等电聚焦电泳

采用RAPD技术和等电聚焦电泳方法，对福建棘隙吸虫福建株和广东株虫体进行基因组DNA多态性分析和蛋白质等电点测定。

By using RAPD technique and isoelectric focusing electrophoresis method , genomic DNA and protein from Guangdong strain and Fujian strain of Echinochasmus fujianensis were analysed respectively .

猪囊尾蚴蛋白质的聚丙烯酰胺凝胶电泳和等电聚焦电泳分析

Protein analysis of Cysticercus cellulosae with polyacrylamide gel and isoelectric focusing electrophoresis

基于固定化pH梯度整体材料的芯片自由流等电聚焦电泳模式的构建

Microchip free flow isoelectric focusing with immobilized pH gradient on monolithic materials

微量血清等电聚焦电泳及免疫印迹法测定人载脂蛋白E表型

Phenotyping of human apolipoprotein E from micro-serum by isoelectric focusing and immunoblotting

天牛能否自身消化植物的纤维素，是长期来争论未决的问题，本课题用聚丙烯酰胺凝胶等电聚焦电泳方法着重研究了纤维素酶复合酶中CX酶在虫体中的来源。

The origin of the chief cellulose digesting enzyme , C_X-Cellulase , in longhorn beetles was investigated .

本文报导了用水平等电聚焦电泳技术由粗酶液一步纯化制得纯青霉素G酰化酶。

Penicillin G acylase enzyme of E. coli was purified in one step by horizontal preparative electrofocusing .

用三维试验检测重组菌产β-内酰胺酶的表型，等电聚焦电泳测定其表达蛋白的等电点（pI）。

The isoelectric point ( pI ) of the recombinant protein was determined by isoelectric focus electrophoresis .

牦牛血红细胞CuZn-SOD的薄层等电聚焦电泳行为分析

Thin-layer isoelectrofocusing electrophoretic analysis of Cu , Zn-SOD from red blood cells of yaks

经旋转等电聚焦电泳，一步纯化31.75倍，回收率为56.25%，比活性达1.02×10~7U／mg蛋白。

75 times with a recovery rate of 56 . 25 % and the specific activity of 1 . 02 X 107U / ml protein .

等电聚焦电泳结果显示ES抗原有22条带，几乎全部是酸性蛋白，pI主要集中在4.25～5.25范围内；

IEF indicated that ES antigens had 22 bands , mainly consisted of acidic proteins ( pI 4 2 5.25 );

本实验的目的是采用载体两性电解质pH梯度等电聚焦电泳的方法，结合SDS-PAGE测定牛凝血酶的等电点。

The purpose of the experiment is to assay the isoelectric point of bovine thrombin by carrier ampholytes pH gradient isoelectrofocusing ( IEF ) electrophoresis and SDS-PAGE .

通过等电聚焦电泳和接合试验分析ESBLs的表型特征；

Isoelectric focusing overlay technique and conjugation experiments were used to find ESBLs and determine the phenotypical characteristics of ESBLs .

总蛋白的等电聚焦电泳（IFE）和过氧化物酶同工酶的聚丙烯酰胺凝胶电泳（PAGE）分析的结果，均与受体不同，而且过氧化物酶的酶活性显著地大于受体的酶活性。

Zymograms of total protein IFE and PAGE of the Peroxidase isozyme were obviously different from the acceptor , and the peroxidase activity was significantly increased .

超薄平板等电聚焦电泳薄栅氧化层的TDDB研究

SUPER - THIN LAYER ELECTROFOCUSING Research on TDDB of thin gate oxide

等电聚焦电泳结果表明该变应原pⅠ为4.8-7.5，在此范围内形成6条蛋白质染色条带，这表明DerfⅠ变应原的不均一性，其组成非常复杂。

By isoelectric focusing , it was revealed to have pIs of 4 . 8 - 7 . 5 with six protein bands , suggesting that Der f ⅰ is heterogeneous with complicated composition .

从中国湖北建始和利川毛坝生漆中提取粗漆酶，应用等电聚焦电泳（IEF）技术分离鉴定同工酶。

The isoenzymes in coarse laccase by extraction from raw Chinese lacquers were separated and identified by isoelectrofocusing .

毛细管等电聚焦电泳定量分析健康成人及地中海贫血携带者的HbA2

Use of capillary electrophoresis to determine hemoglobin A_2 in healthy adults and α - and β - thalassemia carriers

采用聚丙烯酰胺等电聚焦电泳（IEF）方法，比较了10株糙皮侧耳菌漆酶同工酶谱。

Comparison of laccase isozymes among ten strains of pleurotus ostreatus was made by using isoelectric focus ( IEF ) .

毛细管等电聚焦电泳（cIEF）及其理论模拟计算新进展

Progress in capillary isoelectric focusing ( cIEF ) and theoretical simulation of its process

采用超薄聚丙烯酰胺平板凝胶等电聚焦电泳和单株后代法，分析了栗属种的PGI同工酶的遗传。

Inheritance of PGI isozymes in chestnut species was analyzed using isoelectric focusing on thin layer polyacrylamide slab gels and single tree progeny method .

目的：探讨利用种子贮藏蛋白SDS&PAGE电泳、酯酶同工酶超薄等电聚焦电泳和RAPD分子标记鉴定五个两系杂交水稻组合F1种子纯度的可行性。

Objective : To test the F_1 genetic purity of five two-line hybrid rice combinations , SDS-PAGE of seed storage proteins , ultrathin - layer isoelectric focusing of esterase isoenzyme and RAPD molecular markers were used in this report .

分别用SDS-聚丙烯酰胺凝胶电泳法（SDS-PAGE）及非变性孔径梯度聚丙烯酰胺凝胶电泳法测定其分子质量，用聚丙烯酰胺管状等电聚焦电泳法测其等电点。

Its molecular mass was measured by SDS - polyacrylamide gel electrophoresis ( SDS-PAGE ) and native pore-gradient polyacrylamide gel electrophoresis respectively . Its isoelectric point ( pI ) was determined by isoelectric focusing tube electrophoresis .

聚丙烯酰胺凝胶等电聚焦电泳呈现五条带，主带在pH7.8处；DNS法测定其N-末端氨基酸为苯丙氨酸一种。

In isoelectric focusing five bands were found and the major band was at pH 7.8.The N-terminal amino acid determined by the dansyl method was phenylalanine .

用SDS不连续聚丙烯酰胺凝胶板电泳、pH4.5不连续聚丙烯酰胺凝胶板电泳和聚丙烯酰胺凝胶圆盘等电聚焦电泳，分离我国不同地区东亚钳蝎毒。

The scorpion venoms of Buthus martensii Karsch from 3 regions in China were separated by SDS-polyacrylamide gel slab electrophoresis , pH4 . 5-polyacrylamide gel slab electrophoresis and iso-electric focusing electrophoresis .

等电聚焦电泳表明该种鱼GH由等电点分别为6.3和6.6的两种形式的分子组成。

Isoelectric focussing showed that sGH consisted of two protein bands having isoelectric points ( PI ) of 6.6 and 6.3 , respectively . These values has been compared to the corresponding data of GHs from other fishes .

在游离与结合配基分离方面，除建立与比较了DCC（葡聚糖包埋的活性炭）及HAP（羟基磷灰石）方法外，还首次将IEF（等电聚焦电泳）应用于DHCCR分离技术。

In separating free and combined ligands , the IEF ( Isoelectric Focusing ) technique was first used besides establishing and comparing these two methods of DCC ( Dextran Coated Charcoal ) and HAP ( Hydroxyapatite ) .

从酯酶同工酶等电聚焦电泳得出，ABM-R种群的E7、E13和E15同工酶活力显著提高是导致ABM-R种群酯酶活力提高的主要原因。

Esterase isozyme upon isoelectric focusing eletrophores demonstrated that the high levels of total esterase activity in ABM R was due to the increased activities of E7 , E13 and E15 isozymes .

本文用0.5mm薄层等电聚焦电泳技术对三种马鸡的卵清蛋白进行了比较分析。

In the present study a comparative analysis of the egg white proteins among three species of the genus Crossoptilon was conducted by using 0.5 mm thin layer gel electro-focusing and gel scanning .

三种绦虫蛋白质等电聚焦电泳比较分析

Comparative electrophoretic protein analysis of three species of cestodes by isoelectric focusing

基因工程人β干扰素的旋转等电聚焦电泳分离

Separation of Recombinant Human Interferon - β by Rotary Isoelectric Focusing Electrophoresis