上清液

结果随CPP-ACP浓度的升高，培养基上清液的pH值升高，ΔpH降低，即变形链球菌的产酸量降低（P0.01）。

Results With the concentration of CPP-ACP increasing , the final pH value of supernate rose and the △ pH dropped , the acid production of Streptococcus mutans decreased ( P 0.01 ) .

结果表明2种融合蛋白Trx-Drs和Trx-Drs-lC大部分以可溶形式表达，可溶性表达的Trx-Drs在上清液中约占菌体总蛋白的22%。

The results showed that the fusion proteins , Trx-Drs and Trx-Drs-lC , were expressed as soluble proteins . The soluble expression level of Trx-Drs in the supernate amounted to 22 % of the total proteins of bacteria .

用生物化学方法测定上清液中MDA含量和抗氧化酶的活性。

Enzyme activities and malondialdehyde ( MDA ) were determined by biochemical assays .

ELISA法检测上清液中IL-2的水平；

The level of IL-2 in the supernatants was identified by ELISA .

成功地构建了人源化Fab的可溶性表达质粒，并用westernblot检测到其在上清液中有表达。

It was confirmed that Fab fragments expressed in supernatant by Western blot .

研究了不同上清液量对ZnO胶体球尺寸的影响。

The volume of adding supernatant has an impact on ZnO colloidal spheres size .

应用ELISA检测间质细胞培养基上清液中VEGF浓度？

The concentration of VEGF in the supernatant of the media was assayed by ELISA .

方法使用不同的酶标记物，观察ELISA法检测培养上清液中单链抗体的相对灵敏度。

Methods Relative sensitivity of various conjugates in ELISA for the detection of ScFv was assessed .

在发酵上清液中未检测到PVA氧化酶，但是检测到了PVA降解酶的总酶活和PVA脱氢酶。

PVA oxidase was not detected in the PVA-degrading supernatant .

实验结束后分别收集细胞上清液测定MDA，SOD和GSH-Px活性。

The supernatant was collected for detecting MDA , SOD , GSH-Px activity .

上清液中EPS及污泥中EPS均以多糖为主。

Both EPS in sludge and supernatant were mainly composed of polysaccharides .

ELISA法测定培养细胞上清液中FN的含量；

The level of fibronectin in the cultured supernatant was measured by ELISA .

用牛津杯实验筛选出抑菌能力不同的菌株，分别测定在不同培养时间，培养上清液的pH变化；

Selecting the strains with vary bacteriostatic ability by Oxford cup test , detecting pH of these strains cultured after different time .

鱼组织经匀浆、加热、离心、将上清液以包被液稀释后，与一抗、二抗反应，求得MT含量。

Fish tissues were homogenized , heated and centrifuged .

收集各组细胞培养上清液，采用ELISA方法检测各组细胞三种促血管形成因子分泌水平。

Collected supernatant of different cell lines , secretion levels of three angio-stimulating factors were detected by ELISA .

MSCs在成纤维细胞培养上清液联合EGF的体外培养条件下有向表皮细胞诱导分化的趋向。

MSCs have the trend to differentiate into epidermal cells in vitro .

用ELISA法检测上清液PGE2的含量。

The content of PGE2 in the supernatant was examined by ELISA .

采用双抗夹心ELISA法对上清液中IL-6进行定性和定量测定。

IL 6 in HECS was measured using sandwich enzyme linked immunosorbent assay ( ELISA ) method .

G值过大会导致絮凝体破碎，造成上清液中小体积絮凝体增多。

Oversized G value will lead to floc fragmentation , resulting in an increase in the supernatant of small and medium size floc .

方法对处于增殖期的C（57）BL/6小鼠单个核细胞（MNC）作氚标记亮氨酸（~3H-Leu）掺入处理，制备~3H-Leu标记的抗原上清液。

Methods Mononuclear cells from C57BL / 6 mice was labelled with ~ 3H-Leu to make Ag supernatant .

上清液的SEC分析结果也验证了上清液中含有溶菌酶分子三聚体。

The SEC result of supernatant also confirmed that the supernatant contained tri-molecular aggregate .

人红白血病细胞株K562上清液对人脐静脉内皮细胞的促增殖作用

Enhancement Effect of K562 Cells Supernatant on Proliferation of Human Umbilical Vein Endothelial Cells

ELISA方法检测细胞培养上清液中可溶性IL-2R（sIL-2R）水平。

The levels of sIL-2R in the supernatants were determined with ELISA .

在不同时期培养的上清液中均可较长时期检测到乙型肝炎病毒表面抗原（HBsAg）、乙型肝炎病毒e抗原（HBeAg）和乙型肝炎病毒DNA（HBV－DNA）。

In a longer period HBsAg , HBeAg and HBV-DNA can be detected in cultured supernatant fluid at different stages .

MBR上清液和污泥中的EPS以多糖为主。

The main component of EPS both in the supernatant and sludge were polysaccharide .

妊娠高血压综合征患者PBMC培养上清液中IL-4、IL-10的检测

Detection of IL-4 and IL-10 in cell culture supernatants of PBMC in pregnancy-induced-hypertension

目的探讨视网膜神经细胞培养的上清液对胚胎干细胞（embryonicstemcells，ES）体外分化的诱导作用。

Objective To investigate the possibility of commitment differentiation of embryonic stem cells induced by the medium of cultured retinal neurons of SD rats .

以ELISA法检测DCs培养上清液中IL-12的水平；

The levels of IL-12 in the culture supernatants were measured by enzyme-linked immunosorbent assay ( ELISA ) .

在体内CAM模型中，PSC上清液能使血管数目增加，管径增粗。

PSC media also increased the amount and the caliber of blood vessel in CAM model in vivo .

其发酵产物经离心分离后，上清液用无水乙醇分步沉淀抽提得到葡聚糖样品，然后用气相色谱（GC）、红外光谱（IR）、纸层析和化学方法等对葡聚糖样品进行鉴定。

The dextran sample which was extracted by ethanol precipitation and purified was assayed by gas chromatography ( GC ), infrared atlas , etc.