载体蛋白
- carrier protein
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酰基载体蛋白(acylcarrierprotein,ACP)是一类具有保守的丝氨酸残基的小分子量的酸性蛋白质。
Acyl carrier protein ( ACP ) is a small acidic protein with a conservative serine residue .
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然后通过碳二亚胺法将半抗原与载体蛋白偶联制备人工抗原,采用紫外扫描及SDS-PAGE鉴定。
Then , antigen was prepared by conjugation of hapten with carrier protein by carbodiimide ( EDPC ) method and was identified by UV spectroscopy and SDS-PAGE .
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C57BL/6小鼠胆结石形成中固醇载体蛋白2的mRNA水平和胆固醇饱和指数增高
MRNA of SCP 2 and cholesterol saturation index are increased during gallstone formation in C57BL / 6 mice
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植物ZIP基因家族铁载体蛋白基因研究进展
Advance of ZIP gene family related to iron transporter in plant
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白血病细胞经典的耐药机制涉及到ABC(ATPBindingCassette,ATP结合盒)型膜载体蛋白家族。
ABC ( ATP Binding Cassette ) membrance bearer protein is involved in MDR .
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牛心肌线粒体ADP/ATP载体蛋白的分离及应用
Isolation of the ADP / ATP carrier from beef heart mitochondria and its application
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ABC类载体蛋白(ATPBindingCassette)家族中的药物转运蛋白影响许多药物的药代动力学过程。
Active drug transporters of the ATP binding cassette ( ABC ) - containing family of proteins have a major impact on the pharmacokinetics of most drugs in use today .
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采用活性酯法将CL1分别与载体蛋白BSA、OVA共价偶联,制备了人工抗原Ⅰ(CL1BSA)和包被原Ⅰ(CL1OVA);
Was activated by N-Hydroxysuccinimide and conjugated with BSA and OVA to prepare artificial antigens I ( CL_1 - BSA ) and coating antigen I ( CL_1-OVA ) .
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脂多糖结合蛋白(LipopolysaccharideBindingProtein,LBP)是一种促进LPS与效应细胞受体相结合的关键载体蛋白,在炎症反应过程中具有重要的作用。
Lipopolysaccharide Binding Protein ( LBP ) is a kind of emergency reaction proteins that conduct the binding of lipopolysaccharide to its receptor on the target cells . Therefor LBP plays an important role in inflammatory reaction .
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方法设计一个具有双重抗凝功能的重组多肽分子结构,它由谷胱甘肽S转移酶(GST)作为载体蛋白与重组抗凝多肽融合而成。
Methods We designed a construct using glutathione S transferase ( GST ) as a protein vector , fused via a cleavable linker to an antithrombotic peptide of 31 amino acids .
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利用混合酸酐法将DES-HS与载体蛋白(BSA,OVA)进行偶联得到DES人工抗原。
The artificial antigen of DES was synthesized by mixed anhydride method using DES-HS and bovine serum albumin ( BSA ) and ovabumin ( OVA ) respectively .
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CL是一种小分子有机物,本身不具备免疫原性,不能直接用于免疫实验动物,必须将其与大分子载体蛋白偶联,才能刺激动物的免疫机制产生特异性抗体。
CL is a micromolecule organics without immunogenicity . So it is unable to be used of immunization of animal experiment directly . To stimulate zoic immunologic mechanism producing specific antibody , CL must be coupled with macromolecule carrier protein .
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从偶联位点、偶联剂、载体蛋白和偶联步骤等分析出发,设计了微囊藻毒素-LR(Microcystin-LR,MC-LR)完全抗原的制备方法。
Based on the analysis of coupling location , coupling regent , carrying protein and coupling process , completed antigen of Microcystin-LR ( MC-LR ) was synthesized .
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葡萄糖转运蛋白-1(GLUT-1)是哺乳动物细胞葡萄糖跨膜转运的主要载体蛋白,与代谢密切相关。
Glucose transporter-1 ( GLUT-1 ) is the main carrier protein of transporting the glucose in mammalian cell , which is closely correlated with metabolism .
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方法:本课题分三部分进行:1.抗PTX的抗血清的制备将PTX与载体蛋白KLH、BSA偶联成具有免疫功能的PTX-PDP-KLH免疫抗原和PTX-PDP-BSA检测抗原。
METHODS : This article is composed of three parts : 1 . Preparation of anti-PTX antiserum . PTX was coupled with the carrier protein KLH and BSA to provide PTX the immunogenicity .
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以己烯雌酚(DES)、琥珀酸酐和牛血清白蛋白(BSA)等为主要原料,采用混合酸酐反应(氯甲酸异丁酯法)将半抗原己烯雌酚与载体蛋白BSA联接制备成人工免疫原。
Using diethylstilbestrol ( DES ), succinic anhydride and bovine serum albumin ( BSA ) as main stuff , the artificial-immunogen has been obtained by the mixed acid anhydride reaction ( isobutyl chloroformate method ) to connect hapten DES with carrier-protein BSA .
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而且发现将HBD-2成熟肽与载体蛋白TRX直接融合也可以实现其在37℃的高可溶性表达。
It was also found that if mature HBD-2 was directly fused to the fusion partner TRX , fusion product could be expressed efficiently with high solubility even at 37C .
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以混合酸酐法将DES-MCPE与载体蛋白偶联形成完全抗原,并以紫外扫描分析了完全抗原的偶联比率。
The conjugates of DES-MCPE with proteins as complete antigens were prepared by the mixed anhydride method and the conjugation rate of them were analyzed by UV scanning .
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首先将FTB的C6位羟基与琥珀酸酐反应,制备出FTB琥珀酸单酯化合物(FTB-hemisuccinate,FTBS),经元素分析和光谱分析后,再用常规方法与3种载体蛋白脱水结合形成酰胺化合物。
First the hydroxyl group at C-6 position of FTB was reacted with hemisuccinate , forming the FTB-hemisuccinate ( FTBS ), then the compound was identified by ultimate and spectrometric analysis . The FTBS was combined with three carrier proteins respectively to form the amide compound .
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由重组E.colirPE553D所表达的基因缺失突变脱毒的重组铜绿假单胞菌外毒素A(rEPA),目前大量以载体蛋白用于多种细菌多糖蛋白结合疫苗研究。
The recombinant pseudomonas aeruginosa exotoxin A ( rEPA ), expressed in E. coli BL21 (λ DE3 ) with a plasmid bearing mutant EPA gene deleted the code for key amino acid 553D ( glutamine 553 site ), has been widely applied in polysaccharide-protein conjugate vaccine research .
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乙肝核心抗原作为免疫载体蛋白的研究进展
Advance on Hepatitis B Virus Core Antigen as a Vaccine Carrier Moiety
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畜禽血液甲状腺激素及其载体蛋白的研究进展
The Study on Serum Thyroid Hormone and Their Binding Proteins in Livestock
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草莓果实钾载体蛋白基因的克隆与序列分析
Molecular Cloning and Analysis of Potassium Transporter Gene in Strawberry
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吗啡&载体蛋白交联物的鉴定及抗吗啡单克隆抗体的药理学评价
Characterization of Morphine-carrier Protein Conjugate and Pharmacological Evaluation of Anti-morphine Monoclonal Antibody
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不同载体蛋白制备的A群脑膜炎球菌多糖结合物的免疫原性比较
Immunities of Meningococcal Serogroup A Polysaccharide-Protein Conjugate Vaccines Prepared with Different Carrier Proteins
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运动对葡萄糖转运载体蛋白4基因表达的调控
Regulation of glucose transporter 4 gene expression during exercise
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表皮生长因子介导的基因转移载体蛋白的制备
Expression and Purification of Gene Transfer Vehicle Mediated by Epidermal Growth Factor Receptor
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水溶性壳聚糖纳米载体蛋白药物的体外释放模型
Release Model of Water-soluble Chitosan Nanoparticles for Protein Delivery
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植物脂酰-酰基载体蛋白硫酯酶研究综述
Literature Review of Plant Fatty Acyl-Acyl Carrier Protein Thioesterase
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结论:醛化法能使毒素GTX2,3与载体蛋白偶联。
Conclusion : The method of aldehyde can conjugate GTX2,3 with BSA and KLH .