标准品

促甲状腺素（TSH）免疫测定用国家标准品的研制

Preparation of TSH national standard for immunoassay

以IAA标准品为对照，来检测海带生长素对微藻生长过程的影响。

In comparison with standard IAA , we detect the influence of extracts from kelp on marine micro-algaes ' growth .

鸟苷酰环化酶C质粒标准品的构建

Construction of the plasmid DNA of guanylyl cyclase - C

乙型肝炎病毒DNA国家定量标准品的研制及初步应用

Preparation and application of the national reference panel for hepatitis B virus DNA

铜绿假单胞菌荧光实时定量PCR标准品的构建

Construction of Reference Standards for Detecting Pseudomonas aeruginosa with Fluorescence Real-time Quantitative PCR

汉坦病毒S片段荧光定量PCR参考标准品的构建

Construction of reference standard plasmids of real-time fluorescence quantitative PCR for detecting Hantavirus S segment

RNA荧光定量标准品制备新方法的探讨

Preparation of RNA Standards for Real-time Fluorescence Quantitative PCR

结果：与原法相比，容易过滤，分离效果好，并与标准品的Rf值一致。

Result . The method is easy to filter and effective to seperation .

大量抽提重组质粒，测定其拷贝浓度，10倍稀释成梯度标准品，并进行荧光定量PCR检测分析。

Standard quantitative curves were constructed by real-time PCR detection with the series of plasmid standards .

结直肠癌患者粪便细菌实时荧光定量PCR标准品制备方法的比较研究

Comparison of standard sample preparation methods with real-time fluorescence quantitative PCR from colorectal cancer patient fecal bacteria

实时荧光定量PCR标准品的制备及应用

The method of preparation for the standard plasmids of real-time PCR and its application in the experiments

人前列腺特异性抗原mRNA实时荧光PCR定量标准品的构建

Construction of the standards for detecting human prostate specific antigen mRNA with real-time fluorescence quantitative polymerase chain reaction

目的：构建增殖诱导配体（APRIL）基因的重组质粒的标准品。

Objective : To construct the standard recombinant plasmids for APRIL gene and using for quantification .

[目的]构建汉坦病毒S片段标准品，用于实时荧光定量PCR检测汉坦病毒。

[ Objective ] To explore the method of preparation for Hantavirus S segment standard plasmids of real-time PCR .

将RNA标准品梯度稀释作为模板进行实时荧光定量PCR反应，制作标准曲线。

The standard curves were made by real-time quantitative PCR reaction using the dilutions of RNA as the template .

阳性结果判断以ACA结合指数上限X标准品A值的数为依据。

Positive results are judged by ACA combination index upper limit standard product A value .

并对合成产物进行IR、NMR和理化性质的测定，结果表明所合成的物质与标准品的数据一致。

The product meet standard material after the comparison of their IR , UV , NMR and properties .

并对2种CHO细胞蛋白标准品和4种供试品进行SDSpage分析。

Two kinds of CHO protein standards and four kinds of samples were analyzed with SDS PAGE .

反应产物经减压精馏、碱洗、水洗、干燥等精制处理，得到了纯度较高的MPC标准品。

The high-purity MPC standard sample was obtained by reduced pressure distillation and the removal of minor phenol with dilute sodium hydroxide solution .

采用chelex100快速抽提DNA，DNA扩增，聚丙烯酰胺凝胶电泳、银染色法，参照标准品进行DNA分型。

The experimental steps include extracting DNA by chelex 100 , amplification of DNA , polyacrylamide gel electrophoresis , silver staining , DNA typing by standard samples .

方法以蛋白质标准品、临床尿液标本为实验对象，通过提高缓冲液的pH值、在缓冲液中加入适当的添加剂等方法，进行芯片蛋白电泳，并与常规琼脂糖电泳结果进行比较。

Methods The standard model proteins and clinical urine specimens were analyzed by improvement of pH and introduction of additive and compared with gelose electrophoresis .

质粒标准品的检测结果证明该方法可对HPV准确分型。

The test results of plasmid standard substance showed that this method can be accurate HPV genotyping .

HCMV质粒标准品的最高检测敏感度为250拷贝／毫升；

The top sensitivity of HCMV standard plasmid was 250 copies / mL ;

HBV-DNA定量标准品的构建

Construction of plasmid standards for quantitation of HBV-DNA

通过以下几种方法对制备的人胰岛素进行鉴定：用PAGE、SDS-PAGE的方法对制备的人胰岛素进行蛋白电泳，均得到与标准品同一位置的单一条带；

This human insulin was identified by the following methods : PAGE 、 SDS-PAGE showed the single lane homogeneous with standard human insulin ;

SDS－聚丙烯酰胺凝胶电泳显示一条带，并与β－乳球蛋白标准品的位置一致。

A band of the purified product on SDS PAGE corresponded with the location of standard β lactoglobulin .

确定了：（1）以剑桥滤片捕集卷烟烟气，标准品对照气质联用法检出A、X主组分的定性分析方法；

The optimum method of qualitative analysis was as follows : using Cambridge filter to collect cigarette smoke , MS / GC was adopted for qualitative analysis .

以盐酸为溶剂，N乙酰基D葡萄糖胺为标准品，用紫外一阶导数光度法直接测定壳聚糖的脱乙酰度。

The degree of deacetylation of chitosan was determined directly by 1st derivative UV spectrophotometry using hydrochloric acid as solvent and N acetyl D glucosamine ( ACGLSM ) as standard .

ELISA比较各检测样品与标准品（ALK）的总生物效价。

The total biologic potency of all the samples was analyzed and compared by ELISA .

具体方法是采用浓度已知的DNA作为标准品建立标准曲线，与未知样本在同一反应板上同时定量，标准曲线为6个点。在建标准曲线同时，确定实时PCR的扩增效率；

The method is : series dilutions of a plasmid with known concentration were used to establish the standard curve and to measure the amplification efficiency of the system .