暗视野

暗视野显微镜在眼科实验研究中的应用

Application of dark field microscope in ophthalmological researches

螺旋体不易观察除非在荧光显微镜或暗视野显微镜观察。

The spirals are not readily seen unless immunofluorescent stain or dark field illumination is employed .

检测了51例临床标本，梅毒螺旋体暗视野显微镜检查阳性检出率为15.7%，双引物PCR阳性检出率为19.6%；

51 clinical samples were detected by dark-field microscopy and a double-primer PCR , the positive rates were 15.7 % and 19.6 % , respectively .

PCR检测结果，与暗视野显微镜检查结果比较，差异有显著性（P<0.01）；

The result of PCR was compared with the results of dark - field microscopy and there was a statistically significant difference ( P < 0.01 ) .

结果复合PCR检测梅毒与暗视野显微镜检查和梅毒血清学检查有较好的一致性。在总计165例患者中，梅毒51例，生殖器疱疹52例，分别占30.9%和31.5%，未发现软下疳病例。

Results There were 51 ( 30.9 % ) cases of syphilis and 52 ( 31.5 % ) cases of genital herpes among 165 patients , respectively . No chancroid case was found .

方法根据病史、临床表现，结合暗视野显微镜检查、梅毒血清学试验，PCR检测HSV-DNA等进行诊断。结果96例女性生殖器溃疡性疾病患者中，梅毒22例，占22.9%；

Methods 96 female patients were examined for their disease history , clinical features , syphilitic serology and HSV-PCR .

〔结果〕在排除了实验室技术因素后，可采用暗视野显微镜检查、梅毒螺旋体IgM抗体检测、荧光梅毒螺旋体抗体吸收试验，对不确定结果进行确认。

Result After eliminating the laboratory technology factor , the methods ( dark-field microscope , the syphilitic spirochaeta ( IgM ) examination , FTA-ABS ) were conducted in order to confirm indeterminate results .

本文介绍了用改良免疫酶染色法，对49例急性钩端螺旋体病人进行检查的结果，并与改良Blenden染色法和暗视野镜检法作了比较。

This paper describes the detection results of 49 cases of acute leptospirosis by modified enzyme labelled antibody staining technique which was compared with the improved Blenden staining method and the dark-field illumination .

用暗视野显微镜对龈下菌斑的初步观察

Preliminary survey on subgingival plaque by darkfield microscopy

研究精子特性的多次曝光暗视野显微摄影装置

Study on the appartus of multiexposure dark-field microphotography of the characteristics about animal spermatozoa

正确的诊断有赖于对可疑患儿的皮疹、胎盘或脐带的渗出物等进行涂片，在暗视野显微镜下用美蓝、嗜银等染色后可检查到梅毒螺旋体。

Pathological diagnosis bases mainly on the finding of spirochete in exudates of exanthema , placenta , or umbilical cord .

本文描述一种自制的多次曝光暗视野显微摄影的实验装置和使用方法，并提出了它的应用可能

This paper describes self-made multiexposure dark-field microphotographic experimental apparatus and operative methods , and its possible applications are also mentioned

3诊断新生儿先天梅毒缺乏金标准，应大力开展19S-IgM-FTA-ABS及暗视野显微镜检查；

There is no golden standard in the diagnosis of congenital syphilis . 19S IgM FTA ABS and dark field microscopy test should be taken ;

临床诊断可以通过在一种特殊显微镜下检查皮肤病灶样本（暗视野检查）得到确认。

The clinical diagnosis can be confirmed by examining a sample from a skin lesion under a special type of microscope ( darkfield examination ) .

在暗视野显微镜下观察到的血淋巴中的病毒粒子大小约为0.5μm；

Under a dark-field microscope virus particles were seen as numerous fine particles ( about 0 . 5 / μ m ) in the hemolymph .

最近，通过羊膜穿刺术获得孕妇的羊水，以其作暗视野显微镜观察，对先天性梅毒有诊断价值。

Recently , obtain the amniotic fluid of pregnant woman through amnion puncture art , make observation of dim view microscope with its , have diagnostic value to congenital syphilis .