伤寒杆菌

伤寒杆菌H抗原检测、肥达反应与细菌培养对伤寒诊断的对照观察

Contrast observation on typhoid bacilli H antigen detection , widal reaction and bacterial culture for diagnosing typhoid

伤寒杆菌H、O抗体的ELISA检测及临床应用

An ELISA Assay to Detect Bacilus Typhi H 、 O Antibodies and Its Clinical Use

甲型肝炎病毒编码区cDNA在减毒伤寒杆菌中的高效表达

The expression of hepatitis A virus coding region cDNA in attenuated Salmonella typhimurium

实验室检测结果：血液及肛拭标本伤寒杆菌培养阳性。血清肥达氏试验特异性O抗体阳性，H抗体阳性。

The laboratory result was : the typhoid bacterium were isolated from case samples O and H antibody were positive .

伤寒杆菌质粒pR（st98）的DNA限制性内切酶分析和药物的体外消除研究

The Restriction Endonuclease Analysis and Elimination of pR_ ( st98 ) in Salmonella typhi

结论伤寒杆菌H抗原酶联免疫检测快速、特异、敏感为实验室检测的首选方法。

Conclusions : Enzyme-linked immunoassay for detection of typhoid bacilli H antigen was the first selective method for laboratory examination of typhoid because of its fast , specificity and sensitivity .

方法该文采用细菌培养、肥达反应、伤寒杆菌H抗原酶联免疫检测检查52例伤寒患者，20例正常人，进行对照观察。

Methods : We examined and observed 52 patients with typhoid and 20 normal controls by bacterial culture , Widal reaction , and enzyme-linked immunoassay for detection of typhoid bacilli H antigen .

伤寒杆菌耐药质粒pR（ST98）介导血清杀菌作用抗性的研究

Study on the Serum Resistance Mediated by An Antibiotic Resistance Plasmid from Salmonella Typhi

结论：成功构建用8核苷酸随机引物进行逆转录同时测定伤寒杆菌鞭毛抗原z66和d/j基因表达的荧光实时定量RT-PCR方法。

Conclusion : The real time RT-PCR method using 8-mer random primer was much better for investigating mRNA levels of flagellin genes of S. enterica serovar Typhi .

伤寒杆菌耐药质粒pR（ST98）与细菌在巨噬细胞内存活关系的研究

Studies on the Ability of S.typhi Strains Survival within Macrophages Mediated by Drug Resistant Plasmid

伤寒杆菌稳定L型粗糙型返祖菌的蛋白图谱和抗原研究

Study on protein patterns and antigens of rough revertants from stable

小鼠鼻粘膜接种伤寒杆菌Fe-SOD的抗体应答

Antibody response induced by intranasal immunization with Salmonella typhi Fe-SOD in mice

结果细菌培养、肥达反应、伤寒杆菌H抗原的酶免疫检测对伤寒患者的诊断的阳性符合率分别为34.6%，59.6%和86%。

Results : The positive coincident rate of bacterial culture , Widal reaction , and enzyme-lin ked immunoassay for detection of typhoid bacilli H antigen for diagnosing typhoid were 34.6 % , 59.6 % , 86 % , respectively .

目的探讨伤寒杆菌IVB型菌毛激活PKC信号通路诱导THP-1细胞IL-6表达。

Objective To investigate the IL-6 expression in THP-1 cells induced by Type IVB pili of Salmonella Typhi through activation of PKC .

目的研究伤寒杆菌DNA旋转酶A亚单位基因（gyrA）变异与其耐喹诺酮类的关系。

Objective To study the relationship between the gene mutations of DNA gyrase subunit A ( gyrA ) and quinolone resistance in Salmonella typhi .

目的：观察小鼠鼻腔接种伤寒杆菌的Fe-SOD后血液和粘膜系统的抗体应答。

Objective : To observe the antibody response induced by intranasal administration of S. typhi Fe-SOD in mice .

结论IVB型菌毛在伤寒杆菌诱导单核/巨噬细胞产生IL-6过程中是一种重要的刺激因素；PKC参与了该过程的细胞内信号转导。

Conclusion The type IVB pili of Salmonella Typhi play an important role in secretion of proinflammatory cytokine IL-6 , and PKC-dependent pathway participates in that process .

目的探讨伤寒杆菌抗原酶免法（ELISA）、脂多糖-被动血凝试验（LPS-PHA）在伤寒早期诊断中的应用价值。

Objective To explore the value of immunoassay of typhoid antigen ( ELISA ) and lipopolysaccharide-passive hemagglutination assay ( LPS-PHA ) on the early diagnosis of typhoid .

伤寒杆菌抗原酶免法、LPS-PHA在伤寒早期诊断中的应用

Apply of Immunoassay of Typhoid Bacillus Antigen and LPS-PHA on the Early Diagnosis of Typhoid

进一步的免疫学指标显示以伤寒杆菌菌苗作为抗肿瘤疫苗可增强荷瘤小鼠的NK细胞和MΦ细胞的杀伤活性。

The result indicated that the antitumor function of ST vaccine was mediated by the host . The immunological parameters showed that ST vaccine can improve the function of NK and M Φ cells of mice bearing tumors .

一.伤寒杆菌质粒pR（ST98）毒力基因Spv的研究二.乙型肝炎病毒HBx蛋白重组腺病毒载体的构建

ⅰ . Study on the Virulence Gene Spv on the Multidrug Resistant Salmonella Typhi Plasmid pR_ ( ST98 )ⅱ . Construction of the Recombinant Adenovirus Vector of HBx Protein of HBV

58株伤寒杆菌的噬菌体分型结果与MIC测定结果对照，未发现噬菌体分型与药物敏感程度两者的关联性，氟喹诺酮类药物对各噬菌体型均有较高杀菌活性。

By comparing the results of phage-typed of 58 isolates with the results of MICs , no correlation between phage type and susceptibility of S , Typhi to antibacterial agents was found , and fluroquinolones had good bactericidal activity .

方法：小鼠分别用鼠伤寒杆菌核糖体制剂（STRP）加日本血吸虫成虫抗原（SWA）和日本血吸虫成虫抗原免疫后，其体液免疫水平用ELISA检测，保护性免疫力用减虫率表示。

METHOD : Mice were immunized with STRP + SWA and SWA , respectively . Humoral immunity levels of mice were detected by ELISA and protective immunity of mice were indicated by worm reduction rate .

结论伤寒杆菌抗原酶免法（ELISA）、LPS-PHA在伤寒早期诊断中具有较高的应用价值，但二者都有一定的假阳性和假阴性，联合应用对伤寒的早期辅助诊断更有价值。

Conclusions ELISA and LPS-PHA have the good value on early diagnosis of typhoid , but both of them have false positive and false negative . Associated application of ELISA and LPS-PHA for early diagnosis of typhoid has a higher applied value .

结果伤寒杆菌抗原酶免法（ELISA）、LPS-PHA在第7d的检出率分别为85.4%和80.5%，显著高于血培养和肥达氏反应（P<0.05）。

Results The positive rate of immunoassay of typhoid ( ELISA ) and LPS-PHA in typhoid individuals were 85.4 % , 80.5 % on 7th day , both of them were much higher than those of blood culture and Widal test ( P < 0.05 ) .

伤寒杆菌菌苗抗肿瘤的实验研究

Experimental study on antitumor immunity with the vaccine of salmonella typhi

伤寒杆菌活菌计数影响因素探讨

A Study on Effective Factors for Counting Live Bacteria of Salmonella Typhi

酶免法检测从业人员伤寒杆菌抗原及临床意义

Detection of Salmonella typhi antigen in workers with ELISA and its significance

鼠伤寒杆菌外膜蛋白免疫原性初步研究

A primary study on immunogenicity of outer membrance proteine of Salmonella typhimurium

鼠伤寒杆菌主要外膜蛋白作为保护性抗原的研究

Study on Major Outer Membrane Proteins of Salmonella Typhimurium as a Protective Antigen