基因表达载体

基于RNA复制子GHRH基因表达载体的构建及在293细胞中的表达

The Construction of GHRH Gene Expression Vector Based on RNA Replicon and Its Expression in 293 Cell

人钙调素基因表达载体的构建及在大肠杆菌DH5α中的表达

Construction of human calmodulin gene expression vector and expression of calmodulin protein in E.coli DH5 α

肿瘤靶向性TRAIL基因表达载体抗乳腺癌的体外研究

Experimental Study on Anti-breast Cancer Activity by the Tumor-targeted TRAIL-expressing Vector in Vitro

可溶性人TRAIL基因表达载体的构建及其诱导肿瘤细胞凋亡的实验研究

Construction of human soluble TRAIL expression vector and its effect on apoptosis of tumor cells

鹿腐蹄病A型节瘤拟杆菌纤毛蛋白与绵羊IL－2融合基因表达载体的构建与表达

Construction of a Plasmid Expressing a Fusion Construct of D.nodosus Serotype A Pili Gene and Ovine IL-2 Gene

pBⅠ121载体酶切位点添加及拟南芥Na~+/H~+逆向转运蛋白基因表达载体的构建

Modification of pB ⅰ 121 Vector and Expression Vector Construction Na ~ + / H ~ + Antiporter of Arabidopsis thaliana

目的：构建膜结合型人肿瘤坏死因子与谷胱甘肽S转移酶（GST）的融合基因表达载体。

Objective : To construct a GST ( Glutathione-s-transferase ) - TNF fusion gene .

抗猪瘟病毒Ribozyme基因表达载体的构建

Construction and Sequencing of Vectors Expressing a Ribozyme Gene Against Hog Cholera Virus

抗菌肽cecropinP1基因表达载体构建与表达

Construction and expression of Porcine Cecropin P1 expression vector

肝癌特异性超抗原SEA（D227A）基因表达载体的构建与表达

The construction and expression of a hepatocellular carcinoma-specific superantigen SEA ( D227A ) expression vector

1α，25二羟维生素D3靶控ERα基因表达载体构建及其联合他莫西芬对ER阴性乳腺癌细胞抑制效应的研究

The Construction of ER α Expression Vector Containing Vitamin D _3 Response Elements , and Its Effects on ER-negative Breast Cancer Cells in Combination with Tamoxifen

小麦WVp-1基因表达载体的Gateway技术构建及其遗传转化

Construction and Transformation of the Expression Vector of Wheat WVp-1 Gene Using Gateway Technology

目的分别构建三种启动子调控的大肠杆菌嘌呤核苷磷酸化酶（PNP）基因表达载体，检测、分析三者的表达差异。

Objective To construct three expression vectors harboring PNP ( purine nucleoside phosphorylase ) gene under three different promoters .

CMV-Pf-CP基因表达载体的构建及转化西番莲（Passifloraedulis）的研究

Construction of expression vector of CMV-Pf-CP gene and transformation of Passiflora edulis

禽脑脊髓炎病毒VP1、VP3、VP0基因表达载体的构建

Construction of Eukaryotic Expression Recombinant Plasmids of VP Gene of Avian Encephalomyelitis Virus

目的构建人胰腺癌PTCH基因表达载体并诱导融合蛋白表达。

Objective To clone human pancreatic cancer gene PTCH and construct pET22b / PTCH expression plasmid .

HSV-tk自杀基因表达载体的构建及HSV-tk/GCV系统对肝癌细胞作用的体外研究

Construction of Expression Vector Containing HSV-tk Gene and in Vitro Study on Killing Effects of HSV-tk / GCV System on Human Hepatocellular Carcinoma Cell Line SMMC-7721

结论已成功构建NDV强毒株F蛋白裂解位点串联基因表达载体，为建立NDV强弱毒株的ELISA鉴别方法提供理论依据。

Conclusion A prokaryotic expression vector for F protein schizolysis site gene cascade of virulent NDV strain was successfully constructed , which provided a theoretical basis for differentiation of virulent and low virulent NDV strains by ELISA .

结论人源性丙肝病毒抗体轻链基因表达载体是高效的，为下一步构建丙肝病毒抗体Fab段基因重组载体奠定了基础。

Conclusion The expression vector of humanized antibody light chain gene of against HCV is constructed effectively which laya solid foundation for further constructing the recombinant expression vector of humanized antibody Fab fragment against HCV .

方法：用电穿孔法将MCT1反义基因表达载体转染进人肺腺癌A549细胞中，以抑制MCT1基因表达。

Methods : MCTl antisense gene expression vector was introduced into A549 cells with electroporation to inhibit the expression of MCTl gene .

目的分别构建巨细胞病毒（cytomegalovirus，CMV）通用启动子和甲胎蛋白（αfetoprotein，AFP）组织特异性启动子调控的PNP基因表达载体并分析二者表达的差异性。

Objective To construct two expression vectors harboring PNP gene under the control of cytomegalovirus ( CMV ) promoter and the α - fetoprotein ( AFP ) tissue-specific promoter and detect their expression in different cell lines .

目的为提高体外转基因（exvivo）治疗帕金森病大鼠模型的疗效，本实验拟比较两种基因表达载体介导的外源基因在两种不同运载细胞的瞬时表达效率。

Objective Two types of cells transfered by two different types of vectors containing gene of interest were used to compare their expression efficiency in vitro , in order to provide an effective protocol for ex vivo gene therapy in a rat model of Parkinson disease .

本实验猪防御素pBD-1基因表达载体的成功构建，为进一步研究pBD-1基因表达产物的抗菌活性及其抗菌机理打下了基础。

The successful construction of pBD-1 gene expression vector laid down the foundation in research of antibacterial activities , and antibacterial mechanism .

重组人晶状体蛋白CRYAB基因表达载体的构建为研究CRYAB基因的功能及进一步研究先天性白内障的发病机制奠定了基础。

It is concluded that expression recombinant of α B-crystallin CRYAB gene will be helpful in studying function of CRYAB gene .

结论三种PNP基因表达载体的成功构建为利用PNP/MeP-dR系统进行肝癌的靶向性基因治疗打下坚实的基础。

The target PNP gene expressions were detected both in AFP positive and in negative hepatocellular carcinoma cell lines . Conclusion The successful constructions of three PNP gene vectors will lay a substantial foundation for the target gene therapy of hepatocellular carcinoma with the PNP / Mep-dR system .

本研究在探索银耳遗传转化体系的基础上，构建了高赖氨酸蛋白基因表达载体，利用限制性内切酶介导整合法（REMI），首次把高赖氨酸蛋白基因导入银耳芽孢，获得了转基因菌株。

In the study , on the basis of studying the system of genetic transformation of Tremella fuciformis , a lysine-rich protein gene expression vector was constructed , and then transferred into Tremella fuciformis spores with Restriction Enzyme-Mediated DNA Integration ( REMI ) .

β淀粉样蛋白基因表达载体的构建及转化

Construction and Transformation of Expression Vectors Including Amyloid β Peptide Gene

丙型肝炎病毒截短型核心基因表达载体的构建与表达

Construction and expression of prokaryotic expression vector for truncated HCV-core gene

人甲状旁腺激素基因表达载体的构建

The construction of the plasmid vector of human parathyroid hormone gene

抗线虫基因表达载体构建与转化甘蔗研究初报

Construction of expression vector of nematode-resistant gene and transformation of sugarcane