一步纯化

经一步纯化，商品药用HSA中的许多杂蛋白可被除去，经毛细管电泳分析，纯度与Sigma公司的电泳纯HSA相当，回收率可达85%以上。

A lot of impurities in the commercial HSA had been removed by a single step purification with a recovery of more than 85 % of the protein bound on membrane cartridge . The purified HSA was of comparable purity of that from Sigma company analyzed by capillary electrophoresis .

5′AMPQT4亲和层析一步纯化乳酸脱氢酶

One-step purification of lactate dehydrogenase by 5 ′ - amp-qt_4 affinity chromatography

本文报导了用水平等电聚焦电泳技术由粗酶液一步纯化制得纯青霉素G酰化酶。

Penicillin G acylase enzyme of E. coli was purified in one step by horizontal preparative electrofocusing .

表达产物经金属螯合层析一步纯化，并用westernblot鉴定。

Purify the expressed product by one-step immobilized metal ion affinity chromatography ( IMAC ) and identify by Western blot .

应用此亲和柱可一步纯化蛇毒中的NGF，大大简化了NGF纯化工艺。

This column simplifies the technology of NGF purification .

经旋转等电聚焦电泳，一步纯化31.75倍，回收率为56.25%，比活性达1.02×10~7U／mg蛋白。

75 times with a recovery rate of 56 . 25 % and the specific activity of 1 . 02 X 107U / ml protein .

FPLC一步纯化胰岛素单克隆抗体

Single-step purifying of McAb against insulin with FPLC

在选定的色谱条件下，BLC粗酶液经IDA型Cu2＋－螯合膜色谱柱一步纯化，比活性平均提高4.7倍，回收率为67.7%。

The results showed that under the chromatographic conditions chosen , 67.7 % recovery of BLC attained with an overall 4.7 fold increase in specific activity in a single step .

借助表达蛋白带有6×His标签的特性，采用固相化Ni-NTA亲和层析柱对重组相思豆毒素A链（rABRaA）蛋白进行一步纯化，洗脱峰中目的蛋白的纯度高达98%左右。

The recombinant protein ( rABRaA ) was one-step purified by Ni-NTA metal-affinity chromatography , and its purity is up to 98 % .

以简便快速的一步纯化法从猪垂体浸出液中提取出高纯度pFSH。

A highly purified pFSH was isolated from a porcine pituitary extract with the one & step method using the col - umn . The method is simple and rapid .

葡萄糖苷酶在毕赤酵母中的重组表达及一步纯化

Expression and One-step Purification of Recombinant Glucosidase in Pichia pastoris

用谷胱甘肽-sepharose4B亲和层析柱一步纯化法可简单、快速地得到高纯度的蛋白。

Using Glutathione-sepharose 4B affinity column can simply and rapidly purify the proteins .

离子交换柱层析一步纯化法。

The second method was Ion Exchange Pole Chromatography .

用琼脂糖亲和层析一步纯化蓖麻毒蛋白兔肝谷胱甘肽过氧化物酶的亲和层析纯化

One-step Purification of Ricin by Sepharose 4B Affinity Chromatography Purification of Glutathione Peroxidase from Rabbit Liver by Using Affinity Chromatography

重组人TyrRS可以利用Ni2+柱亲和层析的方法快速方便地被一步纯化。

The recombinant protein was conveniently purified in a single step by metal ( Ni2 + ) chelate affinity chromatography .

最后经过肝素亲和层析一步纯化法，得到高纯度的重组人骨形成蛋白&2二聚体和单体。

Finally , a one-step purification procedure based on the heparin affinity chromatography was implemented to isolate the rhBMP-2 dimers and monomers .

一步亲和纯化制备基因工程人源抗HBs单克隆抗体Fab片段

Affinity purification of engineered human monoclonal antibody HBs Fab fragments

本文报导用改变含盐乙醇浓度从粗品中一步分离纯化HCG的方法。

This paper presents a method of separation and purification about HCG with change the concentration of salt-ethanol from the primitive production .

该工艺实现了一步色谱纯化DNA疫苗的目的，具有简单、快速的优点。最后，为检验纯化后的疫苗免疫效果，分别对小鼠和牛进行免疫。

This technique achieved one-step chromatographic purification of DNA vaccine , with the advantages of simplicity and rapidity . Thirdly , to examine the immune effect of purified vaccine , immune effect evaluation was operated on mice and bovines , respectively .

从300mg木蝴蝶粗提物中一步分离纯化得到25·5mg黄芩素和36·6mg白杨黄素。经高效液相色谱分析，纯度分别为99·2%和100%。

From 300 mg of the crude extracts , 25.5 mg of baicalein and 36.6 mg of chrysin were obtained with a purity of 99.2 % and 100 % , respectively , as determined by high performance liquid chromatography .

鸡卵黄免疫球蛋白的嗜硫性色谱一步分离纯化

One-step thiophilic adsorption chromatography purification of chicken immunoglobulin from egg yolk

根据各项指标综合考虑，选择60%和80%组分进行下一步分离纯化研究。第二步用两种凝胶过滤层析介质对硫酸铵组分进行分离。

The protein of the groups 60 % and 80 % were choused for next purification . Secondly , the ammonium sulfate precipitant was purified by gel filtration chromatography .

结果SEB获得高效表达，并一步将其纯化至均质。

Results The recombinant toxin was expressed efficiently in E.coli and SEB of high purity was obtained .

一步柱层析纯化B-藻红蛋白及其毛细管电泳结合激光诱导检测

One-step Chromatography for B-phycoerythrin Purification and Detection by Capillary Electrophoresis Assay with Laser-induced Fluorescence

抗TNF-α单链抗体的构建、表达及一步柱上纯化和复性

Generation , expression and one-step on column purification and refolding of anti-TNF - α single chain antibody

应用一步亲和层析法纯化转基因山羊乳腺定位表达的组织型纤维蛋白溶酶原激活剂

One step Purification of Tissue type Plasminogen Activator Derived from Expression by Milk Gland of Transgenic Goat Using Affinity Chromatography

所表达的核心蛋白融合有谷优甘肽转移酶，用Sepharose4B&GSH凝胶一步亲和层析纯化即可获得高纯度的核心蛋白、该蛋白具有较好的抗原性和特异性。

Coli . The expressed HCV core protein ( fused with glutathione transferase ) was further purified by affinity chromatography using Sepharose 4B-GSH , and the final products showed good antigenicity and specificity .

表达产物经Ni柱一步分离后得到纯化。

The product was purified one step by Ni NTA column .

并研究了rhG-CSF的分离纯化的条件，一步疏水色谱分离纯化，使rhG-CSF的纯度从53.0%增加到97.8%，质量回收率达到24.0%。

Furthermore , the conditions for the purification of rhG-CSF were also investigated . With only one step of high performance hydrophobic interaction chromatography ( HPHIC ), the purity of rhG-CSF was from 53 % to 97.8 % and the mass recovery was 24.0 % .

高温退火一步非破坏性纯化碳纳米管

One-step Process for Non-Destructive Purification of Carbon Nanotubes by High-temperature Annealing