酶促反应

基于有机溶剂中的酶促反应测定血清中维生素C含量

Determination of vitamin C in serum by peroxidase-based spectrophotometric method in isopropanol-water cosolvent medium

酶促反应中最适的pH及温度分别为7.5和30℃。

The optimal pH and temperature was 7.5 and 30 ℃ respectively .

PAP-H2O2-HRP酶促反应产物光谱及电化学研究

Spectroscopic and Electrochemical Studies on the Enzyme-catalyzed Reaction of PAP-H_2O_2-HRP

在序列间歇式活性污泥法（简称SBR）污水处理工艺中，水温会影响活性污泥微生物的酶促反应。

Water temperature affects micro-organism 's enzyme reaction in the SBR process .

方法：采用HPLC方法测定酶促反应的动力学参数及蒜酶的最适pH和最适温度。

Method : Analysis of the kinetic parameter of alliinase and the optimum pH and temperature by high performance liquid chromalography ( HPLC ) .

（木奈）果肉褐变是多酚类物质、多酚氧化酶（Polyphenoloxidase，PPO）与氧结合起酶促反应的结果。

Browning of pulps results from enzymatic reaction by integration of polyphenolics , polyphenol oxidase ( PPO ) and oxygen .

方法应用酶促反应及分光光度法测定60例脑膜瘤组织中碱性磷酸酶的含量，并利用手术前计算机断层扫描（CT）结果，比较60例脑膜瘤患者颅骨增生的发生情况。

Methods By enzymatic reaction and spectrophotometry , the contents of ALP in meningioma tissues were measured . The degree of the cranial hyperostosis was determined by the computer tomograph ( CT ) .

方法根据酶促反应速率原理，结合分光光度法，对CK和CK-MB的活性进行人工检测。

Methods The CK activity and CK-MB activity were measured using spectrophotometer based on the principle of enzymatic reaction rate .

酶促反应结果说明，当受体3′末端为核糖核苷时大大促进RNA连接酶的作用。

The results show that the single ribonucleoside substitution at the 3 ' - terminus of the acceptor greatly facilitates the joining of the oligodeoxynucleotide fragments by RNA ligase .

方法将（±）-美西律与大鼠肝微粒体孵育后，采用柱前衍生化毛细管气相色谱-氢火焰离子化检测法测定了酶促反应的时间曲线及动力学参数Vm和Km值。

METHODS The time course and the parameters of enzymatic kinetics of mexiletine enantiomers , V_m and K_m , were determined by a capillary gas chromatographic system with flame ionization detection .

有证据表明，植物中有几条潜在的产生NO的途径，包括一氧化氮合成酶（NOS）、硝酸还原酶（NR）、黄嘌呤氧化还原酶和非酶促反应途径。

There is evidence for several potential sources of NO in plants , including nitric oxide synthase ( NOS ), nitrate reductase ( NR ), xanthine oxidoreductase or nonenzymatic sources .

通过对不同底物对内胞霉PPO酶促反应速度的影响研究证实橡单宁的降解不仅与单宁酶有关还与多酚氧化酶有关。

By using model compound , catechol and pyrogallol , it was verified that at least both tannase and PPO were involved during degradation of tannins by the strain .

为了帮助克服这些困难，计算机科学进了生物界，并且发挥着越来越大的作用，如遗传信息的自动处理、DNA计算机的实现、生物酶促反应的模拟等。

In order to overcome these problems , computer science came into the field of biology , and become more and more important , such as the automatic process of genetic information , the implementation of DNA Computer and the simulation of enzyme induction reaction .

基于豆科植物的研究表明：豆腥味源于其中的多不饱和脂肪酸的酶促反应产物，其中起关键作用的酶是脂肪氧合酶（lipoxygenase，LOX）。

Studies on Leguminosae indicates that the bean flavor was caused by the products of enzymatic reactions using polyunsaturated fatty acids ( PUFAs ) as substrate .

pH-stat法在测定酶促反应米氏常数中的应用

Application of pH-stat Method on Determination of K_m of Enzymatic Hydrolysis Reaction

香菇甲醛是自身酶促反应的代谢产物，GGT和C-Slyase是其中两个关键酶。

Formaldehyde is a metabolite in Lentinus edodes ; GGT and C-S lyase were the two key metabolic enzymes in the formation of formaldehyde .

25℃以雄酮为底物，以NAD+和thioNAD+为辅因子时，融合蛋白参与酶促反应的最适pH分别为105和94。

With androsterone as the substrate and NAD + and thio NAD + as the cofactor , the optimum pH for the enzymatic reaction at 25 ℃ was 10 5 and 9 4 respectively .

以胸主动脉组织为对象，用酶促反应法测定CSE活性；

Using the thoracic aorta as study object , the enzymatic activity of cystathionine γ - lyase ( CSE ) was determined by enzyme facilitating reaction ;

本论文以猪胰岛素和苏氨酸叔丁酯为原料，通过酶促反应使B30丙氨酸换为苏氨酸叔丁酯，合成人胰岛素酯。

Porcine insulin was converted into human insulin ester with threonine-ester through transpeptidation catalyzed by enzyme .

目的：研究α-淀粉酶及其底物2-氯-4-硝基苯-麦芽三糖苷（CNP-G3）酶促反应过程中的激动剂、抑制剂、最适pH、米氏常数等。

Objective : To investigate the effects of agonist , inhibitor , optimum pH and km value in the enzyme-promoting course of reaction of amylase using 2-chloro-4-nitrophenyl-alpha-D-maltotrioside ( CNP-G3 ) as substrate .

酶促反应动力学曲线的分析结果，前20min酶的反应初速度呈线性关系；

The initial velocity of enzymatic reaction of HMG-CoA reductase was linear on the kinetic curve of enzymic reaction in the first 20 min.

提高纸浆湿强度的最佳酶促反应条件为：酶用量16U（以每克绝干浆计），温度45℃，pH值4.5，处理时间2h。

The inactivated laccase treatment resulted no change of tensile strength . The optimal enzymatic treatment conditions of improving the wet strength were : laccase dosage 16 U for 1 g pulp , temperature 45 ℃, pH value 4.5 and reaction time 2 h.

酶促反应过程中Brigg-Haldane稳态假说的适用性

The Brigg-Haldane Steady State Hypothesis Applied in the Process of Enzyme Catalytic Reaction

这种方法与通常SPR传感器检测技术不同，它消除通常当成检测目标的表面吸附信号，而检测通常认为是噪声的本体折射率变化信号，以此实时检测酶促反应的动态过程。

In contrast to traditional SPR detection and analysis method , this method monitoring an enzyme catalytic reaction by removing surface adsorption signals and detecting the SPR signals caused by the bulk reflective index changes in the enzyme-substrate solution .

本文阐述了乳链菌肽（Nisin）的特性及在鲜肉中的作用活性与肉中的谷胱甘肽的关系，推测Nisin很可能在原料肉中因与谷胱甘肽发生酶促反应而使其处于非活性状态。

The characteristic of Nisin and the relations between its activity and glutathion content of the fresh meat were introduced . Conclusion was deduced : probably because of the enzymatic reactions between Nisin and glutathion made it at non-active state .

通过加入金属离子络合剂乙二胺四乙酸（EDTA）进行了干扰消除实验，结果表明：可有效消除一定浓度的Zn2+对LDH酶促反应测定底物的干扰。

The experiment of the elimination of interference was affected by adding the ligand of metal ions-ethylene diamine tetraacetic acid ( EDTA ) . The result indicates that interference of certain concentration 's Zn2 + for determining substrates by the catalytic reaction of LDH can be eliminated effectively .

在非水相酶促反应中，离子液体的极性作用不遵从通常用来判别大多数有机物溶剂行为的规则，比如lgP规则。

Ionic liquids do not fit into most of the standard heuristics that chemists traditionally use to assess and predict solvent behavior , for example , the role of solvent lgP in non-aqueous enzymatic reactions .

利用离子液体作为粘合剂制备碳纳米管电极（CNTs-ILE），采用安培法对酶促反应进程进行持续动态的检测。

Using a new composite electrode fabricated with carbon nanotubes and ionic liquids ( CNTs-ILE ), continuous dynamic amperometric detection of the enzymatic reaction was achieved .

该菌无细胞提取液能进行降解MC-RR和MC-LR的酶促反应，并有最终产物的积累。

It was demonstrated that the enzymes in cell-free extract of this bacterium were responsible for the degradation of MC-RR and MC-LR , and the dead-end products of MC-RR and MC-LR catalyzed by these enzymes were observed on HPLC chromatograms .

以酶促反应膜分离耦合工艺制备的胶原蛋白多肽分子量均一，大小约为60kDa，应用于免疫试剂盒作为封闭剂比进口酪蛋白具有更好的封闭效果。

The molecular weight of collagen polypeptide by enzymatic-membrane separation coupling technology was homogeneous ( 60kDa ), which used as the blocking agent in immune diagnostic kits with better effect than casein .