连续切片

方法：采用连续切片及图像分析的方法观察、测量了18例SD大鼠房室来的内部纤维构筑。

Method : The internal architecture of His bundle were observed and measured by serial section and image analysis in 23 rats .

所有标本均常规连续切片，厚度为sop。

All the specimens were routine serial section , whose thickness was 5 $ m.

胃腺癌（gastricadenocarcinoma，GC）25例。所有标本均经10%中性甲醛固定，常规脱水浸蜡，石蜡包埋，连续切片。

All specimens were fixed in10 % paraformaldehyde , dehydrated , waxed , embedded in paraffin , sliced inseries .

对人喉标本连续切片和CT图像进行综合处理，输入计算机中进行三维重建。

By using serial section of human larynxes and CT images , a three-dimensional reconstruction of larynx is made with the computer system-SUN 4 / 75 work station .

连续切片HE染色的对照观察显示妊娠黄体中大、小黄体细胞均可出现OT免疫阳性反应。

OT immunoreactivity in CL tissue during pregnancy could be observed both in large luteal cells and in small luteal cells .

方法：分别用ABPAS，AB（pH2.5），HID法对25例病理诊断为肠化的贲门组织的5μm连续切片3张进行染色。

Methods : 25 cases of IM was detected by AB PAS , AB ( pH 2 5 ), HID , respectively .

在6例OA滑膜标本连续切片中，滑膜的衬里层细胞、炎症浸润细胞以及血管内皮细胞中均强烈表达iNOS蛋白。

In synovial consecutive slice samples of 6 cases from OA , iNOS was predominantly expressed in synovial lining cells , inflammatory infiltrating cells , endothelial cells .

通过椎体成形手术前后CT扫描，分别得到了各90张连续切片，层厚1mm，每个像素的尺寸是0.33mm。

Based on CT scanning before and after PVP , 90 serial sections with the thickness of 1 mm were obtained and the size of each pixel was 0.33 mm .

在胰腺癌标本中用TdT酶标记的DNA3/-OH末端分析连续切片表明，大多数癌细胞未发生细胞凋亡，在40例胰腺癌中仅有3例发现约10%的癌细胞凋亡。

Apoptotic signal was present in approximately 10 % of the tumor cells in only 3 of 40 cancer samples .

结果：大鼠全程CNS连续切片各断面上神经元呈深棕黄色，核膜及核仁呈黑色；

RESULTS : Neurons on each section of the whole CNS serial sectioning in rat were in dark nankeen , which the nuclear membrane and nucleolus were in black ;

常规酒精脱水，二甲苯透明，浸蜡包埋，5μm连续切片，编号备用，载玻片涂APES胶，以防脱片。

Conventional alcohol dehydration , xylene transparent , the Baptist embedded in wax , 5 μ m consecutive section , part numbers and standby .

做矢状面5μm连续切片，每个标本切3张，分别用于AQP1免疫组化染色、阴性对照和HE染色，用已知有AQP1表达的大鼠肾脏肾小管切片为阳性对照。

Then , 5 μ m serial section was made on the sagittal plane .

经连续切片以不同第一抗体孵育结果得出：NPY与亮啡肽在肾上腺髓质中的分布特点不一致，部分NPY阳性细胞与亮啡肽有细胞内共存现象。

The results of incubation with different primary antibodies NPY , DBH , Leu-Enk on serial sections showed that NPY-like immunoreactivity co-exists with Leu-Enk in part of chromaffin cells .

采用连续切片法进行鸡胚胎显微观察，研究鸡原始生殖细胞（PGCs）迁移特性及其与性腺发生、发育的关系。

The relationship between PGCs migration and gonad development of chick embryo which was incubated for 3 hours ~ 7 days were studied .

方法：利用Golgi染色、超薄连续切片及NIH图像分析系统测量海马CA1区锥体细胞树突棘头及突触后致密体的大小。

Methods : Golgi impregnation , consecutive ultrathin sections and NIH imaging were used to measure the size of the spine head and its postsynaptic density .

对临床已确诊的2例皮内痣进行连续切片，痣细胞和基底膜的表达分别采用S－100多克隆抗体和IV型胶原单克隆抗体LAB－SA免疫组化染色法。

Taken from 2 paraffin embedded tissues , pigmented nevi were cut into serial histologic sections . The expression of nevus cell and basement membrane were respectively applied by S-100 and IV collagen antibody with LAB-SA immunohistochemical technique .

病理检测中连续切片（SS）+免疫组化（IHC）同苏木精（HE）染色的假阴性率分别为7%、23%。

Pathological examinations false negative rates in serial section ( SS ) + immunohistochemistry ( IHC ) and hematoxylin and eosin ( HE ) staining were 7 % and 23 % respectively .

将雄性胎鼠以4卜m厚作冠状位连续切片，HE染色，光镜观察睾丸引带组织学变化：免疫组织化学方法检测睾丸引带中＊＊＊mI＊＊A的表达。

The specimens of male offspring were sliced serially in coronal plane , expression of ACTIN and PCNA in gubernaculum testis was detected by immunohistochemical methods , histological changes were observed with light microscope ( LM ) .

扫描后处死裸鼠，取肿瘤标本，漂洗、固定、石蜡连续切片，应用免疫组织化学方法从蛋白质水平观察膜相关抗原HAb18G在该模型上的表达。

After all imaging studies , the tumor specimens were taken out for immunohistochemical staining to observe the expression of HAb18G at protein level .

方法用还原型尼可酰胺腺嘌呤二核苷酸磷酸硫辛酰胺脱氢酶（NADPH-d）酶组织化学技术，对钉螺软体的整体连续切片作系统观察。

Methods By way of nicotinamide adenosine dinucleotide phosphate diaphorase ( NADPH d ) histochemical technique the continued sections of Oncomelania hupensis snails were investigated systematically .

结果：（1）连续切片的抗酸染色和PGL抗原检测可提高对早期皮损内抗酸菌（AFB）及PGL抗原的发现率。

Results : ( 1 ) Senstivity of AFB and PGL antigen detection can be significantly improved by examination of 20 or more serial sections .

利用改进的GMA半薄连续切片技术对水稻品种IR36胚囊形成和发育进行了研究。

The process of embryo sac formation and development in rice ( cultivar IR36 ) was studied by the modified GMA semi-thin serial sectioning technique .

方法取5～12天的鸡胚端脑固定后，采用石蜡包埋，制成5μm厚连续切片，进行硫瑾染色和TUNEL染色光镜观察。

Methods Chick telencephalons from day 5 to day 12 of embryonic development were fixed and paraffin embedded , serially sectioned at 5 μ m , stained with Thionin and TUNEL respectively and observed in the light microscope .

方法采用大鼠大脑中动脉（MCA）阻断模型阻断血流2小时后再灌注，分别在不同再灌注时间将大鼠断头取脑，连续切片分别作HE、TUNEL染色及bcl-2蛋白免疫组化染色。

Method The middle cerebral artery ( MCA ) of rats was occluded for 2 h with MCAO model , and reperfusion . At each time rat brains were continuously cut to three sections for HE 、 TUNEL staining and bcl 2 protein immunohistochemical staining .

方法采用10、13、16d龄雄性大鼠各10只，制作火棉胶连续切片，应用计算机分析系统对下丘脑核团进行观察结果。

Methods 10,13,16 days of age in wister male rat , each 10.By means of celloidin embedding in coronal plane . Hypothalamic nucleus were measured on computer assisted morphometric techniques .

方法：从尸体（10具）上取三叉神经根标本，对三叉神经感觉根（TNSR）进行石蜡包埋及连续切片，并系统观察；

Methods : Two trigeminal nerve root and the trigeminal nerve sensory root ( TNSR ) were obtained from 10 anatomic cadav ers and were paraffin embedded .

方法：取30、60和120周apoE（鄄/鄄）小鼠心脏做连续切片，分别用HE染色、免疫组化、透射电镜鉴定冠状动脉外膜炎性细胞类型。

Methods : Each heart of 30 - , 60 - and 120-week-old apoE ( - / - ) mice was cut consecutively and stained by HE staining , immunohistochemistry staining and electron transmission microscope to identify the types of the imflammatory cells infiltrated at adventitia of coronary artery .

采用连续切片技术，研究了15～45期（孵化50h～19d）鸡早期胚胎发育过程中精原细胞与睾丸发生发育的关系。

Chicken embryos from stage 15 to stage 45 were studied by means of serial section and light microscopy in order to learn the relationship between the spermatogonium and the testicular development in the early chicken embryo .

实验用成年大白鼠，切除其双侧顶叶正中部大脑皮质，术后立即腹腔注射~3H-胸腺嘧啶核苷，第4天取大脑组织，Carnoy液固定，连续切片，焦油紫染色。

Intraperitoneal injection of H ~ 3-thymidine was given to the rats immediately after corticectomy of the parietal lobes . On the 4th day after operation , the cerebral tissue was removed , fixed in Carnoy , serial sectioned , and stained with cresyl violet .

切除实验狗全喉，连续切片观察切除范围和手术后创面修复情况。

Dimension of excision and wound surface recovery status were observed .