副溶血性弧菌

多抗的ELISA检测结果显示制备所得多抗对副溶血性弧菌具有较强的特异性反应，同时对单增李斯特菌、金黄色葡萄球菌、铜绿假单胞菌也有较弱的交叉反应。

It was found that the polyclonal antibody can well identify Vibrio parahaemolyticus , while it has the weak response to Listeria monocytogenes , Staphylococcus aureus , Pseudomonas aeruginosa and an unknown strain of Vibrio by the ELISA detection . 4 .

实验室检测一起副溶血性弧菌食物中毒报告

Laboratory testing with Vibrio parahaemolyticus food poisoning cases

应用双重Real-timePcR同步定量检测食品中的副溶血性弧菌及金黄色葡萄球菌

Duplex Real-Time PCR for Quantitative Detection of Vibrio parahaemolyticus and Staphylococcus aureus in Foods

实时荧光PCR检测水产品中副溶血性弧菌

Real-time fluorescence PCR detection of Vibrio parahaemolyticus in aquatic products

副溶血性弧菌流行群特异多重PCR鉴定

A pandemic group-specific multiplex PCR assay application in identification of pandemic vibrio parahaemolyticus

副溶血性弧菌ELISA快速检测试剂盒的研制

Initial development of ELISA kit for rapid detection for Vibrio Parahaemolyticus

用PCR检测副溶血性弧菌耐热溶血素基因

Detection of the Thermostable Direct Hemolysin ( tdh ) Gene of Vibrio parahaemolyticus by

沙门菌、志贺菌、副溶血性弧菌多重PCR检测方法的研究

Multiple PCR Detection Method for Salmonella spp. , Shigella spp. , and Vibrio parahaemolyticus

副溶血性弧菌全基因组DNA芯片和比较基因组学研究伤寒沙门菌基因组DNA芯片基因表达谱分析技术的建立与优化

Comparative and Evolutionary Genomic Analysis of Vibrio Parahaemolyticus Development and optimization of DNA microarray-based Salmonella enterica serovar Typhi gene expression profiling

快速荧光定量PCR检测副溶血性弧菌及其毒力基因方法建立与应用效果评价的研究

Rapid fluorescent PCR detection of Vibrio parahaemolyticus and ways of its virulence genes setting up and evaluation of application effect

结果运用群特异多重PCR法可特异性的扩增出当前流行群副溶血性弧菌的目的基因片段，可大大缩短鉴定时间。

Results The aim gene fragment can be amplified by a multiplex PCR assay , it largely reduce the time of identification .

副溶血性弧菌tdh基因的分子信标PCR技术检测

Detection of thermostable direct hemolysin gene in Vibrio parahaemolyticus by molecular beacon PCR

大肠杆菌O157的致病性：1例大肠杆菌O157∶NM和副溶血性弧菌的重叠感染

A case report of coinfection of Escherichia coli o157 : nm and Vibrio parahaemolyticus

前言：目的建立适于基层单位应用的针对副溶血性弧菌流行群的特异多重PCR方法。

Objective In order to set up rapid identification for the pandemic vibrio parahaemolyticus , develope a pandemic group-specific multiplex PCR assay for base unit .

广东省副溶血性弧菌rDNA指纹图谱特征分析

Analysis of rDNA Fingerprinting patterns of Vibrio parahaemolyticus isolated from food and diarrhea patients

副溶血性弧菌对痢特灵（FR）的耐药率为66.3%，其次是氨苄西林（AMP），为61%。

Drug resistance of V.parahaemolyticus to FR and AMP were 66.3 % , 61 % respectively .

目的探讨克隆副溶血性弧菌（Vp）耐热直接溶血毒素基因的方法。

Objective To clone thermostable direct hemolysin ( TDH ) gene ( tdh gene ) of Vibrio parahaemolyticus .

采用MPN法进行副溶血性弧菌定量检测；

The Vibrio parahaemolyticus was determined qualitatively by MPN method .

副溶血性弧菌ERIC-PCR分型及毒力基因的检测研究

Genotyping of Vibrio parahaemolyticus by ERIC-PCR and Virulence Gene Detection

聚合酶链反应检测粪便中的副溶血性弧菌的TDH基因

Detection of TDH Gene of Vibrio Parahaemolyticus in Diarrheal Stool Using Rapid PCR

方法：用MPN法检测静冈县海水、海泥中副溶血性弧菌数。

Method : Detect the number of V.p in seawater and sea sand by MPN method .

对302株副溶血性弧菌溶血素检测结果表明，耐热溶血素（TDH）阳性213株，占70.5%；

Hemolysin monitoring showed 213 strains of V.parahaemolyticus with positive TDH , accounting for 70.5 % .

为模拟生牡蛎消费引起副溶血性弧菌（VP）疾病的危险性，在福建省开展了定量微生物危险性评估。

A quantitative microbial risk assessment was undertaken to model the risk of Vibrio parahaemolyticus foodborne illness associated with the consumption of raw oysters in Fujian Province , China .

结果267株副溶血性弧菌中27株尿素酶呈阳性，其中14株菌tdh基因和trh基因呈阳性。

Results 27 strains were positive for urea hydrolysis , of which 14 strains were positive for tdh and trh genes .

扫描电镜下观察DMY作用后的副溶血性弧菌，其形态与对照组相比有明显的改变。

The vibrio parahaemolyticus affected by DMY has been significantly changed through scanning electron microscopy compared with the control group .

药敏试验结果显示，沙门菌、副溶血性弧菌、O157∶H7大肠杆菌均有多重耐药株，单核细胞增生性李斯特菌对抗生素产生耐药性的比例较低。

Antibiotic susceptibility tests revealed that Salmonella , Vibrio parahaemolyticus and EHEC O157 ∶ H7 had multiple antibiotic resistant strains , but most Listeria monocytogenes strains showed high antibiotic susceptibility .

副溶血性弧菌（V.parahaemolyticus，Vp）是我国一种重要的食源性病原菌，主要引起急性肠胃炎，少数情况下诱发败血症。

Parahaemolyticus ( Vp ) is one of the most important foodborne pathogens in our country and can cause acute gastroenteritis in most cases or blood poisoning in few of cases .

副溶血性弧菌是细菌性腹泻的主要致病因子.本研究建立一种快速标本处理和聚合酶链反应（PCR）诊断方法，扩增副溶血性弧菌的热稳定直接溶血素（TDH）基因。

Vibrio Parahaemolyticus is the principal pathogenic factor causing acute bacterial diarrhea . A method using the rapid PCR to detect the virulence gene , the thermostable direct hemolysin ( TDH ) of Vibrio para-haemolyticus , in diarrheal stools was set up .

以体积分数为3.13%大蒜液与3.13%食醋组成的复方溶液对副溶血性弧菌作用2min，杀菌率亦为100%。

The solution composed of 3.13 % ( v / v ) garlic solution and 3.13 % vinegar with a 2 min contact time also killed 100 % of V.parahaemolyticus .

方法在反应体系中加入分子信标探针，对13株副溶血性弧菌和其他细菌分别进行tdh基因实时和终点法荧光检测。

Methods The tdh gene of 2 strains of Vibrio parahaemolyticus and 13 strains of other enteric pathogens was respectively amplified in the PCR reaction system containing a molecular beacon probe , and record the fluorescent value in the terminal of PCR and during the real time PCR .