银染法

方法以PCR技术，聚丙烯酰胺凝胶垂直电泳和银染法对小卫星区域D17S30位点的扩增片段长度多态性进行了研究。

Methods Using PCR , polyacryl gel vertical electrophoresis and silver staining .

PCR结合银染法检测载脂蛋白E等位基因

PCR in combination with silver staining as a method for the determination of apolipoprotein E alleles

微卫星PCR聚丙烯酰胺凝胶银染法影响因素的分析研究

Analysis and Study of Influence Factors about Microsatellite PCR-polyacrylamide Gel-silver Staining

PCR产物8%聚丙烯酰胺凝胶电泳，凝胶采用银染法显色。

The products of PCR electrophoresed in8 % polyacrylamide gels .

用PCRTRAP法、聚丙烯酰胺凝胶电泳及银染法分析PCR产物，检测颌下腺标本中的端粒酶活性。

PCR TRAP was used to detect telomerase activity .

方法：用银染法和放射自显影法分别检测相同样本DNA的AR基因微卫星多态性，比较二者结果。

Methods : The microsatellite polymorphism of AR gene was detected by autoradiogram and silver stain .

方法DNA样本来自广东汉族男性无关个体，PCR后用聚丙烯酰胺凝胶电泳和银染法进行DNA分型。

Methods The DNA samples were amplified by PCR and analyzed by polyacrylamide gel electrophoresis followed by silver staining .

对固相校读PCR以及纳米金-银染法检测信号进行了初步摸索；

Solid-phase proofreading PCR and silver staining of colloidal gold were applied to detect the point mutation .

HPylori检测采用尿素酶试验法和Warthin&Starry银染法。

The status of H pylori was detected by warthin-starry silver staining and Urase test methods .

材料与方法：用TRAPELISA和TRAPPAGE银染法测定A375细胞端粒酶活性。

MATERIAL AND METHODS : TRAP_ELISA and TRAP_PAGE_silver staining .

体外转录生成核酶和靶RNA分子，进行切割反应后经变性聚丙烯酰胺凝胶电泳，银染法显色判定结果。

After in vitro cleaving reactions polyacrylamide gel electrophoresis was carried out followed by the silver nitrate staining .

结果：改良的银染法具有操作简便、对显影温度不苛求，耗费少等优点，所显示的DNA带型与传统的方法比较，同样清晰可读。

Results : The improved staining method was simple , economic and less dependent on temperature with the same sensitivity compared with the recommended ones .

这种复合银染法的灵敏度比单独使用银染法提高约1000倍，比单独使用考马斯亮兰R－250染色法提高约106倍。

The sensitivity of this complex silver stain was 1 000 times higher than silver stain and 106 times higher than coomassie blue stain .

应用PCR-SSCP银染法检测肺癌p53基因的突变

Study on Mutations of p53 Gene in Lung Cancer by PCR SSCP Silver Staining

PAGE银染法显示变形链球菌AP-PCR指纹图的可行性研究

PAGE-AgNO_3 staining displays AP-PCR fingerprint of mutans streptococci

银染法可以定性观察氨溴索对白假丝酵母菌成熟BF的影响。

Effects of ambroxol on mature BF of C. albicans were qualitatively observed under microscope by silver staining .

水稻AFLP指纹银染法显带研究

Improvement of AFLP Protocol for Silver-Staining in Rice

PCR-序列分析胶银染法快速、直接、简便、实用，适合脆性X综合征的大规模群体筛查。

PCR-Sequence gel silver staining is rapid , direct , simple , economic and suits to the colony screening fragile X syndrome on a large scale .

PCR－SSCP银染法检测胃和结、直肠癌APC基因突变

Detection of mutations of the adenomatous polyposis coli gene in gastric and colorectal carcinomas by PCR-SSCP analysis

豚鼠小肠EC细胞的免疫细胞化学和银染法的比较研究

A comparative study between immunocytochemical and silver staining methods on EC cells of the small intestine of the guinea pig

PCR-SSCP银染法在检测错配修复基因突变中的应用

The application of detecting mismatching repair gene mutation by the method of PCR-SSCP

方法：采用PCR－SSCP银染法对22例肺癌患者p53基因的第6、第7外显子进行分析。

Methods : The mutations of p53 gene exons 6-7 of 22 human lung cancer samples were assayed by PCR SSCP silver staining .

方法用PCR变性聚丙烯酰胺凝胶电泳银染法，检测50例肺癌组织Bax和TGFβRⅡ基因移码突变及微卫星改变。

Methods Frameshift mutations and microsatellite alterations were detected in 50 primary lung cancer tissues by PCR , 8 % denature polyacrylamide gel electrophoresis and silver staining .

用ELISA和RNAPAGE银染法检测了上海市第一人民医院新生儿病房及隔壁婴儿病房8份急性腹泻粪便标本；

Eight stool samples of acute diarrhea obtained from the new-born baby wards , the Shanghai First people 's Hospital were investigated by ELISA and RNA PAGE silver-staining method .

本实验以牛血清白蛋白为样品进行聚丙烯酰胺凝胶电泳，然后用考马斯亮兰R－250和银染法分步染色。

Bovine serum albumin was used as sample to carry out polyacrylamide gel electrophoresis and then was stained by using a complex silver stain that included coomassie blue stain and silver stain .

用PCRSSLP银染法分别分析了3个细胞系基因组中10个微卫星位点状况。

10 mononucleotide and dinucleotide microsatellite loci were analyzed by the PCR SSLP silver staining method .

银染法用于EMSA分析检测三链核酸形成

The Detection of Triplex DNA in EMSA Analysis by Silver Staining

方法：应用端粒重复扩增实验（telomeraserepeatamplificationprotocol，TRAP法）结合非变性聚丙烯酰胺凝胶电泳银染法测定35例大肠癌标本及正常黏膜端粒酶活性。

Methods : Combining telomerase repeat amplification protocol ( TRAP ) with PAGE silver staining , we detected the telomerase activity in cancer tissues and normal mucosa of 35 patients .

方法体外平板法制备Pa生物被膜模型，用银染法及扫描电镜鉴定。

Methods To establish the Pa biofilm model in vitro , and proved by a rapid silver nitrate staining procedure and scanning electronic microscope ( SEM ) .

方法：采用TRAP银染法，对32例原发头颈部鳞癌及15例癌旁组织进行端粒酶活性检测。

Methods : Thirty two patient samples of primary head and neck squamous cell carcinoma and 15 adjacent tissues were assayed using TRAP Silver staining for detection of telomerase activity .