贝数

Determination of Copy Number of Foreign Gene in Genome of Pichia pastoris by Real-time Fluorescent Quantitative PCR

用荧光定量PCR方法检测转染细胞中外源基因的拷贝数

Detection of the Copy Numbers of Exogenous Gene of Transfected Cells by Fluorescent Quantitative Polymerase Chain Reaction

荧光定量PCR检测细菌染色体上基因拷贝数

The copy number of the gene in chromosome of strain detected by the real time quantitative PCR

Southern杂交分析显示大部分株系呈阳性，拷贝数1-4。

Southern blot analysis indicated that most transgenic lines are positive and gene copy number ranged frome 1 to 4.2 .

荧光定量PCR法检测重组汉逊酵母中HBsAg基因的拷贝数

Estimation of the HBsAg Gene Copy Number of Recombinant Hansenula Polymorpha Yeast by Fluorescence Quantitative PCR

Southern杂交结果显示，盐藻烯醇酶基因已整合到植物基因组中，在转基因烟草中的拷贝数为2～4个。

The Southern hybridization results showed there were 2 ~ 4 copies of Dunaliella salina enolase gene in the transgenic tobacco .

应用实时定量PCR，检测秀丽线虫的线粒体拷贝数（mtDNA）。

The mtDNA copy number of C. elegans was determined with the quantitative PCR .

本研究以细菌染色体之外的遗传因子&质粒为对象，研究La3+对质粒DNA复制拷贝数的影响。

So the genetic factor-plasmid is used as experimental material to research the effect of La (ⅲ) on replication copy number of plasmid DNA .

实时定量PCR方法检测鼻咽癌病人全血EB病毒拷贝数的实验研究

Real-time quantitative PCR assay of Epstein-Barr virus in whole blood cells of nasopharyngeal carcinoma patients

Southern杂交分析表明若处于很强的选择压力下，该质粒的拷贝数会发生有效的扩增。

By means of southern hybridization analysis , the efficient amplification of plasmid copy number was detected under a strong selection pressure .

检测上清PGE2分泌、细胞增殖和细胞PPAR-γmRNA拷贝数。

Cell proliferation , PGE_2 secretion and PPAR - γ mRNA were detected .

对部分PCR阳性株进行Southern杂交检测，证明外源基因已经整合到植株基因组中，而且拷贝数较低，为1-2个拷贝。

Southern blot analysis of part of positive transgenic plants revealed that the exogenous gene has been integrated into the plant genome .

病毒RNA分子拷贝数和靶基因mRNA水平用实时定量RT-PCR确定。

The RNA copies of viral vectors and mRNA levels of target genes were determined by quantitative real-time RT-PCR .

而亲本Ds元件拷贝数对Ds的跳跃频率影响不大。

It was found that Ds copy number had little influence on Ds transposition frequency .

经检验，每个PCR反应孔的理论cDNA模板拷贝数为0.44个，说明扩增反应是特异有效的。

Upon examination , each PCR reaction contains theoretical 0.44 copy number of cDNA template , indicating that amplification was specific and effective .