传代细胞

乙型肝炎病毒重组DNA转化Vero绿猴肾传代细胞分泌乙型肝炎病毒表面抗原

Excretion of HBsAg by Vero cell line transformed by HBV recombinant DNA

人宫颈癌传代细胞Ig样蛋白的纯化分析

Purification and Western-blot analysis of Ig like protein from cervical cancer cell line

用MTT法检测硒化麒麟菜多糖对正常和肿瘤传代细胞的抑制作用。

The inhibitory action to normal and tumor cells of Seleno-polysaccharide of eucheuma by MTT method . 3 .

猫肾F（81）传代细胞中犬细小病毒原位PCR检测方法

Study of in situ Polymerase Chain Reaction Assay for Detection of Canine Parvovirus ( CPV ) in Feline Kidney F_ ( 81 ) Cells

通过试验，筛选出对水貂肠道冠状病毒敏感的貂肺传代细胞系（ML），经胰酶处理后，从6份病貂粪样中分离获得2株冠状病毒。

Two strains of coronavirus were isolated from 6 fecal samples of infected minks with mink lung cell line ( ML ) cultures .

免疫细胞化学染色示原代和传代细胞均为Nestin阳性。

Immunocytochemical method showed the primary and passage cells were nestin positive .

肾综合征出血热Vero传代细胞疫苗的研究

Studies on Vero Cell-derived Hemorrhagic Fever with Renal Syndrome Vaccine

结果：在原代及传代细胞中有Nestin阳性细胞即神经干细胞。

Results : Nestin positive cells were found in the culture and daughter clones .

结论HBV可以在体外感染滋养层细胞，且能在传代细胞中有持续的表达。

Conclusion The HBV can contaminate the JEG3 at vitro , and it was able to had the expression in spread layer cells with generations .

紫外线照射的HSV-2在人胚肺传代细胞上的持续感染

Human embryonic lung cells persistently infected by ultraviolet light - irradiated HSV-2

HFRS病毒Z（37）株在绿猴肾传代细胞的病变作用和传代适应

Cytopathic Effect ( CPE ) and Adaption of HFRS Virus Vaccine Strain E_ ( 37 ) in Green Monkey kidney Cell

结论：所建立的RTPCR方法可自病毒感染的小鼠脑组织和传代细胞中检测WEE病毒的基因组RNA。

Conclusions : A specific RT_PCR assay was established for detecting the genomic RNA of WEE virus from infected suckling mice and culture cells .

结果体外共育48~72h，LE抑制人肝癌细胞系的增殖；这种作用具有浓度依赖特点，而且相同浓度下不影响正常传代细胞的生长。

Results After co-culture for 48 ~ 72 h , LE inhibited the proliferation of human hepatoma cell characterized as concentration dependency .

目的：研究小剂量顺铂化疗联合放疗对实验性人子宫颈癌传代细胞（HeLa细胞）的增效作用。

Objective : To study low dose cisplatin with radiotherapy enhancing the experimental human cervical carcinoma passage cells ( HeLa cells ) radiosensivity .

分离培养的骨髓MSCs生长稳定，增殖力强，可选用前5代传代细胞作为软骨组织工程的种子细胞；

MSCs cultured in vitro show stable growth , rapid proliferation and 1 to 5 passage can be used as seed cells in cartilage tissue engineering .

本文通过免疫荧光（IF）和电镜（EM）技术对新疆出血热（XHF）及流行性出血热（EHF）病毒的生物学特性在veroE6及LLC-MK2两株传代细胞上进行了比较。

Some biological characteristics of the XHF virus and EHF virus cultured in Vero E_6 and LLC-MK_2 cells were studied by IF and EM.

从形态学变化、试验结果的重现性、稳定性和敏感性考虑，KB细胞可用作研究该毒素的传代细胞模型。

However , considering morphological changes , reproducibility and consistency , KB cells seemed to be a better potential model for evaluating cytotoxicity of microcystin-LR .

方法：采用狗肾传代细胞（MDCK）和鸡胚培养分离流感病毒，经血清学试验鉴定分型。

Methods : Viruses were isolated with MDCK cells and Chicken embryo and identified with serological test .

方法将BMSC传代细胞分别与PLA进行复合培养，观察细胞生长及其与生物材料附着情况，检测BMSC的增殖能力、蛋白含量及碱性磷酸酶（ALP）含量。

Method The BMSC attachment , extension and phenotype on PLA were observed and cell proliferation , alkaline phosphatase ( ALP ) activity was tested .

采用原代乳兔肾细胞、Vero及BHK（21）传代细胞，培养病毒均未成功。

The cell cultures of the virus did not meet with success ( milk rabbit kidney cell , Vero and BHK21 ) .

用流式细胞仪检测传代细胞的干细胞特异性标志CD57和P75的变化。

Each passengers were analyzed the specific marker CD 57 and LNGFR P 75 by FCM .

单抗标记FITC后用于人用鼠源性单抗制品及各种传代细胞和原代细胞中MAd检测，获得良好的实验结果。

Satisfactory results were obtained by using McAb-FITC for the detection of MAd virus potentially contaminated in primary and cultured cells and McAb of murine origin for human use .

采用Vero细胞（非洲绿猴肾细胞）分离病毒，传代细胞出现了明显的细胞变圆、脱落及拉网现象；

The Africa monkey kidney cell line ( Vero ) was used to isolate virus . The isolate caused cytopathic erfect ( CPE ) in Vero cells .

结论HFRSVero传代细胞疫苗的生产，疫苗株在Vero细胞中的连续传代适应起关键性作用。

Conclusion In the study on the HFRS purified vaccine in Vero cells , the most important thing is the passage adaptability of vaccinal strain in Vero cells .

以PCV-2阳性病料接种PK-15猪肾传代细胞，经分离和鉴定，获得PCV-2成都株。

PCV-2 Chengdu strain was obtained from PK-15 porcine kidney passage cells , which vaccinated by the positive samples .

结果经连续1年检测，HCV在传代细胞中持续存在，培养细胞中的HCVRNA负链和培养上清中的HCV呈间断阳性。

Results HCV positive strand RNA was detected in the cultured cells for 1 year . Interestingly , the HCV positive strand RNA was identified in supernatants and the negative strand RNA was also observed in the cultured cells intermittently .

方法收集非典型肺炎患者的各种临床标本，用狗肾传代细胞（MDCK）进行病原体分离，并运用血清学和分子生物学方法进行鉴定。

Methods Pathogens were isolated from variety of samples collected from atypical pneumonia patient by using MDCK cells , and identified with serological and molecular methods .

将股骨骨髓基质细胞进行原代和传代细胞培养，细胞计数法和噻唑蓝法绘制生长曲线，进行碱性磷酸酶（ALP）活性及体外矿化小结形成量的检测。

Bone marrow stromal cells from femur of rats were obtained for primary and secondary cultures , so as to investigate cell growth curve by cell counting and MTT assay , ALP activity and calcification nodule formation .

目的：观察国产冻干非洲绿猴肾传代细胞（Vero细胞）为基质制备的狂犬病纯化疫苗临床应用的免疫效果和安全性。

AIM : To observe the protective effect and the safety of freeze-dried rabies purified vaccine for human use ( Vero ) prepared from continuous cell line of African green monkey kidney ( Vero cell ) .

匐行恶丝虫（Dirofilariarepens）在我国犬体内首次发现犬传染性肝炎病毒在犬肾传代细胞中增殖过程的观察研究

FIRST REPORT OF DIROFILARIA REPENS FROM DOG IN CHINA Study on Reproduced Cycle of Infectious Canine Hepatitis Virus in a Dog Kidney cell Line