噬菌体载体

用λ噬菌体载体构建了条斑紫菜Porphyrayezoensis丝状体cDNA表达文库，并对文库进行了随机测序，对随机测序形成的719条表达序列标签（EST）序列进行了初步分析。

A laver Porphyra yezoensis sporophyte cDNA expression library has been constructed with λ phage vector and was randomly sequenced .

本实验构建了EGFR为靶向的重组T7噬菌体载体疫苗，并进一步探讨在体的免疫活性以及抗肿瘤作用，为抗肿瘤疫苗的发展奠定基础。

Therefore , the recombinant bacteriophage T7 vaccine was constructed targeting human EGFR . The immune reactivity of anti-tumor was studied in our experiment both in vitro and vivo , for providing a basis of study on anti-tumor vaccine .

通过噬菌体载体筛选胶质瘤细胞结合的内化短肽

Selection of targeted glioblastoma tumor cell-binding and internalizing peptides through phage display vector

在核酸杂交中设计了噬菌体载体克隆位点互补探针，以更加准确地排除无外源基因插入的克隆；

Additionally we used the probe oligodeoxynucleotide complementary to vector clone site sequences to identify clones which were not inserted exogeneous genes .

然后利用限制性内切酶SfiⅠ、NotⅠ分别双酶切抗体基因和噬菌体展示载体。

Then the antibody genes and the phage display vector were digested by restriction endonucleases Sfi ⅰ and Not ⅰ .

噬菌体展示载体的构建

Construction of Vector for Phage Display

以poly（A）~+mRNA为模板，噬菌体λgt10DNA为载体构建了黑曲霉3758的cDNA的文库。

A cDNA library was constructed using poly ( A ) ~ + mRNA of Aspergillus niger 3758 as template and phage Agt 10 DNA as vector .