噬菌体展示技术

应用噬菌体展示技术，从猪脂肪细胞免疫的小鼠脾细胞mRNA中构建出单链抗体（scFv）cDNA文库。

Using phage display technology , cDNA library of anti - adipocyte membrane scFv was constructed from mRNA of mice spleen cells , which had been immunized by pig adipocytes .

通过噬菌体展示技术从含高滴度抗乙肝表面抗原（HBsAg）抗体的人外周血淋巴细胞获得抗HBsAgFab抗体的轻、重链基因。

The genes of anti-HBsAg Fab antibody were obtained from human peripheral blood lymphocytes of volunteer with high antibody titer to HBsAg by using of phage display technology .

采用噬菌体展示技术筛选黄曲霉毒素B1模拟抗原表位

Mimic epitope of aflatoxin B_1 screened by phage display technique

噬菌体展示技术筛选干扰素α启动子DNA结合蛋白

Screening of binding proteins to interferon-alpha promoter DNA by phage display technique

噬菌体展示技术筛选诱导型NOS基因启动子DNA结合蛋白的研究

Screening of promoter DNA-binding protein of iNOS gene by phage display technique from human liver cDNA library

利用噬菌体展示技术对突变库进行4轮富集筛淘及ELISA鉴定；以ELISA方法测定所获阳性克隆的特异性。

Hepatoma cell-specific scFvs were selected after four rounds of panning by phage display and ELISA detection .

应用噬菌体展示技术能成功地区分糖尿病相关性IAA与IA的独特型。

Phage display can distinguish idiotypes of diabetes-related IAA from IA .

噬菌体展示技术筛选bFGF抗原表位

Screening antigen epitope of bFGF by phage display

人宫颈癌Hela细胞穿透肽的噬菌体展示技术筛选

Screening of display technology for phages targeting human cervical carcinoma Hela cells-penetrating peptides

噬菌体展示技术筛选DNA错配修复蛋白MutS高亲和性抗体

High affinity antibody on DNA mismatch repair protein MutS through method of phage display

用T7噬菌体展示技术筛选HCV核心蛋白的相互作用蛋白

Screening of proteins interacting with hepatitis C virus core protein from T7-phage display library

应用噬菌体展示技术进行猪瘟病毒结构（糖）蛋白E2和E～（rns）中和抗原表位鉴定和比较

Identification and Comparison of Neutralizing Epitopes of Structural Glycoprotein E2 E ~( rns ) of Classical Swine Fever Virus Using Phage Display

应用展示技术筛选不同功能的核酸、肽和蛋白是近年的研究热点，目前应用的展示技术包括噬菌体展示技术、核糖体展示技术及mRNA体外展示技术等。

Using display technologies to isolate nucleic acids , peptides and proteins has been studied intensively in recent years . The display technologies now applied include phage display , ribosome display , and in vitro mRNA display .

结论利用噬菌体展示技术筛选到了黄曲霉毒素B1模拟抗原表位，并以其代替黄曲霉毒素B1偶联抗原建立了免疫学检测方法。

Conclusion The mimic epitope of aflatoxin B_1 antigen was obtained successfully by the phage display technigue and it is effective substitutes for aflatoxin B_1-protein conjugate in the immunoassay .

目的：通过噬菌体展示技术筛选得到与FGFR结合的bFGF模拟短肽，为bFGF肽类抑制剂的研发提供实验基础。

Objective : To obtain the bFGF mimic peptide binding to FGFR via phage display , and to provide the base for developing peptide agonist of bFGF .

通过噬菌体展示技术淘选抗细菌HrpA蛋白的单链抗体。

In this paper , phage display technology was used to screen scFv against HrpA .

应用噬菌体展示技术制备抗Met（HGF受体，一个与肿瘤发生、侵袭和转移相关原癌基因产物）特异性、高亲和力的全人Fab片段。

High affinity , fully human antibody fragment Fab that specifically bind to HGF receptor , Met ( a proto-oncogene product and a key molecule on tumorigenesis , invasion and metastasis ), was selected from phage display antibody library .

利用噬菌体展示技术，对荷人结肠癌CL-187裸鼠进行体内筛选，获得在肿瘤组织中富集的一条十二肽SVSVGMLPSHAP。

A dodeca-peptide SVSVGMLPSHAP that targets human CL-187 tumor was identified in vivo by phage display technology .

目的探讨噬菌体展示技术在鼠CD137配体（CD137L）活性研究中的应用。

Objective : To explore the application of phage display technique in study of rat CD137L activity .

但本实验的设计是对获得各种新型高特异性高亲和力Ig结合分子的一次科学的探索，同时也为噬菌体展示技术在免疫球蛋白结合分子进化研究中的应用提供了新的可借鉴方法。

However , the design of experiment is a scientific exploration to obtain various of new Ig-binding molecules with high specificity and activity . The study provides a new path in application of phage-display technology realizing molecular evolution of proteins .

本课题拟以LPS为刺激物构建大鼠炎症模型，利用噬菌体展示技术体内筛选方法，以获得高效抑制趋化因子趋化作用的小肽，为抗炎药物的研发提供先导肽。

In this study , an inflammation model stimulated by LPS in rats was established , a new small peptide with efficient inhibition of chemotaxis was developed by using in vivo phage display screening method , it may be as a leading peptide in the research of anti-inflammatory drug .

方法以人脑胶质瘤细胞系SHG-44免疫小鼠，取其脾淋巴细胞，应用噬菌体展示技术构建噬菌体抗体库，并筛选抗人脑胶质瘤单抗。

Methods Six Balb / c mice were immunized with intact SHG 44 . The phage antibody library was generated from spleen lymphocytes by means of phage display , followed by preliminary screening to acquire McAb against glioma .

噬菌体展示技术用于寄生虫病的研究

The application of phage display techniques in the studies of parasitic diseases

利用噬菌体展示技术筛选胰岛素模拟肽

Identification of Insulin Mimetics from a Phage - displayed Library

本文回顾了噬菌体展示技术的研究进展。

The progress in phage display techniques is reviewed in this article .

噬菌体展示技术筛选抗轮状病毒多肽的试验研究

Screening for Peptides of Anti-rotavirus by Phage - displayed Technique

噬菌体展示技术已被广泛地应用于生物学研究的各个方面。

Recently , phage display techniques have been applied to various biological aspects .

噬菌体展示技术在蛋白质研究中的应用

Application of phage display technology in protein researches

噬菌体展示技术及其在感染性疾病中的应用

Phage Display and Its Application to Infectious Diseases

结果提示噬菌体展示技术是制备抗体的一种有效的新途径。

Results suggest that phage display technique be a new way of making antibodies .