鸟枪法

目前已有多种对串联质谱与数据库中多肽的理论质谱的一致性进行评估的高通量计量算法用于鸟枪法蛋白质组学（shotgunproteomics）研究。

High throughput scoring algorithms that are used to find the match of a tandem mass spectrum to a predicted mass spectrum of a peptide within a database have been applied in shotgun proteomics .

论文第一部分利用鸟枪法测定了hb1f（nr5a2）的全基因组序列。

The first chapter of this dissertation elaborated the process of obtaining the whole genomic sequence of hb1f ( nr5a2 ) gene via shotgun approach .

本课题采用全基因组鸟枪法（Whole-Genome-Shotgun）对保加利亚乳酸杆菌2038株进行全基因组测序。

Bulgaricus and ultimately would help us improve this bacterium . In our project , we use a " whole-genome-shotgun " method to sequence Lactobacillus delbrueckii ssp .

日本血吸虫全基因组鸟枪法序列拼接系统的设计与实现

Design and Implementation of Whole Genome Shotgun Assembly System for Japan Schistosome

鸟枪法蛋白质鉴定质量控制方法研究进展

The Progress of Protein Quality Control Methods in Shotgun Proteomics

首先，本文简要介绍了鸟枪法测序和序列拼接的基本流程和研究现状。

First , the basic processes of shotgun sequencing and genome assembly are introduced .

采用鸟枪法克隆到了枯草芽孢杆菌α淀粉酶基因。

The α amylase gene of Bacillus subtilis HN 503 was successfully cloned by shotgun method .

微生物全基因组鸟枪法测序

Whole Microbial Genome Shotgun Sequencing

串联质谱蛋白质鉴定方法的研究基于离子阱串联质谱仪的鸟枪法是一种高通量的蛋白质鉴定方法。

Shotgun method based on ion trap tandem mass spectrometry is a high-thought protein identification strategy in proteomic research .

但是对于包含大量重复序列的大型真核生物，全基因组鸟枪法序列拼接的实际应用仍然充满挑战性。

But there are still many challenges in the practice whole genome shotgun assembly for large eukaryote genomes which contains lots of repeat sequences .

介绍了鸟枪法蛋白质组的系统组成，研究进展以及在医药领域的应用情况。

This review introduces the components of this proteomics strategy and describes selected recent applications and emerging innovations in the evolution of shotgun proteomics .

本文介绍一种基于基因组扫描（鸟枪法）和生物信息学相结合的寻找微生物新化合物的平台。

A new platform based on the genome scanning technology ( shot gun ) and bioinformatics to discover novel compounds from microbial origin was introduced .

目的：通过应用鸟枪法蛋白质组学技术发现早期诊断和逆转前列腺癌转移的分子标志。

OBJECTIVE : To explore the mechanism of prostate cancer metastasis and search for the potential markers for early diagnosing and reversing prostate cancer metastasis by shotgun-MS.

评价诱饵库设计，搜索策略，匹配误差和质量控制对鸟枪法蛋白质组学中肽段鉴定精确性的影响。

Evaluation of the effect of decoy design , search strategy , mass tolerance and quality control method on the accuracy of peptide identifications in shotgun proteomics .

鸟枪法蛋白质组学技术是另一条研究蛋白质组的技术路线，随着多维色谱技术和质谱技术的发展，用鸟枪法技术路线来鉴定生物体蛋白质组也得到了迅速的发展。

Shotgun strategy is taken as another method for proteome study . With the development of multi-dimensional chromatography and mass spectrometry , the application of shotgun proteomics is progressing .

以鸟枪法为代表的新兴蛋白质组学技术在生命科学研究中的广泛应用，使得人们从整体水平研究蛋白质组成和调控活动规律成为可能。

The shotgun method , as the representative of emerging proteomic technologies , has been widely used in life science research , which can be expected to show the changes in protein expression profile .

全基因组测序主要有二种策略，一种是分级鸟枪法测序，另一种是全基因组鸟枪法测序。

Two strategies introduced for whole genome sequencing , one is clone by clone method , the other is whole genome shotgun sequencing , for microbes which are very important to us , whole genome shotgun sequencing method is very convenient .