间接免疫荧光试验

间接免疫荧光试验结果显示，5株单抗和感染了CDV的Vero细胞均能发生反应。

The IFA test showed that all the 5 mABs can react with Vero cells infected by CDV .

2005年发热病人血检阳性率为5.82%，而间接免疫荧光试验检测学生血清抗体阳性率15.31%，媒介按蚊监测捕获中华按蚊154只，未发现嗜人按蚊。

Positive rate was 5.82 % in blood examination of fever patients and 15.31 % in IFA of students , respectively . 154 Anopheles sinensis were captured and no An .

间接免疫荧光试验检测登革IgG的应用

Application of Indirect Immunofluorescent Assay for Detecting IgG of Dengue Virus

间接免疫荧光试验在人群埃可30型肠道病毒IgG抗体检测中的应用

Preliminary application of indirect immunofluorescence assay for detecting IgG antibodies to Echovirus 30

间接免疫荧光试验显示，HA蛋白表达于被感染细胞的胞浆中。

Indirect immunofluorescence assay ( IFA ) demonstrated that HAwas expressed by rPRV-HA in cytoplasm .

RT-PCR和间接免疫荧光试验结果显示体外培养的人巩膜成纤维细胞表达M1受体。

Results The cultured fibroblasts demonstrated mRNA expression of M1 receptor in RT-PCR .

1316只蚊分为116组，经C6／36白纹伊蚊细胞培养和单克隆抗体间接免疫荧光试验检测，全部未分离出DF病毒。

1316 mosquitoes divided into 116 groups were tested and no dengue virus was isolated .

间接免疫荧光试验证明：该组单抗测得不同人群外周血淋巴细胞阳性数与用抗B细胞单抗和直接免疫荧光法测得的结果接近。

An approximate positive rate of peripheral blood lymphocyte ( PBL ) was achieved when these McAbs and the McAb against B lymphocytes were tested by indirect immunofluorescent assay ( IFA ) .

用FITC标记的羊抗兔IgG进行间接免疫荧光试验，可检测到特异性黄绿色荧光。

Specific yellow-green fluorescence was visual in indirect immunofluorescence assay with FITC-labeled goat anti-rabbit IgG .

目的：评价间接免疫荧光试验（IFA）在生殖器疱疹（GH）诊断中的应用价值。

AIM : To evaluate the application of indirect immunofluorescence assay ( IFA ) in the diagnosis of genital herpes .

上述杂交瘤细胞涂片后与日本血吸虫感染兔血清进行间接免疫荧光试验（IFT），以探讨凝集机制。

Immunofluorescent test was carried out with the hybridoma cells and schistosome-infected sera .

间接免疫荧光试验（IFA）显示F12D可识别培养的FCC1/HN。

Moreover , the antibody also recognized the cultured parasites of P.falciparum by indirect immunofluorescent antibody test ( IFA ) .

间接免疫荧光试验结果表明重组NP多克隆抗体能够识别牛副流感病毒3型在感染细胞中表达的NP蛋白，而且在稀释度为1：2560时仍具有识别能力。

Indirect immunofluorescence test showed that the antibody could identify the NP protein expressed by bovine parainfluenza virus type 3 in infected cells , even in the dilution of 1:2560 .

经BUdR加压筛选3次后，重组病毒分别用PCR、间接免疫荧光试验和westernblot进行鉴定，并进行小鼠免疫研究。

The recombinant virus was identified by PCR , indirect immunofluorescent assay ( IFA ) and Western blot after three times of pressure selection with BUdR , and the mice immunization experiment was performed .

自身抗体检测采用间接免疫荧光试验（IIFT）。

Auto-antibodies were detected by indirect immunofluorescence test ( IIFT ) .

目的：建立检测血清中人疱疹病毒6型（HHV6）IgG的间接免疫荧光试验（IFA）。

AIM : To establish an indirect immunofluorescence assay ( IFA ) for detection of serum IgG antibody against human herpesvirus 6 ( HHV 6 ) .

中；将重组质粒转染到宿主细胞COS7细胞中，通过间接免疫荧光试验（IFA），证明该基因在宿主细胞中得到表达。

Then the recombinants were transfected into COS-7 , it was proved that the env gene were expressed in the host by IFA .

[方法]采用杂交瘤技术，获得15株特异性单克隆抗体，随后用酶联免疫吸附试验（ELISA）、免疫印迹法（WESTERNBLOTTING）和间接免疫荧光试验（IFA）对部分免疫显性抗原进行分析。

[ Methods ] 15 monoclonal antibodies ( McAbs ) against T s muscle larva ( ML ) soluble antigens were obtained by using hybridoma technique . The reactivity of monoclonal and polyclonal antibodies were tested by ELISA , Western blotting and indirect immunofluorescence assay ( IFA ) .

质粒提纯后，在脂质体作用下转染BHK-21细胞，通过直接免疫荧光试验和间接免疫荧光试验分别检测P32和CD58在BHK-21中的表达。

After plasmid purification , the acquired recombinant plasmids were transfected into BHK-21 cell with liposome mediated method . the expression of P32 and CD58 were tested by direct immunofluorescence assay ( IFA ) or indirect immunofluorescence assay .

结果：毕赤酵母GS115培养上清液中有目的蛋白的表达，分子量约为44KD，间接免疫荧光试验显示在大鼠肋间肌细胞间有荧光出现。

Results : The target protein was secreted in the supernatant of expression culture medium . Its molecular weight was about 44 KD . There was fluorescence between the mouse intercostals muscle cells .

应用间接免疫荧光试验检测我国正常人和白血病病人血清中嗜T淋巴细胞Ⅲ型病毒抗体

Detection of htlv - ⅲ antibody by immunofluorescence test in China

测鸭出血症病毒间接免疫荧光试验的建立

Development of Indirect Immunofluorescence Assay for Determination of Duck Hemorrhagic Disease Virus

单克隆抗体间接免疫荧光试验检测生殖器单纯疱疹病毒感染

Indirect immunofluorescence assay with monoclonal antibodies in detecting genital herpes simplex virus infection

方法：间接免疫荧光试验等。

Method indirect immune fluorescent testing . 3 ANA testing : With indirect immunofluorescence .

血清间接免疫荧光试验，抗体滴度1∶512。经用大剂量青霉素静脉注射症状消失。

The antibody titre in the serum rose to1 ∶ 512.He was cured with large doses of penicillin .

间接免疫荧光试验鉴定获得的细胞纯度分别是82.7%，90.4%和90.8%；

^ subpopulations was 82 . 7 % , 90 . 4 % and 90 . S % respectively , which was estimated by an indirect immunofluorescence technique .

最后一次免疫后两周采血，分别分离血清和淋巴细胞，用间接免疫荧光试验检测小鼠的体液免疫功能，用淋巴细胞转化实验来检测小鼠的细胞免疫功能。

Two weeks after the third immunization , we separated serum and lymphocytes separately to detect the functions of humoral immunity with indirect immunofluorescence test , and detect the functions of cellular immunity with lymphocyte transformation test .

猪生殖和呼吸综合征间接免疫荧光抗体试验诊断技术的研究

Studies on IFA diagnostic technique for porcine reproductive and respiratory syndrome

间接免疫荧光抗体试验在传染性非典型肺炎诊断中的应用

The application of indirect immuno-fluorescence assay in the diagnosis of severe acute respiratory syndrome

目的：调查山东省沂蒙山区莱姆病自然疫源地。方法：1992～1999年设点，用间接免疫荧光抗体试验进行人群莱姆病感染调查；

From 1992 to 1999 an endemic investigation of Lyme disease was conducted by means of serological and etiological methods in Yimeng mountains of Shandong province .