无菌操作

无菌操作分离死亡2h内的胎儿下肢股骨、胫骨，贴壁筛选法分离培养胎儿骨髓间充质干细胞。

Lower limb femur and tibia were isolated from fetus corpse which died less than 2 hours by sterile operation and the primary human fetal mesenchymal stem cells ( hMSCs ) were acquired through adherent screening method .

加强无菌操作，减少补救时间，降低心内感染机会；

Sterile operation is necessary and it can reduce heart infection .

无菌操作培养人外周血淋巴细胞24h后，各组分别加入各浓度As2O3溶液及（或）姜黄素溶液。

The human peripheral blood lymphocyte be cultivated by aseptic technique approach for 24 hours . All groups be given different density As_2O_3 solution or curcumine solution .

结论TNA使用是安全的、可靠的。其配制须遵守无菌操作技术，配方科学准确。

Conclusion TNA is safe and reliable as long as it is prepared aseptically , scientifically without contamination .

结论神经外科机械通气患者VAP发生率高，医务人员应强化消毒隔离、无菌操作技术，合理使用抗生素。

Conclusion High VAP incidence happened in neurosurgery patients using mechanical ventilation . It is important to strengthen aseptic techique and select reasonable antibiotics .

结论做好外周中心静脉血管的选择，掌握封管压力，严格进行无菌操作，做好管道的护理是提高PICC成功的关键。

Conclusion The current site of puncture , proper blockading pressure , strictly aseptic manipulation and good nursing are the key points to increase the success .

提示临床工作中需合理使用抗生素、加强病房管理，严格执行无菌操作及洗手制度，预防医院感染发生，降低ICU患者医院感染发生率。

Therefore , medical staffs must use antibiotics rationally , strengthening the ward management , strictly implement aseptic technique and hand-washing system , preventing hospital infection , reducing the incidence of infection in ICU patients .

柱高5cm的DEAE纤维素柱用pH8.0的甘氨酸缓冲液作洗脱液，提纯子孢子回收率为63.2%，但严格无菌操作困难；

Column of DEAE cellulose with pH 8.0 buffered glycine solution as an eluent also enable to purify E. tenella sporozoites , except for the difficulties in sterilization as compared with the former procedure .

方法：常规消毒及无菌操作下，对1994～1998年产房的空气、用品及医护人员手取样细菌培养，并统计5a间新生儿及产妇感染率。

Methods : The sample of the air , articles in the delivery room and the hands of doctors and nurses there is detected by bacterial culture from 1994 to 1998 . The infective rate of the neonate and the parturient is statistic .

无菌操作麻醉下截除双侧桡骨中段1cm，缝合伤口，3个月后摄X线片证实骨不连模型形成。

The middle piece of bilateral radius ( 1 cm ) was extracted under anesthesia and aseptic technique , the wound was sutured , and the formation of nonunion model was proved by posterior X-rays film .

方法：对22例难治性VKC患者局部应用0.02mg/mlMMC滴眼，4次/d，配合其他眼部药物治疗，严格无菌操作，观察用药反应。

Methods : Local application of 0.02mg/ml MMC such as eye drip ( 4 times per day ) was used with aseptic processing to treat 22 cases of difficult VKC , combining with other medication .

方法经鼻内窥镜无菌操作下，于中鼻道采取分泌物标本，直接接种于血液培养基上，VITEK全自动微生物分析仪进行鉴定。

Methods Through nasal endoscope asepsis operation , Nasal secretion smear of middle meatus were inoculated on blood culture medium , we used VITEK automatic microbe analysis instrument to judge results .

新的袋子是细胞培养的无菌操作变的容易。

The new bags facilitate the sterile handling of cell cultures .

做无菌操作前，你应作什么准备？

What preparations should you make before conducting aseptic technique ?

术后注重营养支持，引流管的局部护理应严格无菌操作；

Nutritional support and aseptic technic in local care should be emphasized .

92.78%的护士能够认真执行无菌操作原则。

92.78 % nurses can abide by the rules of aseptic technique .

亚铵制浆黑液非无菌操作连续发酵生产酵母

Yeast production from spent ammonium sulfite liquor by continuous fermentation under non-aseptic conditions

请在使用该分离液时进行无菌操作。

Use aseptic techniques when handling this medium .

严格执行无菌操作可避免细菌性静脉炎的发生。

Strict sterile manipulation can avoid bacterial phlebitis .

86.60%的护士在进行无菌操作前、后能够洗手或使用消毒液；

86.60 % of them wash their hands or use disinfectant before and after aseptic manipulation .

应当按无菌操作或特殊要求取样。

The sampling shall be done in line with the aseptic technique or the special requirements .

隔离、加强无菌操作是防止感染扩散的有效措施；

Isolation of the patients , and sterilized operation are most important to prevent infection spreading .

结论严格无菌操作，规范监测管理是降低及控制医院感染的重要措施。

CONCLUSIONS Strict sterilizing procedures and standardized monitoring are the important measures to reduce the nosocomial infection .

所有瘤内注射均严格遵守无菌操作，采取多点注射。

All intratumoral injection of sterile operation are strictly adhered to , to multi-point injection . 4 .

这主要是通过灭菌工艺或者无菌操作程序的验证来完成。

This is accomplished primarily by validation of the sterilization process or of the aseptic processing procedures .

有数个研究对已更衣的无菌操作人员的污染强度进行了评估。

The contamination source strength of the gowned aseptic processing operator has been estimated in several research studies .

结论：认为术前精心细致的准备，术中密切有序的配合，严格无菌操作是手术成功的关键。

Conclusion : Sufficient preparation of apparatus , close operation cooperation and strict aseptic technique can improve cooperation quality .

注重无菌操作的训练，提高操作水平是降低污染率的重要手段。

Stressing the training of the germfree operation and raising the operation skill are important to lower the pollution rate .

严格无菌操作，严密止血，正确使用抗生素，防治切口感染或裂开。

Prevent and treat infection or rupture of incision by strict aseptic manipulation , hemostasis and right use of antibiotics .

采集的茶树的根、茎和叶经表面消毒后，在无菌操作条件下分离内生真菌，并进行纯化。

The roots , stems and leaves of tea plant are surface disinfected , we isolated endophytic fungi by sterile working .