等电聚焦

微量血清等电聚焦电泳及免疫印迹法测定人载脂蛋白E表型

Phenotyping of human apolipoprotein E from micro-serum by isoelectric focusing and immunoblotting

等电聚焦电泳结果表明该变应原pⅠ为4.8-7.5，在此范围内形成6条蛋白质染色条带，这表明DerfⅠ变应原的不均一性，其组成非常复杂。

By isoelectric focusing , it was revealed to have pIs of 4 . 8 - 7 . 5 with six protein bands , suggesting that Der f ⅰ is heterogeneous with complicated composition .

用超薄等电聚焦检测成都地区人转铁蛋白C亚型和D变异型

Transferrin C Subtypes and D Variants in Chinese by Ultrathin Layer Isoelectric Focusing

固相pH梯度等电聚焦是国际上80年代的新型电泳技术。

The mentioned immobilized PH gradients iso-electrofocusing is a electrophoresis techniquedeveloped in80 's.

基于固定化pH梯度整体材料的芯片自由流等电聚焦电泳模式的构建

Microchip free flow isoelectric focusing with immobilized pH gradient on monolithic materials

一维固相pH梯度等电聚焦与生物质谱联用鉴定混合蛋白质的方法建立与应用

Strategy for Protein Mixture Identification by Immobilized pH Gradients Isoelectric Focusing and Mass Spectrometry

天牛能否自身消化植物的纤维素，是长期来争论未决的问题，本课题用聚丙烯酰胺凝胶等电聚焦电泳方法着重研究了纤维素酶复合酶中CX酶在虫体中的来源。

The origin of the chief cellulose digesting enzyme , C_X-Cellulase , in longhorn beetles was investigated .

经等电聚焦电冰分析，等电点（PI）为5.3。

The PI value of 5.3 was determined by iso-electric focusing .

本文报导了用水平等电聚焦电泳技术由粗酶液一步纯化制得纯青霉素G酰化酶。

Penicillin G acylase enzyme of E. coli was purified in one step by horizontal preparative electrofocusing .

用等电聚焦酶免疫分析法检测人类精斑GC表现型

Phenotyping of GC in human semen stains using IEF followed by enzyme immunoassay

等电聚焦法检测人类血痕中的Pi亚型

Subtyping of PI in human bloodstains by isoelectric focusing

用等电聚焦免疫印迹法测精液（斑）、阴道液以及混合斑中Tf的亚型

Transferrin Subtyping of Human Semen , Semen Stain , Vaginal Fluid and Mixed Stain Using Isoelectric Focusing and Immunoblotting

本文以细胞色素C为标样对二维毛细管等电聚焦和毛细管区带电泳的分离性能进行了考察，同时对肺癌细胞的分泌蛋白进行了分离分析。

The Chrome C sample and proteins excreted from lung cancer cells were selected to demonstrate the interface 's feasibility and resolving performance in2D CE system .

等电聚焦表明，A、B两组分的等电点分别为7.2和6.9。

And the isoelectric points ( pI ) of the two SOD components ( A and B ) were 7.2 and 6.9 respectively by isoelectric focusing .

用等电聚焦免疫固定技术调查成都地区汉族群体Bf的遗传多态性。遗传算法及其在排班问题中的应用

Genetic algorithms and its application in shifts arrangement

用三维试验检测重组菌产β-内酰胺酶的表型，等电聚焦电泳测定其表达蛋白的等电点（pI）。

The isoelectric point ( pI ) of the recombinant protein was determined by isoelectric focus electrophoresis .

牦牛血红细胞CuZn-SOD的薄层等电聚焦电泳行为分析

Thin-layer isoelectrofocusing electrophoretic analysis of Cu , Zn-SOD from red blood cells of yaks

经旋转等电聚焦电泳，一步纯化31.75倍，回收率为56.25%，比活性达1.02×10~7U／mg蛋白。

75 times with a recovery rate of 56 . 25 % and the specific activity of 1 . 02 X 107U / ml protein .

纯化的AB片段分子量为98000，于等电聚焦中为2条主要区带，pI为5.3和5.5，另有数条小区带；

Fragment AB was focused as two major bands with pIs of 5.3 and 5.5 and several faint bands .

等电聚焦电泳结果显示ES抗原有22条带，几乎全部是酸性蛋白，pI主要集中在4.25～5.25范围内；

IEF indicated that ES antigens had 22 bands , mainly consisted of acidic proteins ( pI 4 2 5.25 );

等电聚焦证明其等电点（pI）为4.8.吸收光谱及荧光光谱同已有报道。

The isoelectric point was 4.8 by isoelectric focusing . The absorption and fluorescence spectrum of PC were similar to regular reports .

人血清apoE表型等电聚焦免疫印迹分析法

Isoelectric Focusing and Immunoblotting Identification of Human ApoE Phenotype

血清磷酸肌酸激酶BB的薄层等电聚焦分析法

Analysis of Plasma Creatine Kinase - BB by IEF - PAGE

采用RAPD技术和等电聚焦电泳方法，对福建棘隙吸虫福建株和广东株虫体进行基因组DNA多态性分析和蛋白质等电点测定。

By using RAPD technique and isoelectric focusing electrophoresis method , genomic DNA and protein from Guangdong strain and Fujian strain of Echinochasmus fujianensis were analysed respectively .

这4个突变体对于改良水稻谷蛋白品质、研究谷蛋白生物合成遗传调控机制以及揭示谷蛋白基因功能是不可多得的研究材料。用等电聚焦免疫固定技术调查成都地区汉族群体Bf的遗传多态性。

The mutants are considered to be useful material for glutelin quality improvement and for genetic regulatory mechanism study of glutelin biosynthesis .

台布一直垂到地板上。人血清apoE表型等电聚焦免疫印迹分析法

The tablecloth trails on the floor . Isoelectric Focusing and Immunoblotting Identification of Human ApoE Phenotype

新种质，并用减数分裂配对分析、单体小麦测交法、基因组原位杂交、C分带、同工酶等电聚焦和RAPD对上述材料进行了综合鉴定；

The six germplasms were comprehensively identified by meiosis pairing analysis , testing cross analysis using monosomic analysis , C_banding , isoelectrofocusing ( IEF ) of isoenzyme and RAPD analyses .

用等电聚焦（IEF）法初步分析了4例献血员丙型肝炎病毒（HCV）感染血清。

Hepatitic C virus ( HCV ) serum from four male blood donors were analysed with IEF electrophoresis .

本实验的目的是采用载体两性电解质pH梯度等电聚焦电泳的方法，结合SDS-PAGE测定牛凝血酶的等电点。

The purpose of the experiment is to assay the isoelectric point of bovine thrombin by carrier ampholytes pH gradient isoelectrofocusing ( IEF ) electrophoresis and SDS-PAGE .

样品浓缩除盐后，进行一维等电聚焦分离和二维SDS电泳分离提取人肾组织磷酸化蛋白。

After being concentrated and desalted , the samples were separated by isoelectric focusing on first dimension and SDS electrophoresis on second dimension .