免疫磁珠法

  • 网络macs;minimacs;immunomagnetic beads;immunomagnetic bead separation
免疫磁珠法免疫磁珠法
  1. 免疫磁珠法是高效纯化T细胞的新方法。

    MACS is a new purifying method of high efficiency .

  2. 与其他分选方法相比,免疫磁珠法具有分离效率高,细胞活性好,操作简便等优点。

    MACS technology has many advantages over other cell sorting methods , such as high efficiency , high cell viability and easiness to operate .

  3. 免疫磁珠法在肾移植HLA快速分型中的应用

    Application of Immunomagnetic Beads Technique for Tissue Typing in Renal Transplantation

  4. 应用免疫磁珠法分离脐血CD(34)~+造血细胞

    Immunomagnetic positive selection of cd_ ( 34 ) ~ + progenitor cells from human cord blood

  5. 羟基淀粉沉淀对免疫磁珠法分离脐血CD(34)~+细胞及造血祖细胞增殖功能的影响

    Colony culture and purification of CD_ ( 34 ) ~ + hematopoietic stem / progenitor cells from cord blood by MACS and HES

  6. 免疫磁珠法分选小鼠肝脏Kupffer细胞及其特性观察

    Isolation and purification of mouse liver Kupffer cells by immunomagnetic bead separation

  7. 分离骨髓单个核细胞,用免疫磁珠法富集CD34+细胞、CD4+(Th)细胞。

    Bone marrow mononuclear cells ( BMMNC ) were isolated and CD34 + and CD4 + cells were enriched by magnetic beads respectively .

  8. 〔方法〕采用PCR法、免疫磁珠法和神奈川现象培养基三者结合使用。

    〔 Methods 〕 PCR was used in combination with immunomagnetic separation and Kanagawa phenomenon culture medium to determine the isolation rate of TDH + V.p strains from environment .

  9. 方法:采用美国NIH标准微量淋巴细胞毒技术和免疫磁珠法分离TB淋巴细胞。

    Methods : T , B lymphocytes were isolated by American NIH standard lympho cytotoxic technique and magnetic beads method .

  10. 用Percoll密度梯度及免疫磁珠法分离骨髓巨核细胞;

    Percoll density gradient and immunomagnetic beads methods were used to purify megakaryocytes from bone marrow .

  11. 方法应用免疫磁珠法(MACS)分选C57BL/6鼠CD4+CD25+T细胞。

    Methods CD4 ~ + CD25 ~ + regulatory T cells were purified from C57BL / 6 mouse and using MACS .

  12. 结论免疫磁珠法是分离脐血CD133阳性细胞的较好的方法。

    Conclusions Magnetic cell sorting was a good method for separating CD133 + cell from cord blood .

  13. 方法采用免疫磁珠法测量转染WeelHu基因的NIT细胞培养上清中的胰岛素水平及观察其增殖速度。

    Methods The level of insulin in culture supernatant of NIT cells was measured by immune magnetic bead method and their growth rate was observed .

  14. 结论:本实验通过免疫磁珠法(CD11b负选)成功地从FVB/N小鼠骨髓细胞中分离纯化出骨髓间充质干细胞。

    CONCLUSION : MSCs are isolated and purified successfully from bone marrow cells of FVB / N mice with immunomagnetic beads method ( CD11b negative selection ) in this trial .

  15. 方法:1、应用CD34+干细胞分选试剂盒和免疫磁珠法,经磁式细胞分选器(MACS)分离出人脐血CD34+干细胞;

    Methods : ( 1 ) CD34 + hematopoietic stem cells were isolated from healthy human cord blood using CD34 + progenitor cell isolated kit by a high-gradient magnetic cells sorting system ( MACS ) .

  16. 用Ficoll-Hapaque分离法分离动员的外周血(MPB)单个核细胞,免疫磁珠法分离纯化CD34+细胞,并将其在含胎牛血清的液体培养体系中、各组细胞因子诱导下培养15天。

    Mononuclear cells were isolated from mobilized peripheral blood ( MPB ) by density gradient centrifugation over Ficoll . CD34 + cells were purified by using an immunomagnetic bead separation system .

  17. 方法:选2例重型再障(severaplasticanemia,SAA)患者及1例正常人,用免疫磁珠法分离纯化外周血CD8+T细胞。

    METHODS : CD8 + T cells were isolated from peripheral blood of two patients with severe aplastic anemia ( SAA ) and one normal person by Ficoll-Hypaque gradient centrifugation and then were purified by immunomagnetic microbead separation .

  18. 方法利用免疫磁珠法(MACS)分离纯化脐血CD34+细胞,在无血清培养体系中以1×105/ml每孔接种,经不同细胞因子组合诱导培养。

    Methods CD34 ~ + cells were isolated from umbilical cord blood by using a high-gradient magnetic activated cell sorting ( MACS ) system , and cultivated by 1 × 10 ~ 5 / ml in serum-free stem cell growth medium ( SCGM ) with different combination of HGFs .

  19. 免疫磁珠法富集循环肿瘤细胞研究进展

    New advancements in the enrichment of circulating tumor cells by immunomagnetic beads

  20. 免疫磁珠法分离白血病转基因小鼠骨髓造血干细胞

    Isolation of Bone Marrow Hematopoietic Stem Cells from PML-RAR α Transgenic Mice

  21. 免疫磁珠法阳性检出率高于常规培养法。

    The immunomagnetic separation has a higher positive rate than general cultivation method .

  22. 免疫磁珠法分选出CD34+细胞。

    CD34 + cells were divided by immunomagnetic beads selected system ( MACS ) .

  23. 目的:为了建立免疫磁珠法体外分离提纯骨髓造血干细胞的方法。

    Objective : To isolate and purify murine hematopoietic stem cell using the immunomagnetic microbeads .

  24. 联用密度梯度离心与免疫磁珠法分离纯化人表皮朗格汉斯细胞

    Separation and purification of Langerhans cells from human epidermis by density gradient centrifugation and magnetic cell sorting

  25. 方法免疫磁珠法分选脐血中CD34+细胞,接种到预先照射的基质层上。

    Methods Immunomagnetic selected CD34 + cells were inoculated onto the pre established irradiated human stroma layer .

  26. 免疫磁珠法分选骨髓CD34~+细胞:纯度影响因素的分析

    Immunomagnetic beads method for separating bone marrow CD34 ~ + cells : An analysis of purity influencing factors

  27. 免疫磁珠法分离纯化骨髓间充质干细胞及向成软骨细胞分化

    Isolation , purification and directed differentiation to cartilage cell of bone marrow mesenchymal stem cells by immunomagnetic beads method

  28. 免疫磁珠法分选人骨髓多能成体祖细胞及初步鉴定

    In vitro purification and culture of multipotent adult progenitor cells from human bone marrow by magnetic activated cell sorting

  29. 为寻找更有效的体外扩增脐血CD34+细胞的造血细胞因子组合,采集健康产妇脐带血,用免疫磁珠法分选CD34+细胞。

    The objective was to explore the optimal cytokine combinations for in vitro amplification of the cord blood CD34 + cell .

  30. 免疫磁珠法检测系统性红斑狼疮患者外周血T淋巴细胞亚群及其意义

    Detection and significance of CD4 and CD8 subsets of T lymphocytes in the peripheral blood of systemic lupus erythematosus patients by magnetic activated cell sorting