吸附层析法

阶段洗脱吸附层析法分离茶多酚咖啡碱

Stepwise elution adsorption chromatography separation for tea polyphenols and caffeine

钙调蛋白分离纯化的吸附层析法吸附层析法制备低聚原花青素

Preparation of oligomeric proanthocyanidins by adsorption chromatography

吸附层析法从啤酒废酵母中提取谷胱甘肽

Separation Glutathione from Abandoned Beer Yeast by Absorption Chromatography

酶Ⅱ最后再采用羟基磷灰石吸附层析法纯化，最终纯化倍数为20.68倍，回收率为38.53%。

Alliinase ⅱ was purified up to 20.68 times with a recovery of 38.53 % .

方法应用聚酰胺吸附层析法，从香山绿茶的水提取物中分离精制茶多酚。

Method The purified tea polyphenol was extracted from xiangshan green tea with polyamide chromatography .

结果聚酰胺吸附层析法提取的茶多酚纯度高（96%），产量约15%。

Results The tea polyphenol extracted with polyamide chromatography possessed higher purity ( 96 % ) and product ( about 15 % ) .

笔者采用大孔吸附树脂层析法从茶中分离，纯化茶多酚，制得低含咖啡碱的茶多酚粗制品，得率为18%～21%，纯度≥70%，EGCG＞20%，咖啡碱＜2%。

In present paper , the authors have prepared TPs products which have little caffeine by using macroporous adsorption resin to extract and purify . The product rate is 18 % - 21 % 3 the purity > 70 % . EGCG > 20 % and caffeine < 2 % .

采用国产LSA-10型大孔吸附树脂层析法对山楂原花青素进行分离纯化，在梯度洗脱中，体积分数为50%乙醇溶液的洗脱能力最强，洗脱液中原花青素的干基纯度达83.2%。

The extraction was purified by LSA-10 macroporous adsorption resin . The step-gradient elution was conducted and the fraction obtained by 50 % alcohol elution contained the most procyanidins up to 83.2 % .

研究了离子沉淀法和大孔吸附树脂层析法结合制备茶多酚的工艺。

Combining the ion precipitation with column chromatography to prepare tea polyphenol was studied .

采用配位吸附柱层析法分离纯化市售粉末大豆磷脂（磷脂酰胆碱含量40%），得到高纯度卵磷脂产品（磷脂酰胆碱含量96.2%）。

Phosphatidyl choline of high purity ( PC content 96.2 % ) was prepared from commerial soybean powder phospholipids ( PC content 40.0 % ) by coordination adsorption column chromatography ( CACC ) .

采用H-600树脂吸附阶段洗脱层析法分离茶多酚咖啡碱，解决了传统工艺中使用有毒溶剂脱除咖啡碱的难题。

By using H-600 resin adsorption and stepwise elution chromatography process for tea-polyphenols and caffeine separation , this process can solve the problem in conventional process in which caffeine must be eliminated with poisonous solvent .

细菌免疫吸附柱亲和层析法提取抗菌抗体的研究

Purification of antibacterial antibodies with the bacterial immunoabsorbent column affinity chromatography

采用大孔吸附树脂及凝胶层析法能够较好地分离纯化燕麦ACE抑制肽。

Results indicated that macroporous resin and gel filtration chromatography could better purify ACE inhibitory peptides from oat .