甲苯胺蓝

方法采用甲苯胺蓝染色和免疫组织化学ABC方法。

Methods Using toluidine blue stains and immunohistochemical ABC methods .

在常规甲苯胺蓝染色法中，MC不易定位，被染成蓝色。

But with normal toluidine blue , the MC appeared blue and could not be located easily .

本文用因子Ⅷ相关抗原免疫组化（S－P）方法观察了转移及非转移鼻咽癌组织的微血管，并用甲苯胺蓝法观察了其肥大细胞数量。

Microvessel and mast cell , were studied by immunohistochemistry using antibody to factor ⅷ related antigen and toluidine blue stain in 30 cases of nasopharyngeal carcinoma .

在快速甲苯胺蓝染色法中，组织结构清晰可辨，MC易定位，呈紫红色；

The structure was distinctive with fast toluidine blue , the MC appeared purple were easy to locate .

诱导21天甲苯胺蓝染色阳性，特异性表达II型胶原。

After 21 days of induction , the cells were positively stained by toluidine blue , and immunohistochemical analysis proved type II collagen expression strongly .

本实验方案采用MTT、甲苯胺蓝、H&E染色对此进行了评价。

In this study , MTT , toluidine blue , H & E staining has been used as evaluation methods .

采用甲苯胺蓝染色和免疫组织化学ABC方法，对大鼠舌组织中肥大细胞的分布、形态特点以及细胞内的P物质、血管活性肠肽和神经肽Y的定位进行了研究。

Using toluidine blue staining and immunohistochemical ABC method we studied the distribution of SP , VIP and NPY in rat tongue mast cells and their morphologic characteristics .

He-Ne激光诱导甲苯胺蓝增感丙烯酰胺乙二醇溶液聚合动力学

The Polymerization Kinetics of Acrylamide in Glycol Solution Induced by He-Ne Laser with Toluidine Blue as the Sensitizing System

方法：选择MG患者胸腺切除标本28例，分别行常规病理制片、HE染色、光镜观察和甲苯胺蓝染色后对胸腺中肥大细胞（MC）作半定量分析。

Methods Thymus tissues of 28 patients with MG were examined by light microscopy , and a semi quantitative analysis of the thymus mast cells was made .

方法采用改良甲苯胺蓝染色法检测120例患者胃粘膜中MC计数及脱颗粒细胞所占比例。

Methods The improved toluidine blue pigmentation was used to examine the number of gastric mucosal MC and its degranulation portion of 120 patients .

结果经与甲苯胺蓝邻片对比观察，舌组织内肥大细胞分别呈SP、VIP和NPY免疫反应性；

Result The mast cells in rat tongue showed SP , VIP and NPY immunoreactive by comparing near toluidine blue stains sections .

甲苯胺蓝染色及免疫组化，B组之见少量软骨细胞表达，C组未见软骨细胞表达，细胞外基质与Ⅱ型胶原未见表达。

Through toluidine blue staining and immunohistochemical , little cartilage cells expression is found in group B and no cartilage cells expression in group C , and extracellular matrix and type II collagen were not expressed as well . 4 .

运用甲苯胺蓝染色法观察含药血清对成骨细胞-破骨细胞共育体系中OC形成骨吸收陷窝的影响。

Determine the effect of BBC-containing serum on absorption of lacuna formed by OC in osteoblast and osteoclast co-culture system by toluidine blue staining .

采用水接触角、X射线光电子能谱（XPS）、甲苯胺蓝法（TBO）对所修饰的材料进行了表征。

Surface elemental composition was analyzed qualitatively by X-ray photoelectron spectroscopy ( XPS ) . Surface heparin content was detected by toluidine blue O ( TBO ) .

本研究用HRPSBA标记技术、甲苯胺染色法及甲苯胺蓝和HRPSBA双重染色技术，观察了猪子宫大豆素（SBA）受体细胞和肥大细胞。

SBA receptor cell and mast cell in pig uterus were studied with HRP-SBAmethod , toluidine blue staining and double HRP-SBA-toluidine blue staining .

以甲苯胺蓝为荧光探针，基于DNA对甲苯胺蓝的荧光猝灭作用，建立了一种定量测定DNA的新方法。

A new fluorimetric method has been developed for the determination of deoxyribonucleic acid ( DNA ) with toluidine blue ( TB ) as a fluorescence probe . It is based on the fluorescence quenching of toluidine blue in the presence of DNA .

于缺血不同时限断头取脑，采用免疫组织化学检测Caspase3蛋白表达，HE染色，甲苯胺蓝染色，光镜检查细胞损伤改变，测定丙二醛（MDA）含量。

Caspase 3 protein in rats at different time polits of cerebral ischemia reperfusion was observed by immunohistochemical method , the cell structure was examined under light microscope , and the content of MDA was measured .

采用MTT、甲苯胺蓝染色、VOnKossa染色和RT-PCR分别检测细胞的增殖、生长形态、诱导后细胞的钙化结节和成骨相关基因的表达。

Cell growth , appearance and calcification were detected by MTT , toluidine blue stain and von Kossa stain , respectively . Osteoblast related genes were examined by RT-PCR .

甲苯胺蓝染色阳性率及Ⅱ型胶原基因表达表现为先高后低，实验组与对照组差异有统计学意义（P0.05）。

The positive rate of toluidine staining and the gene expression of collagen ⅱ in the experimental group both increased temporarily and then decreased , but with significant difference between the experimental group and control ( P0.05 ) .

14d后，细胞团经石蜡包埋、切片及HE染色后，进行甲苯胺蓝染色及II型胶原（ColII）的免疫组化染色。

14 days later , micro-cell aggregates formed by the induced MSCs were embedded in paraffin , sectioned and stained with HE , toluidine blue or anti-type II collagen mAb ( Col II ) .

甲苯胺蓝染色细胞核仁发现，正常细胞仅有一个位于中央的、浓染致密的核仁颗粒.过氧化氢处理后3h，可见明显的核仁分离。

Toluidine blue staining showed predominantly compact , centrally localized nucleoli in intact control cells , but in H2O2-treated cells , an early onset of nucleolar segregation could be found after 3 h.

甲苯胺蓝染色结果与Tryptase免疫组化结果相一致，但Tryptase免疫组化方法更为敏感。

The result of toluidine blue staining was consistent with that of tryptase immunohistochemical staining , and the tryptase immunohistochemical method was more sensitive .

方法采用抗Tryptase单抗免疫组化染色法与甲苯胺蓝特殊染色方法，对移植后8d～7a手术切除的排斥肾进行了染色。

Methods The rejected kidneys ( 8 days-7 years after transplantation ) were stained using anti-tryptase monoclonal antibody immunohistochemical method and toluidine blue staining .

结果表明，在Carnoy's氏液或NBF固定的家兔组织均显示出了不同数量的甲苯胺蓝阳性细胞。

Numerous toluidine blue stained mast cells were presented in All samples fixed with Carnoys fluid and NBF .

经成软骨诱导后BMSCs的细胞形态发生变化，由梭形向椭圆形及三角形分化，甲苯胺蓝染色和Ⅱ型胶原染色为阳性。

The cell morphology changed from spindle shape to ellipsoid and triangle shape after BMSCs were induced by specific induction cultural medium . Toluidine Blue staining and type ⅱ collagen staining are both Positive . 4 .

方法收集和固定人滞留乳牙，用甲苯胺蓝和抗酒石酸酸性磷酸酶（TRAP）染色观察和定位破骨细胞，用原位杂交检测降钙素受体mRNA表达情况。

Methods After fixing the collected deciduous teeth , toluidine blue was performed and tartrate-resistant acid phosphatase ( TRAP ) staining was used to identify the osteoclasts on the resorbing surface of human deciduous teeth and in situ hybridization of calcitonin receptor mRNA to show its existence .

方法：Ad-BMP-2转染原代培养的MSC，通过细胞生长曲线、免疫组化、甲苯胺蓝染色透射电镜观察转基因MSC的BMP-2和CollagenⅡ及蛋白多糖表达。

METHODS : MSC was transferred by Ad BMP 2.BMP of the transgeneic MSC , Collagen ⅱ and the expression of proteoglycan were observed by the growth curve of cells , Immunohistochemical method and microscope , Aniline dyeing .

该传感器以甲苯胺蓝键合修饰浸蜡石墨电极为基体电极，将醇脱氢酶、烟酰胺腺嘌呤二核苷酸（NAD＋）同时固定在蚕丝蛋白膜上，成为无试剂的醇传感器。

It is made up of toluidine blue ( TB ) modified , wax-impregnated graphite electrode and enzyme membrane , with alcohol dehydrogenase and nicotinamide adenine dinucleotide ( NAD + ) immobilized on its silk net .

方法：采用甲苯胺蓝快速染色法，对22例ICH、10例体表海绵状血管瘤（SCH，静脉型血管畸形）、9例肉芽肿型血管瘤（GH）组织切片中的肥大细胞进行测定。

Methods : By toluidine blue stain , mast cells in 22 ICH specimens , 10 Superficial Cavernous Hemangiomas ( SCH ) ( Venous malformation ) and 9 Granulomatous Hemangiomas ( GH ) were examined , observed and counted .

利用甲苯胺蓝法对固定后的肝素进行定量，并进行部分凝血活酶时间（APTT）的测定，从而优化出实验所用肝素溶液的浓度。

The amount of heparin immobilized on the surface was determined using the toluidine blue assay . And activated partial thromboplastin time ( APTT ) was tested in order to optimize the concentration of photoactive heparin solution .