工作浓度

HRP-兔抗鸡IgG的工作浓度为1∶15000，待检血清和酶标二抗的反应时间均为37℃1h；

The working concentration of HRP-labeled rabit anti-chicken Ig is 1:15000 ;

方法采用MTT法确定阿霉素的工作浓度，以该浓度进行化疗或与热疗的联合，选择温度40℃及42℃，体外作用于K562。

Methods The working concentration of adriamycin against K562 was determined by MTT assay .

通过优化实验条件，确定了单抗60bca包被浓度，兔抗人CD14多抗和HRP-羊抗兔IgG的工作浓度；

The concentration of the capture mAb , polyclonal antibody and HRP-labeled goat-anti-rabbit IgG were determined .

试验结果表明，包被抗原1：80（0.0704mg／ml）稀释，待检血清1：40稀释，酶标二抗1：200稀释，是本方法最佳的工作浓度。

The results indicated that diluting the antigen to 0.0704mg/ml and the serum to 1:40 and the marked IgG to 1:200 were the best reaction condition .

结果确定最适抗原包被量为0.034μg，酶标抗体工作浓度和血清稀释度分别为1∶4800和1∶80；

The optimal coating amount of antigen was 0.034 μ g. and the dilutions of enzyme labelled antibody and serum were 1 ∶ 4 800 and 1 ∶ 80 , respectively .

IgM和IgG最适包被浓度为1μg／ml，HRP－羊抗鼠IgG最适工作浓度为1：2000。

The optimum concentrations were 1 μ g / ml IgM and IgG for coating and 1 : 2000 dilution of HRP-goat anti-mouse IgG for working .

采用MTT法确定阿霉素的工作浓度，并以该浓度进行化疗或与热疗的联合。

The working concentrations of adriamycin against K562 , K562 / ADM , Raji and ARH-77 cell lines were determined by MTT assay . 3 .

结论MB工作浓度和光源光强度、光照时间和距离是影响RNA包膜病毒光诱导灭活效果的4个关键因素，前三者与病毒灭活效果正相关，后者则负相关。

CONCLUSIONS Concentration of MB and illuminating intensity , time and distance of lighting system is the four key factors that mainly affect the photo-induced inactivation of RNA envelope viruses .

建立的PS-ELISA方法，在抗原、血清、酶结合物工作浓度选择时，与常规ELISA方法相比，两法OD值差异不大；

Compared with routine ELISA , the concentrations of antigen , serum and enzyme conjugate were similar between the two methods .

结论通过准确确定3-AT的工作浓度，有效地完成了酵母单杂交的大规模筛选人食管癌细胞cDNA文库的实验。

Conclusion The cDNA library of yeast one hybrid system had been screened successfully in large scale by confirming the matched concentration of 3-AT .

用TMEV和痘苗病毒感染BHK-21细胞，制备病毒特异抗原，确定包被抗原和标准阳性血清的最佳工作浓度；并进行敏感性、特异性、重复性和稳定性实验。

BHK-21 cell line was infected by TMEV and Vaccinia virus to prepare specific virus antigen .

采用改良过碘酸钠法将提纯的兔抗IBDVIgG和兔抗鸡IgG进行辣根过氧化物酶（HRP）标记，效果比较理想，二者工作浓度分别为1：1000，1：1200。

Prepared anti-IBDV rabbit IgG and anti-chicken rabbit IgG were conjugated with horseradish peroxidase ( HRP ) by the reformative sodium periodate method and achieved preferable purpose . Their work concentrations were 1:1000 and 1:12000 respectively .

结果表明，噬菌体工作浓度为1×108PFU/ml、37℃感染2h为最佳检测条件。

It was demonstrated that the optimal working concentration of mycobacteriophage was 1 × 10 ~ 8 PFU / ml and the optimal condition for detection was the infection at 37 ℃ for 2 hours .

农杆菌菌液最佳工作浓度为OD600≈0.6；侵染时间为10min；共培养时间为3d。

The best concentration of bacilli is OD600 ≈ 0.6 ; the best infection time is 10 minutes ; and the co-culture time is 3 days . 3 .

结果实验理想的包被抗体浓度为1μg/ml，血清稀释倍数1∶100，HRP标抗体的最佳工作浓度1∶400。

Results Experiment showed that the optimal concentration of the coating antibody , diluted ratio of serum and McAb with HRP were 1 μ g / ml , 1 ∶ 100 and 1 ∶ 400 , respectively .

本文在实验室条件下从样品稀释度、抗体孵育时间、抗体工作浓度等几个方面对斑点免疫方法（DIBA）进行实验条件优化。

We optimized the experimental condition of DIBA in laboratory from different dilution of samples , different concentration of antibody and different reaction time .

采用棋盘滴定法确定抗原和抗血清的最适工作浓度分别为106CFU·mL-1和1∶2000；

The best working concentration of antigen and antiserum were 106 CFU · mL - 1 and 1 ∶ 2000 separately , determined by using checkerboard titration .

确定了包被浓度和酶标抗体的工作浓度：HRP-3-47-26为1∶100；

The concentration of coating of antigen LPS and HRP-3-47-26 was determined : HRP-3-47-26 , 1 ∶ 100 ;

间接-ELISA中HRP标记的兔抗鹅IgG的工作浓度是1∶200，检测血清的最适稀释度是1∶400，阳性判定标准为OD492≥0.20，且P/N≥2.0。

And working concentration of rabbit anti-goose IgG-HRP antibody was 1 ∶ 200 , the suitable concentration of detected sera was 1 ∶ 400.The standards to estimate positive were OD_ ( 492 )≥ 0.2 , P / N ≥ 2.0 for the Indirect-ELISA method .

通过优化抗原包被浓度、封闭液和金标SPA工作浓度等建立了能够区分口蹄疫自然感染动物和免疫动物的斑点免疫金渗滤法和胶体金免疫层析试纸法。

The research established DIGFA and GICA witch could differentiate the natural infected animals from vaccinated animals by the way of optimizing the concentration of antigen coated on the NC film , the confining liquid and the concentration of colloidal gold labelled with SPA .

方法：收集25例12～28周龄胎儿睾丸标本，免疫组织化学ABC法染色，以正常羊血清代替一抗为阴性对照，TGF-β1受体抗血清的工作浓度为1：100，光镜观察。

Methods : Using immunohistochemical the expression of TGF - β 1 R in 25 cases testis of human fetuses ( estimated fetal ages of 12 weeks to 28 weeks ) were observed . Normal goat serum was used to replace TGF - β 1 R in negative control group .

研究了用A蛋白酶联免疫吸附检测法（PAS-ELISA）检测葡萄卷叶病毒的程序，提出了A蛋白、酶标A蛋白、抗体的适宜工作浓度及样品粗提液稀释倍数。

The suitable concentration of protein-A , enzyme-labelled protein-A and antibody , and the dilution multiple of sample crude extract were ascertained in detecting grapevine leaf-roll virus with young leaves and old leaves and young stems and old stems by PAS-ELISA .

血清工作浓度为1∶200；

Working serum concentration was1 ∶ 200 ;

并确立了测定参数：抗血清与包被抗原的最佳工作浓度分别为1：10000和125ng/mL卵黄蛋白；

The optimum concentration of antiserum and antigen coating were 1:10000 and 125 ng / mL Vitellin , respectively .

根据棋盘试验，确定副溶血弧菌免疫血清最佳工作浓度为1∶200，酶标抗体最佳工作浓度为1∶100，以出现明显清晰斑点者判定为阳性。

The working concentration of serum samples was1 ∶ 200 dilution and that of the enzyme-labeled goat anti-rabbit IgG was1 ∶ 100 dilution by chessboard assay .

方阵滴定确定了抗血清最佳稀释度（1∶2500），包被抗原的最适工作浓度0.45μg/mL，并建立了标准工作曲线。

The most appropriate titration of coating antigen was 0.45 μ g / mL and optimal dilution was ( 1 / 2 ? 500 ) correspondingly .

纯化的8个抗体应用镀金芯片法进行配对检测；通过方阵滴定法确定酶标抗体和包被抗体的最佳工作浓度。

Then we pair bond detected the eight strains of antibodies by gliding chip method , determined the optimal working concentration of coating antibodies and enzyme labelled antibody by square matrix titrimetry .

以包被原Ⅱ进行固相包被，经方阵试验确定包被原Ⅱ最佳浓度为6μg/mL，抗CL&BSA抗体（抗体Ⅱ）的最佳工作浓度为3μg/mL。

By phalanx test , the optimum concentration of CL-OVA and anti-CL-BSA antibody ( antibody II ) were selected to be 6 μ g / mL and 3 μ g / mLrespectively .

因此，我们推荐使用该消毒剂的工作浓度为100%杀灭病毒的稀释度，这将为其临床应用提供依据，对环境消毒和防止流感爆发具有参考价值。

So , we recommend that diluted degree of 100 % killing virus was a likely using concentration . It provide basis for disinfection in prevention of influenza virus and the clinic .

抗原、被检血清和酶标结合物的最佳工作浓度分别为10ug/ml、1∶200和1∶3200。

The optimum concentration of coating antigen was 10 ug / ml , and the sera dilution 1:200 and the conjugate dilution 1:3200 . The results of the new IBV ELISA kit showed that this assay was useful for diagnosing IBV infection .