尘螨

目的：探讨尘螨特异性IgE和白细胞介素4（IL-4）在脱敏治疗中的作用。

Objective : Survey the house dust mite specific IgE and interleukin-4 's ( IL-4 ) role in the desensitization therapy .

目的构建并克隆屋尘螨抗原Derp2基因，在大肠杆菌中初步表达。

AIM To construct and clone the gene of the house dust mite antigen Der p2 , which is expressed in E.

地毯可以成为滋生细菌和尘螨的温床，这会导致呼吸问题、湿疹和其他过敏症状等健康问题。

Carpets can be a breeding ground for bacteria and dust mites7 , which can lead to health issues including breathing problems , eczema and other allergy8 symptoms .

卫生专家丽莎·阿克利博士说：“这项研究很重要，因为未经清洗的地毯可能成为细菌和致病菌的家园，尤其是尘螨，这可能使一些人更容易患尘螨排泄物过敏引起的哮喘、湿疹和常年过敏性鼻炎。”

Hygiene9 expert Dr Lisa Ackerley said : ' This research is of concern as unwashed carpets can become homes to bacteria and germs and in particular dust mites , which can put some people at greater risk of asthma10 , eczema and perennial11 allergic12 rhinitis caused by allergies13 to dust mite6 faecal matter .

结果：①121例哮喘患儿尘螨过敏原皮肤点刺试验总阳性率为28.10%，高于对照组（P＜0.05）。

Result : ① The total positive rate of skin prink test of 121 children with asthma was 28.10 % .

结论：屋尘螨、粉尘螨是广州地区小儿荨麻疹和AD患者最常见的变应原。

Conclusion : Mites were common allergen in infants and children with urticaria and AD.

测定人血清中抗蒿属花粉和尘螨特异性IgGELISA间接法的建立

Detection of Human Specific IgG to P or M with ELISA

利用ELISA技术检测尘螨变应原的生物活性

To Detect the Biological Activity of Mite Allergen Product Using the ELISA Assay

利用套式PCR技术鉴别屋尘螨和粉尘螨主要变应原基因及其在尘螨疫苗研制中的应用

To distinguish allergen genes of Dermatophagoides pteronyssinus from Dermatophagoides farinae by nested PCR and its application for the vaccine manufacture

间接ELISA法对常年性变应性鼻炎患者血清中屋尘螨变应原特异性IgE的分析

Analysis of Dermatophagoides Pteronyssinus-specific IgE Concentration in Sera of Perennial Allergic Rhinitis Using Indirect ELISA

抗粉尘螨主要变应原Derf1单克隆抗体的制备与鉴定

Preparation and Identification of Monoclonal Antibodies Against Der f1 Allergen

屋尘螨抗原Derp2基因的克隆和表达

Cloning and expression of gene of house dust mite antigen Der p2

目的探讨尘螨过敏性哮喘患者血清免疫球蛋白IgE、IgG亚类的临床意义。

Objective To explore the clinical significance of serum immunoglobulin in asthma patients allergic to house dust mites .

摘要目的用聚合酶链反应（PCR）技术对粉尘螨和屋尘螨进行鉴别和检测。

Abstract Object To identify and detect Dermatophagoides farina ( D.f ) and Dermatophagoides pteronyssinu ( D.p ) by Polymerase Chain Reaction ( PCR ) technology .

户尘螨SPT和特异性IgE结果符合率为97%。

The consistence rate of dermatophagoids pteronyssinus SPT and specific IgE was 97 % .

空调空气滤网灰尘中尘螨变应原Derf1和Derp1基因的检测

Detection of the genes encoding house mite allergens Der f1 and Der p1 from the air-filters of air-conditioners

目的应用PCR技术检测空调空气滤网灰尘中尘螨变应原Derf1和Derp1的基因。

The genes encoding human mite allergens Der f1 and Der p1 could be detected from the air-filters of air conditioners .

吸入型哮喘患者尘螨皮肤挑刺试验、鼻腔激发试验和特异性IgE的测定

Skin prick test , nasal provocation test and measurement of specific IgE with dust mite extract in allergic asthmatic patients

FasL基因转染树突状细胞对屋尘螨致敏/激发小鼠气道炎症影响的研究

Effects of FasL gene-modified dendritic cell on the airway inflammation in mice sensitized / challenged by house dust mite allergen

比较后结论为使用Cocas液提取的尘螨蛋白质浓度低，蛋白点少。

There were low protein concentration and less protein spots with Coca 's extract .

方法用随机引物PCR对粉尘螨和屋尘螨的DNA进行扩增，选择三个扩增条带进行测序和分析。

Methods A randomly primed PCR ( RP-PCR ) technology were used to amplify the DNA of D , f and D.p , three amplified bands were selected for DNA sequencing and analysis .

结论尘螨是AD的主要变应原，卡介菌多糖核酸联合曲安奈德益康唑乳膏治疗AD的疗效优于单用曲安奈德益康唑乳膏。

Conclusions Dust mites are the most common allergens in AD. Combined BCG-PSN and triamcinolone acetonide and econazole nitrate cream therapy is superior to triamcinolone acetonide and econazole nitrate cream only .

屋尘螨重组变应原Derp2的表达、纯化及免疫学活性鉴定

Expression , purification and identification of the recombinant allergen Der p2 from Dermatophagoides pteronyssinus and investigation on its immunological activities

检测不同地区尘螨主要变应原的序列多样性，有助于开发更有针对性的疫苗组分，以及特异性保护性IgG的筛选。

To examine the sequence polymorphism of major allergens of dust mites is beneficial to the development of more pertinent vaccines and the screening for protective IgG .

方法以CD63/CD203c/CD45抗体组合，建立用FCM分析户尘螨致敏的嗜碱性粒细胞脱颗粒的方法。

Methods The protocol for FCM analysis of basophil degranulation in allergy to dust mite by CD63 / CD203c / CD45 combination was established .

结果两种尘螨过敏原浸液风团及伪足的直径有显著性差异（p<0.05），Alutard-SQ皮肤试验结果与特异性IgE水平相关性好，国内过敏原浸液皮肤试验与特异性IgE水平相关性差。

There was also statistical significance difference between the correlation of the serum specific IgE levels and size of SPT wheal by both allergens .

结论：用屋尘螨变应原在肠道菌群失调小鼠模型上气道激发，诱导小鼠产生Th2细胞优势的变应性气道反应。

Conclusions : An allergic airway response model was established successfully by using dermatophagoides pteronyssinus allergens to induce mice with intestinal microflora disruption .

本课题组前期研究已经证实VEGF在职业哮喘诱发因素甲苯二异氰酸盐（TDI），屋尘螨以及过氧化氢等能够导致HBE通透性增高的过程中发挥重要作用。

Our previous studies have proved that VEGF plays an important role in occupational toluene diisocyanate ( TDI ) induced permeability of AEC .

FasL和屋尘螨基因共转染树突状细胞诱导屋尘螨致敏/激发小鼠免疫耐受的实验研究

Induction of Allergen - specific Immune Tolerance by Dendritic Cell Co-transfected with FasL and Der p ⅱ Genes in Mice Sensitized / challenged by HDM Allergen

目的：构建能够携带外源蛋白屋尘螨抗原（Derp2）基因并能在胞壁表达的E.coliBCG穿梭载体。

AIM : To construct the E. coli BCG shuttle vector carrying and expressing dust mite antigen gene Der p2 on cell wall of mycobacterium vaccae .