逆转录病毒感染

人类急性髓系白血病逆转录病毒感染的研究

Investigation of retroviral infection in human acute myeloid leukemia

方法采用绿色荧光蛋白（greenfluorescentprotein，GFP）逆转录病毒感染人RPE细胞。

Methods The immortalized human RPE cells were genetically labeled by retrovirus vector carrying a green fluorescent protein ( GFP ) .

将重组假逆转录病毒感染2215细胞，ELISA及斑点杂交法分别检测被感染细胞培养上清中HBsAg、HBeAg及HBVdna。

In infected 2 2 15 cells , the levels of HBsAg , HBeAg and HBV DNA were determined by ELISA and dot hybridization , respectively .

重组逆转录病毒感染人Hela细胞48h时，MTT实验测得细胞死亡率为53.2%。

MTT assay demonstrated a cell death rate of 53.2 % 72 h after transfection of Hela cells with the plasmid .

STF-PERT对及时发现逆转录病毒感染，提高临床输血安全性有着十分重要的意义。

STF-PERT will be useful in retrovirus screen timely and be great significance for transfusion security .

目的通过人工改变多聚腺苷酸化信号的重组逆转录病毒感染人胃上皮细胞GES-1，检测病毒转录能否产生病毒-宿主融合转录产物及其是否具有转化胃上皮细胞GES-1的能力。

Objective To verify if mutated polyadenylation signal retroviruses can produce viral-host readthrough transcripts ( Rth ) and have the ability to transform human gastric epithelial GES-1 cells , and to discuss the new functions of retroviruses in gastric cancer related gene research .

双嗜性逆转录病毒感染日本血吸虫细胞的生物学理论与可行性探讨

Biological theory and experimental foundation for transduction of cultured Schistosoma japonicum cells with amphotropic retrovirus

传统的制作转基因动物方法有显微注射法、逆转录病毒感染法和胚胎干细胞法等，但每种方法都有其缺陷，限制了其在今后转基因动物研究中的广泛应用。

Microinjection , retrovirus mediated method and embryonic stem cells method were all the traditional methods of producing transgenic animals . But these methods had defects and limited application in the research of transgenic animals in the future .

由双嗜性包装细胞产生的GFP逆转录病毒能够感染原代与建株的人RPE细胞，GFP基因可持续在RPE细胞内表达。

The GFP retrovirus was able to infect primary cultured human RPE cells and immortalized RPE cell line .

但是TRIM5a不能防止像HIV-1这样的逆转录酶病毒感染，而当前艾滋病病毒正在人群中蔓延。

But TRIM5a could not stop infection by retroviruses that currently infect humans , such as HIV-1 .

方法用携带单纯疱疹病毒胸苷激酶（HSV－tk）基因的重组逆转录病毒，感染大鼠神经胶质瘤细胞C6，筛选稳定表达tk的克隆细胞C6／tk；

Methods C6 rat glioma cells were infected with recombinant retrovirus containing HSV tk gene . Following selection the C6 / tk cell line which stably expressed tk was cloned .

猪内源性逆转录病毒体外感染人胚肾细胞系HEK-293的初步研究

Preliminary study on human embryonic kidney cell line HEK-293 after porcine endogenous retrovirus infection

LTR碎片最初通过逆转录病毒的感染形成，这种病毒类型可将本身的遗传物质与宿主的基因结合为一体。

LTR fragments were originally formed by infection with retroviruses , a type of virus that can integrate their own genetic material into a host gene .

方法：用逆转录病毒载体感染鼠源性膀胱癌细胞株T739，体外观察其对GCV的敏感性。

Method : HSV-TK gene was transferred into murine bladder cancer cell line T739 by retrovirus in vitro , its sensitivity to GCV was observed .

包装成逆转录病毒，感染小鼠肝癌细胞MM45T。

The retrovirus carrying IL-18 gene was used to infect murine liver cancer cell MM45T .

方法：用RV-LNSX，RV-LMP1和RV-LMP1TRADD逆转录病毒分别感染CNE2细胞，G418筛选后，观察和检测转基因细胞的形态学特点，在基质中的运动迁移能力以及E-钙黏蛋白（E-Cadherin）表达的变化；

After selection with G418 , the morphology , the ability of motion and migration in extracellular matrix , expression of LMP1 and E-Cadherin in transgenic cells were observed or detected .

蜘蛛需要通过携带遗传信息的逆转录酶病毒来感染受试者，接着通过遗传信息来改变细胞中的DNA，而且几乎人体内的每个细胞都要被感染。

The spider would need to infect the subject with a retrovirus , which carries genetic information . The genetic information would then have to alter the DNA in cells . Almost every cell in the body would have to be infected .

结论成功构建了PIG-A基因逆转录病毒载体，感染突变型K562细胞后可以提高其CD59的表达。

Conclusion The retrovirus vector containing the PIG-A gene was successfully con - structed and the expression of CD59 on the mutant K 562 cells could be increased .

结论以逆转录病毒为载体感染的稳定表达rhIL-17F的293T转基因细胞株的首次获得，为进一步研究hIL-17F的生物学功能及其机制奠定了良好的基础。

Conclusion This is the first time for us to obtain the transgenic 293T cells expressing stably rhIL-17F protein in China , which paves the way for future study on biological functions and mechanism of hIL-17F .

高效抗逆转录病毒治疗艾滋病毒感染者和艾滋病患者的6年临床疗效及安全性观察

Therapeutic effect and safety evaluation on 6-year highly active antiretroviral therapy for Chinese HIV-1 infected patients

这个研究小组于2月8日在美国丹佛举行的2006年逆转录病毒和机会感染会议上公布了他们的研究成果。

The team presented its findings yesterday ( 8 February ) at the2006 Conference on Retroviruses and Opportunistic Infections in Denver , United States .

南非的一位结核病专家最近号召让所有得不到抗逆转录病毒药物的感染艾滋病病毒的儿童服用廉价且容易获得的药物预防结核病。

A South African tuberculosis expert has called for a cheap and readily available drug for tuberculosis prevention to be given to all HIV-positive children not receiving antiretroviral treatment .

在那一天，（美国）约翰霍普金斯大学的黛博拉·佩尔绍德在佐治亚州的亚特兰大召开的逆转录病毒和机会性感染会议上表示，她已治愈一位患有艾滋病毒的病人。

On that day Deborah Persaud of Johns Hopkins University announced to the Conference on Retroviruses and Opportunistic Infections in Atlanta , Georgia , that a patient under her care had been cured of HIV infection .

其中的一个亚类是组织样记忆B细胞，这类B细胞在没有接受抗逆转录病毒疗法的HIV感染者体内含量特别丰富。

One of the sub-category is organizations like memory B cells , B cells in the absence of such anti-retroviral therapy of HIV infection especially rich in content .

中国猕猴D型逆转录病毒（SRV）感染猴体的病理学改变

Pathological variation of Chinese rhesus monkeys infected by simian retrovirus D

猴D型逆转录病毒（SRV）感染恒河猴诱导脑细胞凋亡

Apoptosis of cell in brain of rhesus monkeys infected by simian retrovirus D

来自两个国家的早期数据显示，如果接受抗逆转录病毒治疗，合并感染H1N1和艾滋病毒的患者没有严重或致命疾病的更高风险。

Early data from two countries suggest that people co-infected with H1N1 and HIV are not at increased risk of severe or fatal illness , provided these patients are receiving antiretroviral therapy .

用高效抗逆转录病毒治疗HIV/HCV重叠感染33例

Highly active anti-retroviral Therapy on 33 cases of HIV / HCV superinfection

通过抗逆转录病毒治疗，艾滋病毒感染者可以过上健康和丰富的生活。

With ART , HIV-infected individuals can live healthy and productive lives .

此外，本论文亦为研究逆转录病毒跨种间感染和宿主的抗病毒固有免疫提供了重要的实验论据。

Furthermore , the results of this study present useful data on the cross-species transmission of retroviruses and the antiretroviral innate immunity of hosts .