融合蛋白

目的：研究转位肽/粒酶B融合蛋白对细胞生长的抑制作用。

AIM : To investigate the inhibitory effect of translocating peptide / granzyme B fusion protein on cell growth .

结论融合蛋白构建成功，可用于HIV的快速检测。

Conclusions The fusion protein has the potential in rapid detection of HIV .

westernblot检测纯化融合蛋白的免疫反应性。

The immunogenicity of recombinant antigen purified was tested with Western blot .

通过westernblot及ELISA法，检测融合蛋白的表达。

The expression of the fusion protein was detected by Western blot and ELISA .

可分泌性胰高血糖素样肽1融合蛋白cDNA克隆的构建

Construction of cDNA clone of the secretory glucagon-like peptide-1 fusion protein

WESTERNBLOTTING印迹表明纯化的融合蛋白具有特异的免疫反应特性。

Western blotting also demonstrated the immune specificity of the purified fusion protein .

经WESTERNBLOTTING免疫印记分析，该融合蛋白具有较好的反应原性。

Western blotting analysis suggests a good reactogenicity involving of the fusion protein .

以从PAGE凝胶上切下的融合蛋白免疫BALB/c小鼠制备多克隆抗血清。

Coli . The fusion protein from PAGE gel was used to immunize BALB / c mice and prepare polyclonal antiserum .

新城疫病毒中国株F（48）E9融合蛋白基因序列分析

Sequence Analysis of Fusion Protein Gene of the Chinese Strain F_ ( 48 ) E_9 of Newcastle Disease Virus

方法：猪囊尾蚴cDNA表达文库中筛选出的β－半乳糖苷糖－猪囊虫cDNA编码的融合蛋白为抗原，进行简化的westernblot分析。

METHODS : Fusion protein of β galactosidase Cysticercus cellulosae cDNA was analysed with simplified Western blot .

应用m2-Gi1α融合蛋白鉴别M2受体的特异性药物

Identification of some specific drugs for M2 receptor using m2-Gil α fusion protein

SDS-PAGE检测到40kD大小的融合蛋白。

A 40 kD fusion protein was detected by SDS-PAGE .

重组融合蛋白Fab/IFNαA体外抗HBV作用的实验研究

Studies of the effect of a fusion protein Fab / IFN α A on hepatitis B virus in vitro

FAS抗原胞外区片段GST融合蛋白的产生

Production of recombinant FAS antigen and GST fusion protein

目的构建人干扰素α1和抗菌肽B的融合蛋白表达质粒pHC，并初步研究融合蛋白的抗病毒活性。

AIM To construct a plasmid expressing fusion protein , and to study the antiviral activity of the proteins .

三氧化二砷对转染表达两种早幼粒细胞性白血病融合蛋白的K562细胞的影响

Effects of arsenic trioxide on K562 cells stably expressing two promyelocytic leukemia-specific fusion proteins

目的：对乙型肝炎病毒重组多表位抗原基因在大肠杆菌中表达的融合蛋白P/BPT进行免疫原性分析，以期为HBV预防/治疗性疫苗的研制奠定实验基础。

Objective : To express protein of the therapeutic multi-epitope gene of Hepatitis B virus and to study its immunogenicity .

经Amyloseresin纯化后，MBP/Ag85B融合蛋白的纯度可达95%以上。

The purity of the MBP / Ag85B fusion protein was over 95 % after purification with Amylose resin .

OsPTF1与GFP融合蛋白定位于细胞核中。

A OsPTFl : GFP fusion protein was localized in the nucleus .

人内皮抑素与IL3信号肽融合蛋白真核表达载体的构建

Construction of the eukaryotic expression vector expressing the fusion protein of human endostatin protein and IL_3 signal peptide

采用分子生物学专业分析软件vectorNTI对融合蛋白分子进行分子特性分析和预测，并根据其分子特点设计纯化方案。

The characteristic of fusion protein was analyzed and predicted using software Vector NTI .

纯化的融合蛋白和纯化的核蛋白均为SDSpage单点纯，并具有良好的抗原活性。

The purified fusion protein and nuclear protein were single band by SDS PAGE . Both of them had available antigen activity .

westernblot检测mAb对变性Ig融合蛋白的反应性。

Western blot was used to assess the reactivity between anti Fc mAbs and denatured Ig fusion protein .

人血清白蛋白和人干扰素α2b的融合蛋白在毕赤酵母中的表达

Expression in Pichia pastoris and Properties of Human Serum Albumin-Interferon α 2b Chimera

人源抗HBsAg单链抗体与α干扰素融合蛋白酵母表达载体的构建

Construction of a Pastoris expression vector for a fusion protein consisting of anti-HBsAg scfv and interferon - α

电镜和TUNEL分析表明，颗粒酶B融合蛋白基因的诱导表达使部分细胞呈现凋亡的典型特征。

Electronic microscopy and TUNEL analysis showed that the inducible expression of granzyme B fusion protein gene caused typical features of apoptosis .

这说明，本研究用纯化的MCP融合蛋白制备兔抗OGNNVMCP抗体是成功的，并证实了OGNNV主衣壳蛋白的免疫原性。

The antibodies was successfully prepared with purified recombinant MCP .

SDS-PAGE和westernblot检测到带有Histag的约40kD融合蛋白中表达。

A 40 kD fusion protein was detected by SDS-PAGE and its His-tag was recognized by its MAb from mouse in Western-blot .

间接ELISA分析ScFv/tP融合蛋白的亲和活性，凝胶迁移阻滞实验检测ScFv/tP融合蛋白与DNA结合的情况。

The antigen-binding activity of the ScFv / tP fusion protein was confirmed by indirect ELISA , and the DNA binding ability was confirmed by EMSA .

HEVORF2重组菌菌种的稳定性及融合蛋白的应用

Stability of Recombinant Bacterium HEV Partial ORF_2 and Application of Recombinant Protein