内源基因

花椰菜ACC氧化酶基因的克隆及其RNAi对内源基因表达的抑制作用

Cloning of ACC Oxidase Gene and Inhibition of Endogenous Gene Expression with RNAi in Cauliflower

结果表明，建立的多重PCR方法能够准确的同时检测出豆粕中的内源基因和转基因成分。

The results showed that the high efficiency PCR system can differentially , conveniently , effectively and coinstantaneously examine the endogenous gene Lectin and the transgenic components .

利用改良的CTAB法，对多种大豆（GlycineMax）加工产品DNA进行分离纯化，并以大豆特异性内源基因大豆凝集素基因为参照，对用该方法获得的DNA的可扩增性加以验证。

A modified CTAB method for DNA purification from processed genetically modified soybean ( glycine max ) products ( GMO ) was adopted .

这些iPS细胞中，内源基因和端粒酶也激活不足。

These iPS cells also show insufficient activation of endogenous pluripotent genes and telomerase genes .

孟山都一种应用RNA干涉技术来调控内源基因表达的转基因黄豆，已经在等待美国农业的审批。

Monsanto GM soya beans , which use RNA interference to modulate the expression of endogenous genes , are already awaiting a decision from the USDA .

RNA干扰（RNAi）是指生物体内由小干扰RNA（siRNAs）介导的具有高度特异性，只降解与之序列相应的单个内源基因mRNA的，在进化上高度保守的转录后基因沉默现象。

RNA interference ( RNAi ), an evolutionary highly conserved process of posttranscriptional gene silencing , can be triggered by small interfering RNAs ( siRNAs ) that mediate sequence-specific mRNA degradation .

针对大豆色拉油中DNA较难提取的问题提出了充分乳化、延长醇沉淀时间和反复富集小片段DNA等措施，有效地从大豆色拉油中提取到DNA，并通过大豆特异内源基因扩增得以证明。

DNA was efficiently isolated from soybean oil through several measures of efficient emulsification , elongating the time of ethanol precipitation and repeat concentration of small DNA fragments . It was proved that DNA was isolated successfully by doing PCR of soybean native gene lectin ;

分子诊断是以DNA、RNA或蛋白质分子为诊断材料，通过检查人体内源基因或外源（病原体）基因的存在、缺陷或表达异常，对人体状态或疾病作出特异性诊断的方法和过程。

Molecular diagnosis is the method and process of which specific diagnosis of the body state and disease is made by examining the existence , defects or abnormal expression of both the internal genes and external genes ( pathogens ), with DNA , RNA and protein molecule as diagnostic material .

最后以环介导等温扩增法比照QPCR的标准方法，进行特异性和灵敏度实验，筛选得到了对2个内源基因，4个外源基因检测的环介导等温扩增法引物。

Finally , loop-mediated isothermal amplification method cf QPCR standard methods , the specificity and sensitivity experiments , was screened for two endogenous genes , 4 ring detection of exogenous gene mediated isothermal amplification primers .

它通过诱变使特定内源基因发生过量表达，而产生显性功能获得型突变，从而对基因进行鉴定和分析。

It can generate dominant gain-of-function mutants by overexpression of a particular endogenous gene .

另外，内源基因的高效表达可能会影响外源蛋白的产量。

In addition , the efficiently expressing of endogenous gene may affect the production of heterologous protein .

依赖于同源识别的基因沉默，是指向生物体内导入外源核酸时引起相应序列的内源基因的表达被特异性抑制的一种基因调控现象。

It is sequence specific inhibition of corresponding endogenous gene expression induced by foreign nucleic acid molecules .

对小片段定量分析结果表明，不同条件干热处理后转基因棉粕外源基因与内源基因的拷贝数比没有明显的变化规律。

The quantitative analysis of small fragments showed that the copies ratio in different conditions of dry treatment heat had no significant tendency .

该内源基因表达调控单元用于表达所选的特定异源编码基因以及特别用于表达异源抗原。

The endogenous gene expression regulatory units are used to express particular chosen heterologous coding sequences and in particular to express heterologous antigens .

植物的开花除了受诸多内源基因的调控外，还受外界环境因子（如光照和温度）的影响。

Flowering of plants is not only regulated by mutiple endogenous genetic factors , but also controlled by complex environmental factors , such as light and temperature .

此外，本研究所分析的所有转基因后代中，内源基因转录本和目标性状均有相当程度的残留，而且表现出了株间、籽粒间和环境间的变异。

Furthermore , in this study , all transgenic progenies showed considerable remaining of target gene expression and target traits , and variations among plants , seeds and environments were also considerable .

本研究以家蚕为研究对象，对目前家蚕实时定量PCR研究中常用的内源参照基因进行分析，与双跟踪标定定量PCR方法进行比较。

This research take the Bombyx mori as the object of study , through the current real-time quantitative PCR study of silkworm commonly used endogenous reference gene analysis , and compare with the dual-spike-in qPCR method .

病毒诱导基因沉默是利用RNA介导病毒防卫机制的一项技术，构建含有目的基因片段的人工改造病毒载体，通过农杆菌侵染导致植物内源目的基因沉默。

Virus-introduced gene silencing is a technique that exploits the RNA-mediated antiviral mechanism . The modified virus vector carrying the fragment of a target gene were constructed and then inserted to silence the target gene within host plants by Agrobacterium-meditated transformation .

从印度豆粕、中国豆粕中仅检出内源参照基因Lectin，确定了PCR技术检测抗草甘膦转基因豆粕的灵敏度为0·5%，稳定性良好。

However , no other gene but the endogenesis gene Lectin is found in Indian and Chinese Soybean Meal . The sensitivity of the detection of glyphosate-resistant Genetically Modified Soybean Meal by PCR-based method is 0.5 % and the stability is fairly good .

利用建立的双引物PCR技术同时检测内源和外源基因，检出来自美国、阿根廷的大豆及饲料为转基因阳性产品，取得良好效果。

Based on the detection with double-primer PCR , the soybeans and feedstuffs from America and Argentina were GM positive .

除探索转化利用内源光敏色素基因，提高玉米耐阴性的可能性外，也有助于研究光敏色素A在光信号转导系统的有关机理及作用。

In addition to exploration of transferring endogene and improvement of shade tolerance ability , it also help to study the mechanism and function of phytochrome A of the maize .

由于这些抗菌蛋白都源于植物内源表达的基因，所以从转基因植物安全性的角度上考虑更易于被群众接受、消除他们对转基因食物的疑虑心理。

It is also possible that such genes are safer than induced genes when used in transgenic plants and are thus potentially more acceptable given the current public opposition to genetic modified food .

它能特异地拮抗内源或外源基因的表达，这为研究某特定基因功能提供了更为有效的方法，为培育抗病植株、调控次生代谢和农艺性状改良提供了可能途径。

It can inhibit endogenote and exogenote expression particularly which provides a effective method for studying the faction of a certain specific gene and provides a possible pathway to breeding disease-resistence plants , modulating and controlling secondary metabolism , modifying agricultural character .

然而，至今仍不知道内源表达Harpin编码基因是否也能增强植物耐旱性。

So far it is still unknown whether genetic transformation of Harpin-encoding genes improves plant tolerance to drought .

植物内源激素与不同基因型小麦抗寒性关系的研究

Study on Relations Between Plant Endogenous Hormones and Cold Resistance in Wheat

在内源几丁质酶活力相对较低的情况下，转导外源基因可掩盖或抑制内源基因的表达；

When the chitinase activity in the wild type was low , exogenous gene can cover up or inhibit endogenous gene expression .