嘌呤核苷磷酸化酶

嘌呤核苷磷酸化酶/氟达拉滨（PNP/fludarabine）自杀基因系统对人肝癌细胞系BEL7402体外杀伤效应的研究

Study on Killing Effects of Purine Nucleoside Phosphorylase / Fludarabine ( PNP / fludarabine ) Suicide Gene System on Human Hepatocellular Carcinoma Cell Line BEL7402 in vitro

目的：探讨大肠杆菌嘌呤核苷磷酸化酶自杀基因对胃癌细胞MKN-45的抑制及杀伤作用，并证实旁观者效应。

Objective To study the effects E.coli purine nucleoside phosphorylase suicide gene on human gastric cancer MKN-45 cells as well as the bystander effect .

目的分别构建三种启动子调控的大肠杆菌嘌呤核苷磷酸化酶（PNP）基因表达载体，检测、分析三者的表达差异。

Objective To construct three expression vectors harboring PNP ( purine nucleoside phosphorylase ) gene under three different promoters .

以淋巴细胞减少症和发育缓迟为临床表现的嘌呤核苷磷酸化酶缺陷（PNPdef）1例：经脐带血移植得以成功治疗

Purine nucleoside phosphorylase deficiency ( PNPdef ) presenting with lymphopenia and developmental delay : Successful correction with umbilical cord blood transplantation

基于毛细管电泳在柱酶微反应器技术筛选嘌呤核苷磷酸化酶抑制剂

Purine nucleoside phosphorylase inhibitor screening using immobilized enzyme reactor by capillary electrophoresis

嘌呤核苷磷酸化酶基因的克隆及原核表达载体的构建

Cloning and expression of purine nucleoside phosphorylase in Escherichia coli

γ线对人的红细胞嘌呤核苷磷酸化酶的影响

Effect of gamma irradiation on the activity of human erythrocyte purine nucleoside phosphorylase

γ射线对红细胞嘌呤核苷磷酸化酶的影响二、酶的某些性质

Effect of irradiation on erythrocyte purine nucleoside phosphorylase 2.some properties of the enzyme

合成利巴韦林的关键酶嘌呤核苷磷酸化酶的原核表达及活性分析

Prokaryotic Expression and Functional Analysis of Purine Nucleoside Phosphorylase of the Key Enzyme for Ribavirin Produced

离体与整体照射对大鼠红细胞嘌呤核苷磷酸化酶活力与红细胞膜脂质过氧化值的影响

Effect of gamma irradiation on erythrocyte purine nucleoside phosphorylase activity and erythrocyte membrane lipid peroxidation in vitro and in vivo

嘌呤核苷磷酸化酶是嘌呤核苷补救代谢途径中的关键酶，在抗肿瘤基因治疗、核苷类药物合成等方面具有广泛的应用。

Purine nucleoside phosphorylase ( PNPase , E.C.2.4.2.1 ) is a key enzyme in the purine salvage metabolic pathway . It could catalyze the synthesis of nucleosides , and has great potential in anti-tumour gene therapy .

使用高效液相色谱技术测定嘌呤代谢产物（尿酸、黄嘌呤、次黄嘌呤、次黄苷）及嘌呤核苷磷酸化酶的活性。

The activity of purine nucleoside phosphorylase and release of purine were assayed by HPLC .