凝胶过滤层析

经凝胶过滤层析和非还原SDS-PAGE分析，验证了其确系三聚体形式。

The gel filtration chromatography and non-reduced SDS-PAGE identified the trimeric structure of the recombinant protein .

应用SDSpage和凝胶过滤层析对分子量的测定，提示该酶是由4个分子量为110～115kD的相同亚基组成的同源四聚体。

It was estimated that the purified hydrolase was consisted of four identical subunits with a molecular mass of 110 ~ 115kD by SDS-PAGE and gel filtration chromatography .

本文应用超速离心、凝胶过滤层析、高效液相色谱法分离纯化了人血清极低密度脂蛋白&载脂蛋白CⅠ和CⅢ。

Apolipoprotein C ⅰ, C ⅲ were isolated and purified by ultracentrifugation , gel filtration and high performance liquide chromatography from human serum very low density lipoprotein .

方法：利用亲合层析及凝胶过滤层析从小鼠黑色素瘤中提取小鼠黑色素瘤凝集素（MMA）。

Methods : MMA was prepared with asialo-fetuin affinity and gel-filtration chromatography form murine melanoma .

经SDS-PAGE（12%）和凝胶过滤层析，测得其分子量约为52.0～53.6kD。

Through SDS-PAGE and gel filtration , the molecular weight of the purified P - 1,3-glucanase was determined to be about 52.0-53.6 kD .

本研究没有对热稳定的溶血素进行提纯分析，而对热不稳定的溶血素经过盐析及凝胶过滤层析提纯后，进行SDSPAGE分析，表明该溶血素为分子量65kDa的蛋白。

The former has not been studied but the latter did . SDS_PAGE analysis following purification by ammonium sulfate precipitation and filtration chromatography showed that the heat_ labile hemolysin is a 65_Kilodalton protein .

采用ICPMS、凝胶过滤层析及超滤技术，通过动物实验研究了长时间、低剂量镧作用后，镧在Wistar大鼠肝脏中的代谢累积及其物种分布。

The metabolic accumulation and species of lanthanum in Wistar rat liver were investigated by ICP MS , gel exclusion chromatography and ultrafiltration after the rats were fed by low dose of lanthanum for a long time .

经过阴离子交换层析和凝胶过滤层析两步纯化，SRH纯度达95%以上。

The purity of SRH reached to 95 % after two steps of purification with anion-exchange chromatography and gel filtration .

结论：运用假配基亲和层析和凝胶过滤层析相结合的手段提取、纯化苦瓜RIP是可行的；苦瓜RIP可能是通过诱导细胞发生凋亡来发挥其抗肿瘤活性。

Conclusion : The methods presented in this paper are feasible to isolate and purify the RIP and the RIP extracted from bitter gourd may exert its anti-tumor activity through induction of apoptosis .

经SDS-PAGE和凝胶过滤层析测得该纯化酶的分子质量分别为42ku和40ku，表明该酶以单体形式存在；

The molecular mass of the purified enzyme was estimated to be 40 ku by Sephadex G-75 gel filtration and 42 ku by SDS-PAGE , which indicated that the β - mannanase was a monomer .

经CM柱阴离子交换层析和Sephacryls-200凝胶过滤层析后的样品，初步测定其分子量约为31800（31.8KD）。

Preliminary testing to the sample after CM anion exchange chromatography and Sephacryl S-200 gel filtration , showed its molecular weight is approximately 31800 ( 31.8KD ) .

结果两步亲和层析结合凝胶过滤层析有效地分离纯化了大鼠β2GP1，蛋白纯度达90.78%；

RESULTS The rat β 2GP1 was isolated and purified effectively , the purity was 90.78 % .

包涵体经过洗涤、裂解、蛋白质复性以及SephadexG100凝胶过滤层析等步骤纯化后，获得了纯度约92.6%的人血管能抑素N端片段，CAM实验证明具有显著抑制鸡胚新生血管生成活性。

The inclusion bodies were washed , lysed and the reactivated proteins were purified by the Sephadex G-100 gel filtration to a purity of 92.6 % . CAM assay showed that N-domain effectively inhibited the angiogenesis of chichen embryo microcapillary vessel .

为研究LDL的理化性质及其功能，研究人员发明了多种LDL分离技术，如超速离心、化学沉淀、凝胶过滤层析及亲和层析等，但这些技术均有不足。

In order to study the physical-chemical properties and functions of LDL researchers have invented a variety of LDL separation methods , such as ultracentrifugation , chemical precipitation , gel filtration and affinity chromatography . However , each of them has its disadvantages .

经过硫酸铵分级沉淀、SephadexG-100凝胶过滤层析和DEAE-sePharoseFF离子交换层析对大麦虫水溶性蛋白逐步分离纯化，并对所得的各组分的抗氧化性进行研究。

The water dissolved protein of barley pest was extracted by means of salt solution and was purified by ammonium sulfate precipitation with Sephadex G-100 gel filtration and DEAE-Sepharose FF chromatography . The antioxidant activity of each component was also investigated .

该酶经过两次硫酸铵盐析、DEAE-52离子交换层析和SephadexG-200凝胶过滤层析后，其比活力由21.5U/mg提高到5944.1U/mg，纯化278.5倍，回收率为17.8%，达到电泳纯。

The BChE was purified to electrophoretic homogeneity by salting out , ion-exchange chromatography on DEAE-cellulose and gel filtration on Sephadex G-200 . Finally the enzyme was purified 278 . 5 folds and the enzyme activity recovery ratio was 17.8 % .

方法新鲜牛晶状体匀浆与[14C]果糖孵育24h，凝胶过滤层析（Sephacryls300HR）分离水溶性蛋白质，并进一步分离脱氢酶；

Methods The fresh bovine lens homogenate was incubated with [ ~ 14C ] fructose for 24 hours . The water-soluble proteins were separated by size-exclusion chromatography ( Sephacryl S-300HR ) and then were passed over an enzyme column to separate out the dehydrogenases .

芽孢杆菌2（Bs-2）的发酵液经过硫酸铵分级沉淀、SephadexG-75凝胶过滤层析、Q-Sepharose阴离子交换层析、电泳制备和电洗脱，获得一种具有纤溶活性的蛋白酶；

A fibrinolytic enzyme was purified from the crude extract of Bs-2 , a strain of Bacillus sp . , through ammonium sulphate fractional precipitation , Sephadex G-75 gel filtration , Q-Sepharose anion exchange chromatography , preparative PAGE and electric elution .

凝胶过滤层析分离纯化纤维素酶的研究

Separation and Purification of Cellulase by Column Chromatography of the Gel Filtration

组织培养技术检测产物的生物学活性并计算纯化得率。结果：纯化后的病毒经凝胶过滤层析分析仅见一个层析峰；

Results : The gel filtration analysis of purified virus showed only one absorbance peak ;

通过亲和层析技术和凝胶过滤层析技术来进行后续的纯化工作。

Purification of recombinant proteins were performed by MBP affinity chromatography and gel filtration chromatography .

凝胶过滤层析参数对家蝇蛋白粗提液分离效果的影响

Optimization of the gel-filtration chromatography parameters for separating proteins from the crude extracts of housefly

凝胶过滤层析分离纤维低聚糖

Isolation of Cellooligosaccharides by Gel Filtration

批量离子交换层析和凝胶过滤层析在类人胶原蛋白I纯化中的应用

Purification of human-like collagen biopolymer I by batch ion exchange chromatography combined with gel filtration chromatography

凝胶过滤层析；

Gelatin filtration chromatographic analysis ;

方法采用凝胶过滤层析技术对针刺全血清进行分段分离，并将分离的血清经冷冻干燥制成干粉。

Method The acupuncture serum were separated by gel filtration with Sephadex G 75 and molecular standards .

凝胶过滤层析分离蛋白质实验中三种过滤介质分离效果的比较

The observation of separating effects on three kinds of gel-filtration chromatography media to separate the protein mixture

目的探讨凝胶过滤层析分离蛋白质的最佳实验效果。

Objective To find out the optimal condition for separation of the protein mixture by gel filtration chromatography .

本实验利用饱和硫酸铵盐析和葡聚糖凝胶过滤层析，从猪视网膜中提取了S抗原。

The soluble antigen was isolated from pig retinal extract by precipitation with ammonium sulfate followed by molecular sieve .

研究了利用批量离子交换层析与凝胶过滤层析相结合的方法纯化类人胶原蛋白I的最佳条件。

The method of batch ion exchange chromatography combined with gel filtration chromatography to purify Human-like Collagen Biopolymer I was investigated .