噬菌体文库

在筛选cDNA文库时则增加了一个将噬菌体文库转化成质粒文库的步骤。

In screening of cDNA library , conversion of λ phage library to plasmid library was carried out before screening .

非免疫性人抗体Fab片段噬菌体文库的构建

Construction of a Non-immunized Human Fab Fragment Phage Library

利用PCR筛选噬菌体文库人免疫球蛋白重链Cγ基因的一种新方法

A new method used to screen the human immunoglobulin heavy chain C γ gene by means of PCR-screening phage library

HIV-1p24单链抗体的构建及其噬菌体文库筛选

Construction and selection from a peptide phage display library of the single-chain Anti-HIV-1 P24

总之，通过对抗猪脂肪细胞膜蛋白单链抗体噬菌体文库的构建，我们对抗猪脂肪细胞膜蛋白单链抗体的研究进行了一定的摸索，这将为该抗体的进一步研究打下基础。

In a word , we take some method in researching single chain antibody by constructing scFv phage library against porcine adipocyte plasma membrane protein , which is very important to study immunization against adipocyte plasma membranes .

人大肠癌抗原基因cDNA噬菌体表达文库的构建和鉴定

Construction and identification of cDNA library for colorectal carcinoma antigens

目的：构建人源性大肠癌抗原cDNA噬菌体表达文库。

AIM : To construct a colorectal carcinoma cDNA phage expression library .

经体外包装，建立cDNA的噬菌体表达文库。

After packaging , the OB cDNA library was constructed .

重组Ig结合分子噬菌体展示文库的构建及选择研究

Construction and Selection of Phage Displayed Library of Recombinant Ig-binding Molecules

人工进化丙型肝炎病毒（HCV）C和E1区噬菌体展示文库的构建及初步筛选

The Construction and Primary Screening of Phage Display Library of Hepatitis C Virus C and E1 Evolved with Artificial Pattern

抗五步蛇毒ScFv噬菌体显示文库的构建及表达

The construction and expression of anti-agkistrodon acutus guenther ScFv phage displaying library

人源性抗HBsAg抗体Fab段噬菌体呈现文库的构建、筛选及阳性克隆核苷酸序列测定

Screening of phage antibodies against HBsAg from the constructed phage display library and sequencing of the positive clones

微小隐孢子虫T7噬菌体展示文库的构建

Construction of T7 phage display library from Cryptosporidium parvum

结果构建成含2.39×106pfu/ml重组子的人大肠癌cDNA噬菌体表达文库，重组率为97.5%。

Results The human colorectal carcinoma cDNA phage library consisting of 2.39 × 10 6 pfu / ml bacteriophages was constructed , and the recombinant percentage was 97.5 % .

克隆技术如cDNA的噬菌体表达文库技术的发展加速了编码变应原的候选cDNAs的分离。

The development of phage surface display technologies for cloning cDNA has speeded up the isolation of candidate cDNAs encoding allergens .

为了进一步研究Era的功能，本课题组陈南春高级实验师用地高辛（DIG）标记的Era蛋白从大肠杆菌基因组噬菌体表达文库中筛选了Era相互作用分子。

In order to elucidate the function of Era , Chen NC of our group screened the Escherichia coli Era-associated protein from phage expression library of Escherichia coli genomic DNA using Dig-labeled Era protein .

结论成功构建了微小隐孢子虫T7噬菌体展示文库。

These results suggests that the T7 phage display library from C. parvum is constructed successfully .

结论成功构建高质量人源性大肠癌cDNA噬菌体表达文库，适合于免疫筛选cDNA克隆的人大肠癌相关抗原基因。

Conclusion The successful construction of the human colorectal carcinoma cDNA phage library may have an important role in the searching for human colorectal carcinoma associated antigen genes through immunoscreening cDNA clones .

对虾血细胞T7噬菌体展示文库构建及功能基因淘选

Construction of T_7 Phage Display Library from Chinese Shrimp 's hemocytes and Panning of Functional Genes from the Library

通过T7噬菌体展示文库筛选免疫相关基因在安氏隐孢子虫上的应用未见报道。

There is no reported that T7 phage display library screening is used for screening immune-related genes in C. andersoni .

以细菌刺激后提取的对虾血细胞mRNA为材料，构建中国对虾血细胞的T7噬菌体展示文库，并且进行文库扩增，扩增后文库的滴度达到10~（11）pfu／ml。

T_7 Phage Display Library was constructed from challenged shrimp 's hemocyte mRNA , and then the library was amplified to reach the titer of 10 ~ ( 11 ) pfu / ml.

目的：构建HIV-1GagPR蛋白P2/NC切割位点序列随机化的噬菌体展示文库，研究HIV-1蛋白酶（proteasePR）耐药性突变对其敏感切割序列的影响。

Objective : To construct the phage display library of randomized P2 / NC protease cleavage sequences in HIV-1 Gag protein , so as to provide evidences for studying the effect of drug resistance-associated mutations on susceptible cleavage sequences of HIV-1 PR.

从噬菌体多肽文库中筛选α-葡萄糖苷酶的抑制剂

Screening An α - glucosidase Inhibitor from A Phage-displayed Peptide Library

目的通过构建汉滩病毒M片段的噬菌体展示文库，筛选汉滩病毒包膜蛋白的抗原表位。

Objective To study the epitope of Hantaan virus M fragment by phage display .

结论所构建的噬菌体展示文库的库容和多样性符合筛选的要求。

Conclusion The capacity and diversity of the constructed library are enough for screening .

采用4轮连续筛选富集抗除虫菊酯的噬菌体抗体文库。

The enrichment of scFv antibody library was performed by four rounds of successive screening .

噬菌体展示文库的筛选技术

The Selection Techniques of Phage Display Libraries

结果制备了库容为8×107pfu/ml的噬菌体展示文库。

Results A recombinant phage display library with total of 8 × 10 7 pfu / ml was established .

构建鼠源性抗日本血吸虫噬菌体抗体文库，以探索制备抗日本血吸虫单克隆抗体的新方法。

The murine phage antibody library against Schistosoma japonicum was constructed in order to develop a new way preparing monoclone antibody against S.japonicum .

方法用文库构建试剂盒，以健康人外周血淋巴细胞为材料，建立噬菌体抗体文库，并对其进行鉴定。

Methods Taking human 's blood cells as raw material , the phage antibody library was constructed with Kits of Recombinant Phage Antibody System and testified .