核移植

并检测了该该基因调节区在体外受精胚胎、孤雌胚胎、核移植胚胎中的DNA甲基化状态。

The DNA methylation status in IVF embryos , parthenogenetic embryos , and nuclear transfer embryos were also analyzed .

方法取昆明小鼠胚胎分离成纤维细胞，用丝裂霉素C处理成纤维细胞为胚胎干细胞制备饲养层，消化贴壁成纤维细胞为核移植提供供体细胞。

Mitomycin C was used to pretreat MEF for feeder layers of embryonic stem cell and MEF was digested for donor cells of nuclear transfer .

维生素E对牛核移植供体细胞凋亡率的影响

The Effect of Vitamin E On Apoptosis Rate of Bovine Donor Cell for Nuclear Transplantation

这表明印迹基因在核移植胚胎中的去甲基化是由DNA复制过程中维持甲基化的失败造成的。

The results suggest that demethylation of methylation imprints in NT embryos is caused by failure of maintence methylation during DNA replication .

可以利用核移植技术建立核移植胚胎干细胞（nucleartransferembryonicstemcells.ntES）系，利用胚胎干细胞制备嵌合体动物，对胚胎干细胞进行基因改造等研究。

Nucleus transplantation technology could make nuclear transfer embryonic stem cell lines . Using ES cells could make chimera animals and modify genes , etc.

脂质体介导的鲫CAB细胞转化及转化细胞的核移植

Lipofection of cells of CAB strain of Carassius auratus and nuclear transplantation of transformed cells

利用IVF废弃胚胎为核移植受体构建人体细胞克隆胚胎

Production human somatic cell nuclear transfer embryos using IVF discarded embryos

牛体细胞克隆胚胎类ES细胞集落的筛选及其核移植

Isolation and Nuclear Transfer of ES-like Cells Colonies Derived from Embryos being Cloning of Bovine Somatic

9个核移植重构囊胚中分离出ES细胞集落，分离率为2.77%；

ES cell colonies were isolated from 9 blastocysts reconstructed , isolation rate was 2.77 % .

目的：研究SD大鼠成纤维细胞同步处理后的各细胞周期的变化，探讨供体细胞的细胞周期对核移植胚胎发育的影响。

To investigate the cell cycle characteristics of SD rat fibroblast cells cultured under starvation of nocodazole synchronization treatment .

克隆这个词最初是应用于园艺学，随着克隆技术的发展，克隆概念不断获得新的含义，扩展到了细胞培养技术、核移植技术、以及DNA序列的扩增技术。

Its implication of the term " clone " has been renewed continuously , extending to the techniques of cell culture , nuclear transplant , and multiplication of DNA sequences .

整合人lactoferrin基因的山羊体细胞支持核移植克隆胚的体外发育

Nuclear Transfer of Goat Somatic Cells Transgenic for Human Lactoferrin

山羊胎儿成纤维细胞转染人t-PA指形区缺失基因及其核移植研究

Study of transfer t-PA gene to the goat fetal fibroblasts and nuclear transfer

TACE在自体髓核移植背根节致根性神经痛中的作用机制研究

Study of TACE in the mechanism of hyperalgesia derived by auto-transplantation of nucleus pulposus to DRGs

曲古抑菌素A对核移植供体细胞组蛋白H3乙酰化水平的影响

The Effect of Trichostatin A upon Histone H3 Acetylation in Nuclear Transplantation Donor Cells

探讨TSA不同处理时间对核移植重构胚的卵裂率和囊胚率的影响。

Investigated the effect of TSA different treatment time on the nuclear transfer embryo cleavage and blastocyst rate .

目前还没有人知道是否能够利用iPS细胞通过核移植的方法获得克隆动物。

However , it remains unknown whether iPSCs could be used for generating cloned animals through nuclear transfer method .

结论注射HCG后16h的卵母细胞为适宜的小鼠核移植的受体细胞。

Conclusion The oocytes 16 h after HCG injection was the ideal recipient cell used for somatic cloning .

通过G418筛选细胞，为本实验室利用核移植技术生产转基因动物提供细胞来源。

So the cells can be used as sources to create transgenic animal by nuclear transplant techniques .

5-Aza-CdR联合TSA对牛核移植胚胎体外发育及表观遗传状态的影响

Effect of 5-Aza-CdR and TSA on the in vitro development of bovine cloned embryos and epigenetic status

用注射LH后25-35小时的次级卵母细胞作胞质供体均可获得较高的卵核移植胚体外发育率；

Higher survival rate of nuclear transplanted embryos in vitro can be obtained when secondary oocytes collected at 25-35 h after LH injection were used as cytoplast donors ;

为优化山羊核移植方案，实验研究了细胞松弛素B（cytochalasin-B，CB）处理和胞质去除对山羊孤雌胚体外发育的影响。

To optimize the protocols of nuclear transfer , effects of cytochalasin-B ( CB ) treatment and cytoplasm removal on parthenogenetic embryos of goat were observed .

一般认为核移植胚的正常发育取决于供体核处于细胞周期的G0期或G1期。

Normal development of nuclear transfer embryos was thought to be dependent on transferral of nuclei in G0 or G1 phases of the cell cycle .

为结合核移植技术，制备高表达IL-11的转基因山羊乳腺生物反应器，我们开展了胚胎成纤维细胞基因打靶相关研究。

To generate IL-11 high-expression transgenic livestock , many researches were carried out on embryonic fibroblast gene-targeting assay .

CNS脱髓鞘疾病生物标记物的蛋白质组学研究自体髓核移植可致脊神经节、神经根明显的水肿、炎性反应、脱髓鞘等病理改变。

Proteome Analysis of Biomarkers in the Cerebrospinal Fluid of Central Nervous System Demyelinative Diseases Significant endoneurial edema , inflammation reaction and demyelination can be seen in the DRGs and NRs .

为更好地了解该领域当前的进展，我们研读了相关的文献，包括核移植过程中核质互作、核重编程、线粒体命运、端粒变化以及X染色体失活等机理方面的著述。

For better understanding of current progress in this field , we gathered reports on molecular ways affecting the success of animal cloning , such as nuclear cytoplasm interaction , epigenetic reprogramming , mitochondrial fate , telomere changing and X chromosome inactivation , etc.

在过去的20年里，出现了一些新的转基因方法，包括精子介导、反转录病毒介导、携带外源基因体细胞的核移植、ES细胞基因打靶技术等。

In the past 20 years , some new approaches have been used . These include sperm mediated DNA transfer , retroviral mediated DNA transfer into oocytes , somatic cell carrying exogenous genes nuclear transfer , the use of embryonic stem ( ES ) cell .

为了给兔核移植研究提供最佳的兔卵电激活条件，研究了影响电刺激诱导兔卵孤雌活化的3个因素：细胞松弛素（CB）对卵的预处理，电极距离及脉冲强度。

Three factors , cytochalasin B ( CB ) pre - treatment , width of two electrodes and field strength of electric pulse ( direct current ) related to the parthenogenetic activation of rabbit eggs by electric stimulation were studied for nuclear transplantation .

本实验旨在探索水牛生发泡（GV）期卵母细胞程序化冷冻保存的条件，希望能提高其保护效果，为水牛的IVM、IVF、核移植等生物技术提供实验材料。

This study was to explore program freezing conditions for buffalo GV oocytes , aiming at improving protective effect of program freezing , to provide experimental materials for buffalo 's IVM , IVF , nuclear transfer and other biotechnologies .

TNF-α及TNF-RⅠ对自体髓核移植背根节所致痛觉过敏的调控研究

The study of TNF - α and TNF-R ⅰ in modulation of hyperalgesia derived by auto-transplantation of nucleus pulposus to DRGs