克隆牛

我国体细胞克隆牛的研究进展

The Study on Somatic Cell Cloned Cattle in China

通过微卫星DNA及线粒体DNA检测，分别对5头克隆牛核内和核外DNA进行了分析。

The nuclear and cytoplasm DNAs of five cloned cattle were detected by microsatellite DNA analysis and mitochondrial DNA analysis , respectively .

2008年，美国食品药品监督管理局（FDA）裁定克隆牛的做法安全。

In 2008 the US Food and Drug Administration ruled that the practice was safe ;

目的：克隆牛白细胞介素18（IL-18）全基因，并对其进行序列分析。

Objective : To clone a cDNA of bovine interleukin-18 and analyze sequence .

转GFP基因体细胞核移植克隆牛胚胎的研究

Study on Transgenic GFP Gene in Bovine Embryos Production by Somatic Cell Nuclear Transfer

结果表明克隆牛的微卫星DNA与核供体牛完全一致，而线粒体DNA则全部来源于卵母细胞。

The results of the present study indicate that the microsatellite DNA of cloned cattle is consistent with the nuclear donor absolutely . However , their mtDNA is originated from corresponding oocytes completely .

中国生物技术公司博雅（BoyaLife）将与韩国研究公司秀岩生命工学研究院（SooamBiotech）组建合资企业，每年将生产100万头克隆牛，以满足公众对优质牛肉的需求。

BoyaLife , a Chinese biotechnology company , is teaming up with a Korean research company , Sooam Biotech , to clone 1m cattle a year to feed public demand for high-quality beef .

体细胞克隆牛0～12月龄生长的测定研究

John Bull Growth Measurement of Holstein Calves Cloned from Somatic Cells

体细胞核移植克隆牛的遗传物质鉴定

Identification of Genetic Materials in Cloned Cattle by Somatic Cell Nuclear Transfer

组蛋白乙酰化在体细胞克隆牛肺脏中的研究

Histone Acetylation Study in Lung of Somatic Cloned Cattle

5头经玻璃化冷冻保存后移植的成年体细胞克隆牛降生

Five calves born after transfer of vitrified embryos derived from adult somatic cell cloning

中国本土克隆牛的诞生

Great moment of cloned cattle 's birth

胚胎无血清体外培养体系的建立与克隆牛微卫星检测

Establishment of a Serum-Free in Vitro Culture System for Embryos and Microsatellite Assay of Cloned Cattle

对克隆牛中组蛋白乙酰化水平和基因在克隆牛中的表达水平对比分析，我们发现两者并没有某种一致的联系。

However , it is difficult to relate the histone acetylation level with the gene expression level .

科研人员希望完善克隆牛技术，因为昂贵的高质牛肉市场庞大。

The researchers want to perfect cloning in cows because of the large market for expensive high-quality beef .

克隆牛报道引发的思索

Thinking of clone caws

另一方面，美国已经连续好几年成功克隆牛。

In the US , on the other hand , cattle have been cloned successfully for several years .

一只克隆牛出生后6周内发育正常，而后莫名其妙地停止产生红血球而死亡。

One cloned calf developed normally for six weeks and then mysteriously stopped producing red blood cells and died .

目的从牛原始生殖细胞中分离克隆牛胚胎干细胞。

Objective : To isolate and clone bovine embryonic stem cells from bovine fetus ' Primordial germ cells ( PGCs ) .

体细胞克隆牛超排胚胎玻璃化冷冻及移植试验

The Experiment on the Cloned Cattle Derived from Somatic Cells and the Transfer of the Vitrified-Thawed Embryos of the Cloning Cattle

食品与药品管理局表示成年克隆牛，猪，羊的肉奶产品可以安全食用。

The Food and Drug Administration says meat and milk from clones of adult cattle , pigs and goats are safe to eat .

他们擅长为医疗领域克隆牛和猪、保存好幼崽，尤其擅长培养基因工程学的动物以用作疾病研究范本。

They specialise in cloning cattle and pigs for medical research and breed preservation as well , particularly developing genetically-engineered animals for use as disease models .

他计划“一期”每年生产10万个克隆牛胚胎，未来将每年生产100万个。

Starting with 100000 cloned cattle embryos a year in " phase one , " Mr Xu envisages 1m annually at some point in the future .

美国政府发布了一份建议草案，声称食用克隆牛、猪和山羊似乎与以传统方式饲养的动物同样安全。

The US government has released a draft proposal declaring that food from cloned cattle , pigs and goats is " likely to be as safe as " food from their non-cloned counterparts .

本研究在克隆表达牛肌钙蛋白I的基础上，研制了针对肌钙蛋白I的牛羊特异性的单抗，并进一步建立了肉骨粉检测的夹心ELISA方法。

In this study , we developed a sandwich ELISA for ruminant MBM , basing on monoclonal antibodies to skeletal troponin I .

人gdnf基因的克隆及牛β-酪蛋白基因座定位整合载体的构建

Construction of Targeting Vector for the Human GDNF cDNA Knock-in at the Cattle Beta-casein Locus

通过重组PCR方法，将克隆的牛BLG上游调控成分与猪瘟病毒E2基因连接起来，并克隆到pMD18-T载体的T位点，形成pBE质粒。

BLG regulation sequence and CSFV E2 gene are linked by method of recombinant PCR , and then they are inserted into pMD 18-T vector . The positive recombinant plasmid is marked as pBE .

从那时起，中国科学家克隆了牛和猪，但这种活动很大程度只限于实验目的。

Since then , Chinese scientists have cloned cows and pigs , but they have largely limited such activities to experimental purposes .

观察了波尔山羊体内受精、波尔山羊耳皮肤成纤维细胞克隆、牛-山羊异种克隆三者的早期胚胎超微结构，从亚细胞水平对两种克隆胚胎进行了评定。

The Boer goat ear skin fibroblast nucleo-transferred embryos and bovine-goat ( Boer ) interspecies clone embryos have been evaluated by studying the ultrastructure of Boer goat embryos in vivo .

结论：成功地从牛外周血中克隆到了牛IL-18的基因，其全长为598bp。

Conclusion : BoIL-18 from the bovine peripheral blood lymphocyte has been cloned successfully and its full length is 598 bp .