共聚焦显微镜

共聚焦显微镜下LASIK术后角膜基质的变化

Corneal stromal changes under the confocal microscopy after LASIK

通过共聚焦显微镜观察或FCM分析，分别检测淋巴结切片、淋巳细胞中的标记DCs。

Labeled DCs were detected by confocal microscopy on LN sections or FCM analysis of LN cells . 3 .

采用激光共聚焦显微镜技术观察巨噬细胞内NF-κB的核移位情况；

The translocation of NF-kB of macrophages was observed by using laser scanning confocal microscopy ( LSCM ) .

流式细胞术联合共聚焦显微镜对G2和M期作用位点的鉴别意义

Discrimination of Anticancer Agent Action Loci at G_2 and M phases by Flow Cytometry and Confocal Microscopic Imaging

激光共聚焦显微镜分析细胞色素c（CytochromeC，CYTC）的释放。

Cytochrome c ( Cyt c ) release was assessed by confocal laser scanning microscopy .

本文运用激光扫描共聚焦显微镜和荧光探针技术，检测分裂细胞内DNA、RNA分布和含量的变化。

Morphology of the cell division and its content of DNA and RNA were measured under the laser scanning confocal microscope .

激光共聚焦显微镜和流式细胞仪分析显示LPS能与内皮细胞结合。

Analysis by laser confocal microscopy and flow cytometry revealed that LPS could bind to VEC .

3组细胞同时培养48h，用激光共聚焦显微镜观察3组细胞内钙离子的荧光强度。

The fluorescence intensity was detected with the laser confocal microscope .

而在对侧肢PCR和共聚焦显微镜的结果均为阴性。实验结果表明，肌肉直接注射DNA可以在肌肉得到表达，将会为治疗肌肉损伤带来新的方法。

The study reveals that direct naked DNA injection to the muscle shows expression in it , which may bring new treatment for the muscular injury .

通过激光共聚焦显微镜观察了Cd、Cu、Zn污染后黑藻叶片自发荧光的变化；

Changes of auto-fluorescence in H. verticillata leaf under Cd , Cu and Zn stress were analyzed by Laser Scanning Confocal Microscope ( LSCM );

为了明确突变亚基的亚细胞定位，构建了αⅡb基因A2334CGFP融合蛋白表达质粒并用激光共聚焦显微镜确定GFP融合蛋白的细胞内定位。

A newly constructed αⅱ bA2334C GFP fusion protein expressing plasmid was used to determine its subcellular localization by laser confocal scanning microscopy .

神经细胞膜NMDA受体蛋白激光共聚焦显微镜亚细胞定位研究

Subcellular localization of NMDA receptor protein on neuron membrane by confocal laser scanning microscopy

同时B1作用HeLa细胞后，通过共聚焦显微镜可观察到细胞色素C从线粒体释放。

Furthermore , the release of cytochrome c from mitochondria was detected using confocal microscopy in HeLa cells treated with B1 .

结论在采集快速变化的荧光图像时，CCD荧光显微镜系统优于激光扫描共聚焦显微镜系统。

Conclusions CCD fluorescent microscope is superior to laser scanning confocal microscope in collecting the quickly changing fluores - cent image such as DCF .

采用上述肝癌细胞的细胞涂片或细胞爬片进行荧光免疫细胞化学实验，于荧光显微镜和共聚焦显微镜下观察上述4种EP受体蛋白的表达和定位分布。

The expression and intracellular localization of EP receptors were determined by RT-PCR and fluorescent immunocytochemistry assay respectively .

激光共聚焦显微镜观察Ki-67抗原表达量的变化；

We observed the change of Ki-67 antigen by laser scanning confocal microscope ( LSCM ) .

ROS水平分别采用激光共聚焦显微镜和FCM检测；

The level of reactive oxygen species ( ROS ) was detected with confocal laser scanning microscope and FCM .

激光共聚焦显微镜显示，感染重组PML病毒的膀胱肿瘤细胞胞核内有散在的斑点样的绿色荧光亮点。

Laser confocal microscopy showed speck dots fluorescence in the PML / UM-UC-2 nucleus .

经染色体加倍处理所获得的2n花粉粒，体积明显比正常的花粉粒大，并将激光扫描共聚焦显微镜用于大花粉粒的倍性鉴定。

The volume of large pollen obtained from the experiment is larger than the normal one .

上述处理后的细胞经间接免疫荧光染色标记后，用激光共聚焦显微镜观察其细胞膜EGF受体的聚簇现象。

EGF receptors on the cell membrane were ob - served under a laser scanning confocal microscope after indirect immunofluorescence staining .

LASEK与PRK术后角膜组织结构的活体激光共聚焦显微镜观察

Clinical application of in vivo confocal microscopy through focusing in LASEK and PRK

在建立单细胞凝胶电泳的方法时，对DNA彗星图像进行了共聚焦显微镜观察和三维重建，比较了几种不同荧光染料的染色效果。

Laser scanning confocal microscope ( LSCM ) was introduced to investigate the 3-D distribution of DNA within the comet and four fluorescent dyes were compared when the single cell gel eletrophoresis was set up .

借助药理学试验和激光扫描共聚焦显微镜技术，进一步对该间接效应过程中是否有NO和H2O2的参与进行了探讨。

Whether NO and H_2O_2 were concerned with the adjustment in indirect ways was further explored by pharmaceutical experiment and laser-scanning confocal microscopy .

经共聚焦显微镜观察发现，集聚蛋白在活化的淋巴细胞中以帽化结构形式存在，且和CD3分子共定位，抗集聚蛋白抗体可以明显减少这种帽化结构的形成。

In activated lymphocytes agrin was co-capping with CD3 molecules and this capping-like structure formation was interfered by antibody against agrin .

本文用共聚焦显微镜测试氮化硅薄膜悬梁悬起的高度，确定出最适合接触式射频MEMS开关中使用的氮化硅生长工艺条件。

We test the height of silicon nitride suspended beam from different depositing condition in laser-scanning microscope and decide the most proper depositing condition for RF MEMS switch .

在荧光共聚焦显微镜下通过单光子激发成像在细胞体内检测到荧光信号，并对其进行消化等实验检测方法证明在体内定位在DNA和核的部位，体内体外检测一致。

Fluorescence was detected under one-photon excitation by spectral confocal multiphoton microscopes and digestion test demonstrated that the GO based hybrid can stain in cell nucleus in vivo , which was consistent with the results in vitro .

流式细胞技术检验细胞结合和摄取荧光标记的低密度脂蛋白（LDL）的能力，激光共聚焦显微镜观察验证。

The binding and internalization of LDL to LDL receptor were detected by flow cytometer ( FCM ) and co - focus microscope .

采用间接免疫荧光细胞化学法和激光共聚焦显微镜进行连接蛋白Cx43定位的测定；

The localization of Cx43 proteins were performed by indirect immunofluorescence histochemical analysis and detected by confocal microscopy .

GFP和双链RNA进入C6/36蚊虫细胞，72h后收集细胞，用激光共聚焦显微镜和流式细胞仪检测GFP的表达差异，用半定量RTPCR检测GFP的mRNA的表达水平。

GFP protein expression was assessed using confocal laser microscope and flow cytometer , and the expression level of GFP mRNA was determined by relative quantitative RT-PCR .

结果在激光扫描共聚焦显微镜下，可以清晰地观察Cx43和细胞膜。

Results The distinct images of Cx43 and cell membrane of detrusor muscle could be obtained by the LSCM .