共转化

双T-DNA共转化获得转基因番木瓜的研究

Generation of Transgenic Carica Papaya Using Two-T-DNA Co-transformation

利用高效CaCl2转化法实现质粒的共转化

Co-transformation of Plasmids by Efficient CaCl_2 Transformation

比较其转化效率，结果基因组DNA转化率大于基因两个片段的共转化率，更大于不加携带者DNA的共转化率。

These phage DNAs are in pairwise combinations and divided into two groups-with or without carrier DNA during the process of cotransfection .

无载体主干序列的bar和cecropinB基因表达框共转化水稻

Cotransformation of Rice by bar and cecropin B Gene Expression Cassettes Lacking Vector Backbone Sequences

Anti-trxs和Bar基因共转化小麦后代植株的遗传及表型分析

Inheritance and phenotype analysis of Anti-trxs gene and Bar gene in transgenic wheat

对共转化的pC-3-1细胞中乙型肝炎病毒DNA存在状态及HBsAg表达稳定性的研究

State of Hepatitis B Virus DNA and Stability of Expression of HBsAg in Transformed pC-3-1 Cells

通过共转化法培育无抗性选择标记的转反义Wx基因水稻的研究

Co-transformation of Rice for Production of Selectable Marker-free Transgenic Plants Containing Antisense Wx Gene

利用不相容质粒共转化大肠杆菌对Cre重组酶体内重组活性的可视检测

A simple and visible assay for cre recombinase activity in Escherichia coli by using two incompatible plasmids

PCR检测PStVCP和Hyg~r均导入花生基因组中，两基因共转化率为50%。

PCR test of To generation plants indicated the PStV CP gene and Hyg ~ r have been inserted into peanut genome .

最终，获得了5株转基因再生植株，经PCR和Southern杂交检测结果表明，只有2株为共转化的结果，诱导了其中1株生根并移栽。

Five regenerated transgenic plantlets were obtained , of which two were proved to be the result of co-transformation using PCR assay and southern blot , and a co-transformed one was induced to root and transplanted . 6 .

为彻底消除选择标记基因的负面影响，目前已经发展了多种去除选择标记基因的植物转化系统，如共转化系统、位点特异性重组系统、转座子系统及MAT载体系统等。

Presently many plant transformation systems have been developed in order to remove selectable marker genes such as co-transformation system , site-specific recombination system , transposable element system and MAT vector system etc.

半夏凝集素在水稻抗虫上的研究及共转化的初步探讨掌叶半夏凝集素的分离纯化及其在体内外对小鼠肉瘤S180细胞的影响

Pest-resistance of Pinellia Ternata Agglutinin and Establishment of Gene Co-transformation System in Rice ; Isolation and Purification of Pinellia Pedatisecta Lectin and Its Effects on Mouse Sarcoma S180 Cells in Vivo and in Vitro

同时，将MIFsiRNA表达质粒分别与MIF表达载体共转化HEK293细胞，用荧光定量PCR检测HEK293细胞中MIFmRNA的表达水平。

The 3 candidate MIF siRNA expression plasmids were also co-transfected with MIF expression plasmid into HEK 293 cells , respectively , and the MIF mRNA expressions were determined by real-time quantitative PCR .

分析了T0代发生共转化的13个T1代株系，共951株植株，分离得到只具有sck基因，无选择标记基因的植株为166株，其比率为17.46%。

The percentage of co-transformation with hpt and sck were 63.93 % . 166 marker free transgenic plants were obtained by PCR from 13 of lines which developed from TO plants .

杨树基因枪多基因共转化研究

Multiple Gene Co - transformation of Populus Via Microprojectile - bombardment

共转化法删除转基因植物筛选标记基因的研究

Studies of Co-transformation for the Elimination of Selective Marker Genes from Transgenic Plants

Δ~6-脂肪酸脱氢酶基因克隆及其共转化表达载体的构建

Cloning of Δ ~ 6-Desaturase Gene and Construction of Its Co-transformation Expression Vector

应用共转化技术在哺乳动物细胞中表达乙型肝炎病毒表面抗原

Expression of HBsAg in Mammalian Cells by Cotransformation

整合载体与选择载体共转化酵母菌的研究

Multicopy integrating plasmid and selective plasmid co-transforming yeast

将以上两重组质粒共转化E。

These two expressing vectors were introduced into E.

煤与甲烷共转化过程中煤焦二氧化碳气化动力学研究

A kinetic study of CO_2 gasification of a Chinese coal char during combined coal gasification and CH_4 reforming

结论：实现了β-甘露聚糖酶基因随共转化工程菌染色体稳定遗传及表达的目的。

Conclusion : Successful expression β - Mannanase gene in wide type yeast and it can stability hereditary .

双菌株法中两种菌液不同浓度比对共转化率的影响

Effect of the Different Concentration of Two Strains on the Rate of Co-transformation in Two Plasmid / Two Strain Method

实现多基因在同一受体植株中的共转化在研究植物多基因控制的代谢途径和获得去选择标记转基因植株中具有巨大的应用前景。

Multi-genes co-transformation is very important for studying on metabolic pathway which were controlling by Multi-genes and generation maker-free transgenic plants .

同时，还探讨了这两个基因在棉花中的共转化实验，希望它们的协同增效能进一步提高棉花的抗病性。

In order to evaluate the cooperation effect of the two genes to cotton disease resistance , co-transformation tests were also done .

共转化的效率为4.3×105，重组效率为1.9×106，筛选的克隆数为4.3×105。

Cotransformation efficiency was 4.3 × 10 5 and recombination efficiency was 1.9 × 10 6 , and 4.3 × 10 5 clones were screened .

结果证明：在共转化植株后代中，通过筛选可获得无抗性标记基因的水稻转基因植株。

The results showed that the co-transformation system of mediated by Agrobacterium is one of efficient method to generate transgenic plants of rice with marker-free .

因此，基因枪介导基因表达框共转化后应用遗传分离策略剔除标记基因，为培育安全转基因植株开创了有效新途径，是本研究的创新点之一。

Therefore , it provide a effective new way to generate safe transgenic plant by removing marker genes through hereditary segregation after co-transformation of separate GECs via particle bombardment .

本文综述了共转化系统、位点特异性重组系统（包括FLP/FRT、Cre/lox、R/RS及Gin/gix系统）和转座子系统（Ac/Ds转座子系统）在培育无选择标记转基因植物中的应用。

The using of co-transformation system , site-specific recombination system ( FLP / FRT ? Cre / lox ? R / RS and Gin / gix systems ) and transposable element system ( Ac / Ds transposable element system ) in breeding marker-free transgenic plants were reviewed in this paper .

一种磷酸钙DNA共沉淀转化哺乳化物细胞较易成功的简易变通方法

An Easy Available Modified Method of Calcium Phosphate Co - precipitation With DNA for Transfection of Mammalian Cells